EC 1.14.14.3 Chemical Properties

storage temp. 

-20°C

form 

powder

Water Solubility 

Water: 50 mg/mL

EPA Substance Registry System

Luciferase (9014-00-0)

Safety Information

WGK Germany 

3

F 

3-10-21

MSDS

• Language:English Provider:SigmaAldrich

EC 1.14.14.3 Usage And Synthesis

Uses

Luciferase may be used:

• to treat mammary tumor cryosections for bioluminescence imaging studies
• as a component of luciferase reaction mixture
• in bioluminescence assay membrane preparations of Zymomonas mobilis

Uses

Luciferase has been used as a component in Mix B respiration medium for the determination of the ATP synthetic activity of the H⁺-ATP synthase. It has also been used in thermal aggregation to assay CnoX (YbbN) activity.

Uses

Luciferase from Vibrio fischeri has been used in a study to assess kinetics of light emission and oxygen consumption by bioluminescent bacteria. It has also been used in a study to investigate the sensitivity of dark mutants of various strains of luminescent bacteria to reactive oxygen species.

General Description

Alkanal reduced FMN-oxygen oxidoreductase (1-hydroxylating, luminescing)

Biochem/physiol Actions

Luciferase catalyzes the conversion of reduced flavin mononucleotide in the presence of oxygen and long-chain aldehyde to flavin mononucleotide, visible light and a fatty acid. It is a reporter enzyme for sensitive quantitation. The lucifearse fusion proteins have been useful in many protein based interaction studies especially in living cells.

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