EC 1.14.14.3
EC 1.14.14.3 Basic information
- Product Name:
- EC 1.14.14.3
- Synonyms:
-
- BACTERIAL LUCIFERASE
- EC 1.14.14.3
- LUCIFERASE, BACTERIAL
- LUCIFERASE
- LUCIFERASE FROM PHOTOBACT. FISCHERI, LYO PHIL., POWDER
- LUCIFERASE BACTERIAL FROM VIBRIO*FISCHER I
- LUCIFERASE FIREFLY RECOMBINANT 95+%
- LUCIFERASE FROM PH. FISCHERI PARTIALLY PURIFIED POWDER
- CAS:
- 9014-00-0
- MW:
- 0
- EINECS:
- 232-751-7
- Mol File:
- Mol File
EC 1.14.14.3 Chemical Properties
- storage temp.
- -20°C
- form
- powder
- color
- White to light yellow
- Water Solubility
- Water: 50 mg/mL
- EPA Substance Registry System
- Luciferase (9014-00-0)
Safety Information
- WGK Germany
- 3
- F
- 3-10-21
MSDS
- Language:English Provider:SigmaAldrich
EC 1.14.14.3 Usage And Synthesis
Uses
Luciferase may be used:
- to treat mammary tumor cryosections for bioluminescence imaging studies
- as a component of luciferase reaction mixture
- in bioluminescence assay membrane preparations of Zymomonas mobilis
Uses
Luciferase has been used as a component in Mix B respiration medium for the determination of the ATP synthetic activity of the H+-ATP synthase. It has also been used in thermal aggregation to assay CnoX (YbbN) activity.
Uses
Luciferase from Vibrio fischeri has been used in a study to assess kinetics of light emission and oxygen consumption by bioluminescent bacteria. It has also been used in a study to investigate the sensitivity of dark mutants of various strains of luminescent bacteria to reactive oxygen species.
General Description
Alkanal reduced FMN-oxygen oxidoreductase (1-hydroxylating, luminescing)
Biochem/physiol Actions
Luciferase catalyzes the conversion of reduced flavin mononucleotide in the presence of oxygen and long-chain aldehyde to flavin mononucleotide, visible light and a fatty acid. It is a reporter enzyme for sensitive quantitation. The lucifearse fusion proteins have been useful in many protein based interaction studies especially in living cells.
in vivo
Luciferase (labels HEK293 cells stably expressing pmeLUC；i.p. or intramass injection) can measure the ATP concentration in the tumor microenvironment in real-time in the mouse tumor model inoculated with HEK293 cells stably expressing pmeLUC[1].
Luciferase can clearly monitor the growth of cancer cells in a mouse model in which RENCALUC cells are inoculated subcutaneously[2].
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EC 1.14.14.3(9014-00-0)Related Product Information
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- FLUORESCEIN-5-THIOSEMICARBAZIDE
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- Tosylmethyl isocyanide
- METHYL ISOCYANOACETATE
- SALCOMINE
- 2,4-PENTANEDIONE, SILVER DERIVATIVE
- Ferric acetylacetonate
- COBALT(II) ACETYLACETONATE
- N-BUTYLISOCYANIDE
- TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)EUROPIUM(III)
- PHENYLSELENOL
- DICHLORO(ETHYLENEDIAMINE)PLATINUM(II)
- 1,1,3,3-TETRAMETHYLBUTYL ISOCYANIDE
- TERT-BUTYL ISOCYANIDE
- Aluminum acetylacetonate