ChemicalBook > CAS DataBase List > MUNG BEAN NUCLEASE

MUNG BEAN NUCLEASE

Product Name
MUNG BEAN NUCLEASE
CAS No.
9025-65-4
Chemical Name
MUNG BEAN NUCLEASE
Synonyms
NUCLEASE;BENZONASE;NUCLEASE R;Binuclease;EC 3.1.30.1;EC 3.1.30.2;S1 NUCLEASE;NUCLEASE S1;NUCLEASE P1;ENDONUCLEASE
CBNumber
CB4664699
Formula Weight
0
MOL File
Mol file
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MUNG BEAN NUCLEASE Property

storage temp. 
-20°C
form 
lyophilized powder
color 
Colorless to light yellow
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Safety

Safety Statements 
23-24/25
WGK Germany 
3
F 
10-21
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Hazard and Precautionary Statements (GHS)

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
T4330
Product name
Turbonuclease from Serratia marcescens
Purity
recombinant, expressed in E. coli
Packaging
50000units
Price
$356
Updated
2024/03/01
Sigma-Aldrich
Product number
E8263
Product name
Benzonase® Nuclease, ultrapure
Purity
≥250?units/μL, ≥99% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution, ultrapure grade
Packaging
5000units
Price
$173
Updated
2023/06/20
Sigma-Aldrich
Product number
E8263
Product name
Benzonase® Nuclease, ultrapure
Purity
≥250?units/μL, ≥99% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution, ultrapure grade
Packaging
25000units
Price
$594
Updated
2023/06/20
Sigma-Aldrich
Product number
E1014
Product name
Benzonase® Nuclease
Purity
≥250?units/μL, ≥90% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution
Packaging
5000units
Price
$74.2
Updated
2022/05/15
Sigma-Aldrich
Product number
E1014
Product name
Benzonase® Nuclease
Purity
≥250?units/μL, ≥90% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution
Packaging
25000units
Price
$258
Updated
2022/05/15
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MUNG BEAN NUCLEASE Chemical Properties,Usage,Production

Uses

The enzyme has been used for an assay to detect the amount of Tf1 RNA that is protected from degradation by the nuclease, Benzonase. The Tf1 element of Schizosaccharomyces pombe is a long terminal repeat-containing retrotransposon that encodes functional protease, reverse transcriptase, and integrase proteins. It has also been used in the fractionation of nuclear pellet and nuclear extract obtained from cell lysates.

Uses

The enzyme from Sigma has been used to limit cell clumping during the preparation of chimeric cell mixtures. It has also been used for the preparation of nuclear extracts by digesting DNA and releasing nuclear proteins intimately associated with DNA.

Uses

Turbonuclease from Serratia marcescens has been used for cell lysis during proximity biotinylation assay (BioID) and affinity-purification. It has also been used as a component of lysis buffer for protein extraction from cell lines for affinity purification studies.

General Description

Endonuclease from Serratia marcescens is a dimer containing two identical monomeric units with distinct protein folds. The core contains a six-stranded antiparallel β-sheet flanked by α-helices on either side. Each monomer bears one active site. This enzyme is a magnesium-dependent nucleases.

Biochem/physiol Actions

Benzonase? is a genetically engineered endonuclease from Serratia marcescens. The protein is a dimer of 30 kDa subunits with two essential disulfide bonds. This endonuclease attacks and degrades all forms of DNA and RNA (single stranded, double stranded, linear and circular) and is effective over a wide range of operating conditions. It completely digests nucleic acids to 5′- monophosphate terminated oligonucleotides 3 to 5 bases in length. This is ideal for the removal of nucleic acids from recombinant proteins. It can also be used for applications where complete digestion of nucleic acids is desirable. It also reduces viscosity in protein extracts and prevents cell clumping. Pre-treatment of a protein sample with this enzyme improves its resolution on 2D gel electrophoresis by eliminating any bound nucleic acids. The optimum pH for enzyme activity is found to be 8.0-9.2.

MUNG BEAN NUCLEASE Preparation Products And Raw materials

Raw materials

Preparation Products

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MUNG BEAN NUCLEASE Suppliers

China 78 Germany 1 Japan 3 Switzerland 1 United Kingdom 1 United States 17 Global 101
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View Lastest Price from MUNG BEAN NUCLEASE manufacturers

Career Henan Chemical Co
Product
Nuclease, nucleateendo- 9025-65-4
Price
US $9.80/KG
Min. Order
1KG
Purity
≥98%
Supply Ability
20 tons
Release date
2020-01-09

9025-65-4, MUNG BEAN NUCLEASERelated Search:

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  • endonuclease serratia marcescens,*recombinant 90+
  • BENZONASE
  • BENZONASE(R)
  • BENZONASE NUCLEASE
  • BENZON NUCLEASE
  • BENZONASE(R) NUCLEASE
  • EC 3.1.30.2
  • EC 3.1.30.1
  • ENDONUCLEASE
  • ENDONUCLEASE S1 (ASPERGILLUS ORYZAE)
  • IUB: 3.1.30.1
  • S1 NUCLEASE
  • NUCLEASE, MUNG BEAN
  • NUCLEASE-GAMMA
  • NUCLEASE
  • NUCLEASE S1 (ASPERGILLUS ORYZAE)
  • NUCLEASE S1
  • NUCLEASE R
  • NUCLEASE P1
  • MUNG BEAN NUCLEASE
  • BENZONASE (R) NUCLEASE, RECOMBINANT EXPRESSED IN ESCHERICHIA COLI
  • BENZONASE (R) NUCLEASE, ULTRAPURE GRADE, RECOMBINANT EXPRESSED IN ESCHERICHIA COLI
  • NUCLEASE FROM MUNG BEAN SPROUTS(PHASEOLUS AUREUS)
  • NUCLEASE FROM MUNG BEAN SPROUTS
  • benzonase, ultrapure
  • endonuclease from serratia marcescens
  • Benzonase(R) Nuclease, ultrapure
  • Turbonuclease
  • Benzonase? Nuclease, ultrapure
  • Turbonuclease from Serratia marcescens
  • Nuclease from Phaseolus aureus sprouts from mung bean
  • Benzonase<sup>(R)</sup> Nuclease
  • Nuclease(Endonuclease from Serratia marcescens)
  • Nuclease, nucleateendo-
  • Endonuclease Ⅰ
  • Ultra Nuclease( GMP class )
  • Benz-Neburase
  • Binuclease
  • Binuclease Liquid
  • Binuclease, Benzonase Nuclease
  • Broad spectrum Nuclease
  • UltraNuclease
  • 9025-65-4
  • BioChemical
  • Application Index
  • Biochemicals and Reagents
  • Cell Lysis and Protein Extraction Reagents
  • Metabolomics
  • Nucleotide Metabolism
  • Protein Sample Clarification
  • Protein &amp; Nucleic Acid Isolation
  • Proteomics
  • Proteomics and Protein Expression
  • Sample Preparation
  • General Metabolic Enzymes
  • 3.1.x.x Acting on esters
  • 3.x.x.x Hydrolases
  • Enzymes, Inhibitors, and Substrates