2-Iodoacetamide Chemical Properties

Melting point:

92-95 °C(lit.)

Boiling point:

297.1±23.0 °C(Predicted)

Density 

2.1103 (estimate)

storage temp. 

2-8°C

solubility 

Soluble in water, dimethyl formamide and ethanol. Slightly soluble in methanol.

form 

crystalline

pka

15.16±0.40(Predicted)

color 

White to pale yellow or beige

Water Solubility 

H2O: 0.5M at 20°C, clear, colorless

Sensitive 

Light Sensitive

BRN 

1739080

Stability:

Stable, but light sensitive. Incompatible with strong oxidizing agents, strong bases, reducing agents, acids.

InChIKey

PGLTVOMIXTUURA-UHFFFAOYSA-N

CAS DataBase Reference

144-48-9(CAS DataBase Reference)

NIST Chemistry Reference

Alpha-iodo acetamide(144-48-9)

EPA Substance Registry System

2-Iodoacetamide (144-48-9)

Safety Information

Hazard Codes 

T

Risk Statements 

25-42/43-43-36/37/38-53

Safety Statements 

22-36/37-45-37/39-26-24

RIDADR 

UN 2811 6.1/PG 3

WGK Germany 

3

RTECS 

AC4200000

F 

8-10-21

TSCA 

T

HazardClass 

6.1

PackingGroup 

III

HS Code 

29241900

MSDS

• Language:English Provider:2-Iodoacetamide
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2-Iodoacetamide Usage And Synthesis

Chemical Properties

White solid in amber, foil sealed microtubes. Soluble in hot water, easily soluble in ethanol.

Uses

2-Iodoacetamide is an alkylating reagent for cysteine residues in peptide sequencing. Its actions are similar to those of iodoacetate. By virtue of reaction with cysteine, it is an irreversible inhibitor of enzymes with cysteine at the active site. It reacts much more slowly with histidine residues, but that activity is responsible for inhibition of ribonuclease.

Application

Alkylating agent used in peptide mapping because it covalently binds with the thiols in cysteine to prevent disulfide bond formation 2-Iodoacetamide is used as an electrophile for covalent modification of nucleophilic residues on proteins such as cysteine, methionine and histidine. It is used to bind with thiol group of cysteine, thereby it protects the formation disulfide bonds. It is involved as an inhibitor of deubiquitinase enzymes (DUBs). Further, it acts as an alkylating sulfhydryl reagent.

Biological Activity

Iodoacetamide acts as an alkylating reagent for cysteine residues in peptide sequencing. It is an irreversible inhibitor of enzymes with cysteine at the active site. It reacts much more slowly with histidine residues, but that activity is responsible for inhibiting ribonuclease.

Synthesis

2-Iodoacetamide is synthesized by reacting chloroacetamide with sodium iodide. The chloroacetamide, anhydrous acetone, and anhydrous sodium iodide were refluxed on the bath for 15h. Cool to room temperature, filter out sodium chloride, recover acetone, pour into ice water of sodium bisulfate after a little cooling, and then neutralize to pH 6 with saturated sodium sulfate solution. Cool to crystallize and filter to obtain crude product. The crude product is recrystallized with water to obtain the finished product.

Purification Methods

Crystallise it from water or CCl4. It is used for tagging proteins. [Gurd Methods Enzymol 25 424 1972, Beilstein 2 IV 536.]

Preparation and handling

Alkylation Procedure
Iodoacetamide is unstable and light-sensitive. Prepare solutions immediately before use and perform alkylation in the dark. If iodoacetamide is present in limiting quantities and a slightly alkaline pH, cysteine modification will be the exclusive reaction. Excess iodoacetamide or non-buffered iodoacetamide reagent can also alkylate amines (lysine, N-termini), thioethers (methionine), imidazoles (histidine) and carboxylates (aspartate, glutamate).
1. Add 5 μl of 2% SDS and 45 μl of 200 mM ammonium bicarbonate (pH 8.0) to 20-100 μg of protein sample. Adjust volume to 100 μl with ultrapure water.
2. Add 5 μl of 200 mM Tris(2-carboxyethyl) phosphine hydrochloride (TCEP.HCl, Product No. 20490) and incubate sample at 55°C for 1 hour.
3. Immediately before use, dissolve one tube of iodoacetamide (9.3 mg) with 132 μl of 200 mM ammonium bicarbonate (pH 8.0) to make 375 mM iodoacetamide. Protect solution from light.
4. Add 5 μl of the 375 mM iodoacetamide to the sample and incubate for 30 minutes protected from light.
5. Proceed to proteolytic digestion before MS analysis or other processing.

Precautions

Store in a cool place. Light and moisture sensitive. Keep the container tightly closed in a dry and well-ventilated place. Incompatible with strong acids, strong bases, strong oxidizing agents and strong reducing agents.

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