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MUNG BEAN NUCLEASE

Product Name
MUNG BEAN NUCLEASE
CAS No.
9025-65-4
Chemical Name
MUNG BEAN NUCLEASE
Synonyms
NUCLEASE;BENZONASE;NUCLEASE R;Binuclease;EC 3.1.30.1;EC 3.1.30.2;S1 NUCLEASE;NUCLEASE S1;NUCLEASE P1;ENDONUCLEASE
CBNumber
CB4664699
Formula Weight
0
MOL File
Mol file
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MUNG BEAN NUCLEASE Property

storage temp. 
-20°C
form 
lyophilized powder
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Safety

Safety Statements 
23-24/25
WGK Germany 
3
10-21
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Hazard and Precautionary Statements (GHS)

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
T4330
Product name
Turbonuclease from Serratia marcescens
Purity
recombinant, expressed in E. coli
Packaging
50000units
Price
$356
Updated
2024/03/01
Sigma-Aldrich
Product number
E8263
Product name
Benzonase® Nuclease, ultrapure
Purity
≥250?units/μL, ≥99% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution, ultrapure grade
Packaging
5000units
Price
$173
Updated
2023/06/20
Sigma-Aldrich
Product number
E8263
Product name
Benzonase® Nuclease, ultrapure
Purity
≥250?units/μL, ≥99% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution, ultrapure grade
Packaging
25000units
Price
$594
Updated
2023/06/20
Sigma-Aldrich
Product number
E1014
Product name
Benzonase® Nuclease
Purity
≥250?units/μL, ≥90% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution
Packaging
5000units
Price
$74.2
Updated
2022/05/15
Sigma-Aldrich
Product number
E1014
Product name
Benzonase® Nuclease
Purity
≥250?units/μL, ≥90% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution
Packaging
25000units
Price
$258
Updated
2022/05/15
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MUNG BEAN NUCLEASE Chemical Properties,Usage,Production

Uses

The enzyme has been used for an assay to detect the amount of Tf1 RNA that is protected from degradation by the nuclease, Benzonase. The Tf1 element of Schizosaccharomyces pombe is a long terminal repeat-containing retrotransposon that encodes functional protease, reverse transcriptase, and integrase proteins. It has also been used in the fractionation of nuclear pellet and nuclear extract obtained from cell lysates.

Uses

The enzyme from Sigma has been used to limit cell clumping during the preparation of chimeric cell mixtures. It has also been used for the preparation of nuclear extracts by digesting DNA and releasing nuclear proteins intimately associated with DNA.

Uses

Turbonuclease from Serratia marcescens has been used for cell lysis during proximity biotinylation assay (BioID) and affinity-purification. It has also been used as a component of lysis buffer for protein extraction from cell lines for affinity purification studies.

General Description

Endonuclease from Serratia marcescens is a dimer containing two identical monomeric units with distinct protein folds. The core contains a six-stranded antiparallel β-sheet flanked by α-helices on either side. Each monomer bears one active site. This enzyme is a magnesium-dependent nucleases.

Biochem/physiol Actions

Benzonase? is a genetically engineered endonuclease from Serratia marcescens. The protein is a dimer of 30 kDa subunits with two essential disulfide bonds. This endonuclease attacks and degrades all forms of DNA and RNA (single stranded, double stranded, linear and circular) and is effective over a wide range of operating conditions. It completely digests nucleic acids to 5′- monophosphate terminated oligonucleotides 3 to 5 bases in length. This is ideal for the removal of nucleic acids from recombinant proteins. It can also be used for applications where complete digestion of nucleic acids is desirable. It also reduces viscosity in protein extracts and prevents cell clumping. Pre-treatment of a protein sample with this enzyme improves its resolution on 2D gel electrophoresis by eliminating any bound nucleic acids. The optimum pH for enzyme activity is found to be 8.0-9.2.

MUNG BEAN NUCLEASE Preparation Products And Raw materials

Raw materials

Preparation Products

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MUNG BEAN NUCLEASE Suppliers

Jiangsu Jinpu Nuoan Biotechnology Co., Ltd
Tel
0512-82709698 15250590826
Email
marketing@gproan.com.cn
Country
China
ProdList
9
Advantage
58
Shanghai Macklin Biochemical Co.,Ltd.
Tel
15221275939 15221275939
Fax
021-50706099
Email
shenlinxing@macklin.cn
Country
China
ProdList
15885
Advantage
55
Sigma-Aldrich
Tel
021-61415566 800-8193336
Email
orderCN@merckgroup.com
Country
China
ProdList
51471
Advantage
80
Shanghai YuanYe Biotechnology Co., Ltd.
Tel
021-61312847; 18021002903
Fax
QQ:3008007432
Email
3008007409@qq.com
Country
China
ProdList
27322
Advantage
60
Chengdu Youngshe Chemical Co., Ltd.
Tel
+86-17380623303 +86-17380623303
Fax
028-62328193
Email
Caroline@youngshechem.com
Country
China
ProdList
2359
Advantage
58
Beijing OKA biological technology co., LTD
Tel
010-010-62971590 18548936886
Fax
010-62340519
Email
3462612863@qq.com
Country
China
ProdList
6912
Advantage
58
Guangzhou Tomums Life Science Co., Ltd.
Tel
020-31155029 18902330969
Fax
020-31155029
Email
sales@tomums.cn
Country
China
ProdList
4698
Advantage
58
Henan Alfa Chemical Co., Ltd
Tel
+8618339805032
Email
alfa4@alfachem.cn
Country
China
ProdList
13039
Advantage
58
Wuhan Rongcan Biotechnology Co., Ltd
Tel
18327024450
Fax
183-27024450
Email
2130026275@qq.com
Country
China
ProdList
4576
Advantage
58
career henan chemical co
Tel
+86-0371-86658258 15093356674;
Fax
0086-371-86658258
Email
factory@coreychem.com
Country
China
ProdList
29826
Advantage
58
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View Lastest Price from MUNG BEAN NUCLEASE manufacturers

Career Henan Chemical Co
Product
Nuclease, nucleateendo- 9025-65-4
Price
US $9.80/KG
Min. Order
1KG
Purity
≥98%
Supply Ability
20 tons
Release date
2020-01-09

9025-65-4, MUNG BEAN NUCLEASERelated Search:


  • endonuclease serratia marcescens,*recombinant 90+
  • BENZONASE
  • BENZONASE(R)
  • BENZONASE NUCLEASE
  • BENZON NUCLEASE
  • BENZONASE(R) NUCLEASE
  • EC 3.1.30.2
  • EC 3.1.30.1
  • ENDONUCLEASE
  • ENDONUCLEASE S1 (ASPERGILLUS ORYZAE)
  • IUB: 3.1.30.1
  • S1 NUCLEASE
  • NUCLEASE, MUNG BEAN
  • NUCLEASE-GAMMA
  • NUCLEASE
  • NUCLEASE S1 (ASPERGILLUS ORYZAE)
  • NUCLEASE S1
  • NUCLEASE R
  • NUCLEASE P1
  • MUNG BEAN NUCLEASE
  • BENZONASE (R) NUCLEASE, RECOMBINANT EXPRESSED IN ESCHERICHIA COLI
  • BENZONASE (R) NUCLEASE, ULTRAPURE GRADE, RECOMBINANT EXPRESSED IN ESCHERICHIA COLI
  • NUCLEASE FROM MUNG BEAN SPROUTS(PHASEOLUS AUREUS)
  • NUCLEASE FROM MUNG BEAN SPROUTS
  • benzonase, ultrapure
  • endonuclease from serratia marcescens
  • Benzonase(R) Nuclease, ultrapure
  • Turbonuclease
  • Benzonase? Nuclease, ultrapure
  • Turbonuclease from Serratia marcescens
  • Nuclease from Phaseolus aureus sprouts from mung bean
  • Benzonase<sup>(R)</sup> Nuclease
  • Nuclease(Endonuclease from Serratia marcescens)
  • Nuclease, nucleateendo-
  • Endonuclease Ⅰ
  • Ultra Nuclease( GMP class )
  • Benz-Neburase
  • Binuclease
  • Binuclease Liquid
  • Binuclease, Benzonase Nuclease
  • Broad spectrum Nuclease
  • UltraNuclease
  • 9025-65-4
  • BioChemical
  • Application Index
  • Biochemicals and Reagents
  • Cell Lysis and Protein Extraction Reagents
  • Metabolomics
  • Nucleotide Metabolism
  • Protein Sample Clarification
  • Protein &amp; Nucleic Acid Isolation
  • Proteomics
  • Proteomics and Protein Expression
  • Sample Preparation
  • General Metabolic Enzymes
  • 3.1.x.x Acting on esters
  • 3.x.x.x Hydrolases
  • Enzymes, Inhibitors, and Substrates