AMCA-NHS Chemical Properties

Boiling point:

559.4±60.0 °C(Predicted)

Density 

1.50±0.1 g/cm3(Predicted)

storage temp. 

−20°C

solubility 

DMF: soluble

pka

1.69±0.20(Predicted)

form 

A solid

Appearance

Light yellow solid

ex/em

353/455 nm

ε(extinction coefficient)

19000 L⋅mol−1⋅cm−1

BRN 

9007679

InChI

InChI=1S/C16H14N2O6/c1-8-10-3-2-9(17)6-12(10)23-16(22)11(8)7-15(21)24-18-13(19)4-5-14(18)20/h2-3,6H,4-5,7,17H2,1H3

InChIKey

ILKQVTIMOGNSAS-UHFFFAOYSA-N

SMILES

C1(=O)OC2=CC(N)=CC=C2C(C)=C1CC(ON1C(=O)CCC1=O)=O

Safety Information

Hazard Codes 

Xi

Risk Statements 

36/37/38

Safety Statements 

26-36

WGK Germany 

3

F 

8-10-21

HS Code 

3212900000

MSDS

• Language:English Provider:SigmaAldrich

AMCA-NHS Usage And Synthesis

Description

Activated NHS-ester of AMCA (aminomethyl coumarin acetate) dye. This NHS ester is an amine-reactive dye for labeling of amine groups in proteins, peptides, amino-modified oligos, and other target molecules.

AMCA is one of the brightest blue fluorescent dyes. This fluorophore has a relatively large Stoke’s shift, high resistance to photobleaching, and pH-independent fluorescence from pH 4 to 10. AMCA is a widely used fluorophore for multiple-color labeling due to its minimal fluorescence overlap with green- and longer wavelength-emitting fluorescent dyes.

Chemical Properties

Appearance: Pale yellow solid
ex/em: 353/455 nm
Extinction coefficient (ε): 19000 L??mol??1??cm??1

Uses

AMCA-H NHS ester reacts with primary amines at pH 7.0-9.0. Suitable for labeling lysyl residues in proteins.

Abs/Em Maxima

348/435 nm

Fluoresene quantum yield

0.91

Extinction Coefficient

17400

CF260

0.16

CF280

0.13

AMCA-NHS(113721-87-2)Related Product Information

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• (E)-Ethylidenesuccinic acid
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• Coumarin 120
• CHEMBRDG-BB 4023581
• 7-AMINO-4-METHYL-3-COUMARINYLACETIC ACID
• AMCA-NHS