7-AMINO-4-METHYL-3-COUMARINYLACETIC ACID Chemical Properties

Boiling point:

512.2±50.0 °C(Predicted)

Density 

1.391±0.06 g/cm3 (20 ºC 760 Torr)

storage temp. 

Keep in dark place,Sealed in dry,Room Temperature

solubility 

DMF: soluble

form 

powder to crystal

pka

3.80±0.10(Predicted)

color 

Light yellow to Brown

Appearance

Solid

λmax

344nm(Buffer)(lit.)

BRN 

7927205

InChI

InChI=1S/C12H11NO4/c1-6-8-3-2-7(13)4-10(8)17-12(16)9(6)5-11(14)15/h2-4H,5,13H2,1H3,(H,14,15)

InChIKey

QEQDLKUMPUDNPG-UHFFFAOYSA-N

SMILES

C1(=O)OC2=CC(N)=CC=C2C(C)=C1CC(O)=O

Safety Information

Hazard Codes 

Xi

Risk Statements 

36/37/38

Safety Statements 

26-36

WGK Germany 

3

HS Code 

29322090

MSDS

• Language:English Provider:SigmaAldrich

7-AMINO-4-METHYL-3-COUMARINYLACETIC ACID Usage And Synthesis

Description

AMCA is fluorescent protein labeling agent. It contains an N-hydroxysuccinimide ester that reacts with lysine residues to form photostable amide links. Upon activation with UV light, AMCA displays emission maxima of 400-460 nm. It has commonly been used in multiplex immunophenotyping applications.

Uses

7-AMINO-4-METHYL-3-COUMARINYLACETIC ACID is a blue fluorescent dye. Its desirable properties include a relatively large Stoke's shift and resistance to photobleaching. 7-AMINO-4-METHYL-3-COUMARINYLACETIC ACID is used as contrasting probes for double and triple labeling in im

Uses

Non-Activated, Amine-Reactive, fluorescent probe

Chemical Reactivity

Primary amines (needs activation)

References

[1] H. KHALFAN. Aminomethyl coumarin acetic acid: A new fluorescent labelling agent for proteins[J]. Journal of Molecular Histology, 1986, 18 9: 497-499. DOI: 10.1007/bf01675617
[2] G L FERRI. Quadruple immunofluorescence: a direct visualization method.[J]. Journal of Histochemistry & Cytochemistry, 1997, 45 2: 155-158. DOI: 10.1177/002215549704500201
[3] P. M. NEDERLOF. Three-color fluorescence in situ hybridization for the simultaneous detection of multiple nucleic acid sequences[J]. Cytometry Part B: Clinical Cytometry, 1989, 10 1: 20-27. DOI: 10.1002/cyto.990100105
[4] ANDRé GOTHOT  Jean M P  Jean Claude Grosdent. A strategy for multiple immunophenotyping by image cytometry: Model studies using latex microbeads labeled with seven streptavidin-bound fluorochromes[J]. Cytometry Part A, 1996, 24 3: 214-225. DOI: 10.1002/(sici)1097-0320(19960701)24:3<214::aid-cyto4>3.0.co;2-h

Abs/Em Maxima

345/450 nm

Extinction Coefficient

19,000 cm-1M-1

Spectrally Similar Dyes

Alexa Fluor® 350, AMCA, DyLight® 350

7-AMINO-4-METHYL-3-COUMARINYLACETIC ACID(106562-32-7)Related Product Information

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