4-NITROPHENYL PHOSPHATE DISODIUM SALT HEXAHYDRATE Chemical Properties

Melting point:

>300 °C(lit.)

storage temp. 

-20°C

solubility 

H₂O: 25 mg/mL at 20 °C, clear, yellow-green

form 

tablets

color 

white to pale yellow

PH

pH (50g/l, 25℃) : 8.0～9.0

Water Solubility 

almost transparency

BRN 

5324661

CAS DataBase Reference

333338-18-4(CAS DataBase Reference)

Safety Information

Safety Statements 

24/25

WGK Germany 

3

F 

8-10-21

HS Code 

29199000

MSDS

• Language:English Provider:SigmaAldrich
• Language:English Provider:ACROS

4-NITROPHENYL PHOSPHATE DISODIUM SALT HEXAHYDRATE Usage And Synthesis

Chemical Properties

White to off-white powder

Uses

Substrate for alkaline and acid phosphatase determinations4-Nitrophenyl phosphate disodium salt hexahydrate is a chromogenic substrate used in the determination of acid and alkaline phosphatases.

Uses

4-Nitrophenyl phosphate disodium salt hexahydrate has been used in:

• enzyme-linked immunosorbent assay (ELISA) to visualize glycoprotein VI (GPVI) binding to collagen type I (Col I)
• enzymatic color reaction for semi-quantitative measurement of allergen-specific IgE by ELISA
• alkaline phosphatase assay

Uses

Phosphatase substrate has been used in the chromogenic detection of adsorbed rabbit IgG to determine coating efficiency.

General Description

Chromogenic substrate for the determination of acid and alkaline phosphatases. The reaction produces a soluble end product that is yellow in color. The reaction may be stopped with 3 N NaOH and read at 405 nm.

Biochem/physiol Actions

4-Nitrophenyl phosphate disodium salt hexahydrate (pNPP) is a non-proteinaceous and non-specific substrate for determining protein tyrosine phosphatase activity. Hydrolysis of pNPP yields p-nitrophenol. pNPP is a common chromogenic substrate for alkaline phosphatase.

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