MUNG BEAN NUCLEASE Chemical Properties

storage temp. 

-20°C

form 

lyophilized powder

color 

Colorless to light yellow

PH

8.0 (25°C in water)

biological source

Serratia marcescens

Specific Activity

≥250units, pH 8.0, 37°C

Safety Information

Safety Statements 

23-24/25

WGK Germany 

3

F 

10-21

MSDS

• Language:English Provider:SigmaAldrich

MUNG BEAN NUCLEASE Usage And Synthesis

Uses

The enzyme has been used for an assay to detect the amount of Tf1 RNA that is protected from degradation by the nuclease, Benzonase. The Tf1 element of Schizosaccharomyces pombe is a long terminal repeat-containing retrotransposon that encodes functional protease, reverse transcriptase, and integrase proteins. It has also been used in the fractionation of nuclear pellet and nuclear extract obtained from cell lysates.

Uses

The enzyme from Sigma has been used to limit cell clumping during the preparation of chimeric cell mixtures. It has also been used for the preparation of nuclear extracts by digesting DNA and releasing nuclear proteins intimately associated with DNA.

Uses

Turbonuclease from Serratia marcescens has been used for cell lysis during proximity biotinylation assay (BioID) and affinity-purification. It has also been used as a component of lysis buffer for protein extraction from cell lines for affinity purification studies.

General Description

Endonuclease from Serratia marcescens is a dimer containing two identical monomeric units with distinct protein folds. The core contains a six-stranded antiparallel β-sheet flanked by α-helices on either side. Each monomer bears one active site. This enzyme is a magnesium-dependent nucleases.

Biochem/physiol Actions

Benzonase? is a genetically engineered endonuclease from Serratia marcescens. The protein is a dimer of 30 kDa subunits with two essential disulfide bonds. This endonuclease attacks and degrades all forms of DNA and RNA (single stranded, double stranded, linear and circular) and is effective over a wide range of operating conditions. It completely digests nucleic acids to 5′- monophosphate terminated oligonucleotides 3 to 5 bases in length. This is ideal for the removal of nucleic acids from recombinant proteins. It can also be used for applications where complete digestion of nucleic acids is desirable. It also reduces viscosity in protein extracts and prevents cell clumping. Pre-treatment of a protein sample with this enzyme improves its resolution on 2D gel electrophoresis by eliminating any bound nucleic acids. The optimum pH for enzyme activity is found to be 8.0-9.2.

Description

Benzonase Nuclease Serratia Marcescens Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 245 amino acids and having a molecular mass of 30kDa with 2 essential disulfide bonds.

Benzonase Nuclease is purified by proprietary chromatographic techniques.

Source

Escherichia Coli

Specificity

Unspecific (DNA, RNA) attacks all nucleic acids (single strand, double strand, circular, supercoiled) with no apparent sequence preference. Final reaction product:

5’-mono-phosphate terminated oligonucleotides (3-5 bases).

Protease Activity: Not det

Background

Serratia marcescens secretes an endonuclease that has exceptionally high specific activity to the medium that surrounds it. The Benzonase Nuclease is mainly used for elimination of nucleic acid contamination from purified proteins, downstream processing, reduction of viscosity etc. Nucleic acid contaminants are caused by nuclease released to the medium. The DNA is being destroyed by the release of the S. marcescens nuclease and it acts as the killer gene for the auto destruction of microorganisms.

MUNG BEAN NUCLEASE(9025-65-4)Related Product Information

• nuclease micrococcal, Staphylococcus aureus,MICROCOCCAL NUCLEASE,NUCLEASE
• BENZONASE(R) NUCLEASE,BENZON NUCLEASE,BENZONASE NUCLEASE,NUCLEASE,MUNG BEAN NUCLEASE,NUCLEASE S1 SOLUTION,MUNG BEAN NUCLEASE
• BENZONASE NUCLEASE,BENZONASE(R) NUCLEASE,BENZON NUCLEASE,MUNG BEAN NUCLEASE,NUCLEASE,NUCLEASE-GAMMA,MUNG BEAN NUCLEASE
• nuclease alpha
• single-strand-specific nuclease, endoplasmic reticulum
• UV incision nuclease
• staphylococcal nuclease R
• nuclease, shrimp
• Thermus filiformis nuclease
• ANTI-APEX NUCLEASE
• nuclease C1
• Aspergillus Nuclease S1
• nuclease gamma, Ustilago maydis
• sugar-nonspecific nuclease
• streptococcal nuclease
• Albumine bovine nuclease-free
• CEL I nuclease
• nuclease H