H-MET-ALA-GLY-PRO-HIS-PRO-VAL-ILE-VAL-ILE-THR-GLY-PRO-HIS-GLU-GLU-OH Chemical Properties

Boiling point:

1982.4±65.0 °C(Predicted)

Density 

1.334±0.06 g/cm3(Predicted)

storage temp. 

-20°C

solubility 

DMSO: 15 mg/ml; Water: 10 mg/ml

form 

solid

pka

3.12±0.10(Predicted)

color 

White to off-white

Water Solubility 

Soluble to 2 mg/ml in water

Sequence

H-Met-Ala-Gly-Pro-His-Pro-Val-Ile-Val-Ile-Thr-Gly-Pro-His-Glu-Glu-OH

Safety Information

Safety Statements 

22-24/25

WGK Germany 

3

HS Code 

2933299090

MSDS

• Language:English Provider:SigmaAldrich

H-MET-ALA-GLY-PRO-HIS-PRO-VAL-ILE-VAL-ILE-THR-GLY-PRO-HIS-GLU-GLU-OH Usage And Synthesis

Description

NFAT inhibitor is a cell-permeable compound that selectively inhibits calcineurin-mediated dephosphorylation of NFAT. At 100 μM, this inhibitor effectively blocks calcineurin binding to NFAT without disrupting other calcineurin-dependent pathways, unlike the immunosuppressants cyclosporin A and FK506 which indiscriminately block all calcineurin phosphatase activity. NFAT inhibitor can disrupt NFAT-dependent expression of IL-2 and TNF-α when transfected in Jurak T cells and can prevent the activation and proliferation of T cells both in vitro (~ 43% at 1 μM using mixed lymphocyte cultures) and in vivo (10 mg/kg using C3H/HeN mice).

Uses

NFAT Inhibitor, VIVIT has been used to study the signaling pathways involved in controlling inhibitory molecules and subsequent onset of T-cell impairment.

Biochem/physiol Actions

High-affinity calcineurin-binding peptide that inhibits NFAT (Nuclear Factor of Activated T cells) activation and NFAT-dependent expression of endogenous cytokine genes in T cells.

storage

Store at -20°C

References

[1] JOSE? ARAMBURU. Affinity-Driven Peptide Selection of an NFAT Inhibitor More Selective Than Cyclosporin A[J]. Science, 1999, 285 5436. DOI: 10.1126/science.285.5436.2129
[2] MICHAEL H A ROEHRL. Selective inhibition of calcineurin-NFAT signaling by blocking protein-protein interaction with small organic molecules.[J]. Proceedings of the National Academy of Sciences of the United States of America, 2004, 101 20: 7554-7559. DOI: 10.1073/pnas.0401835101
[3] HAIXIANG YU. Therapeutic potential of a synthetic peptide inhibitor of nuclear factor of activated T cells as antirestenotic agent.[J]. Arteriosclerosis, Thrombosis, and Vascular Biology, 2006, 26 7: 1531-1537. DOI: 10.1161/01.atv.0000225286.30710.af
[4] XUEQIANG ZHAO. JNK1 negatively controls antifungal innate immunity by suppressing CD23 expression[J]. Nature Medicine, 2017, 23 3: 337-346. DOI: 10.1038/nm.4260

H-MET-ALA-GLY-PRO-HIS-PRO-VAL-ILE-VAL-ILE-THR-GLY-PRO-HIS-GLU-GLU-OH(249537-73-3)Related Product Information

• H-GLU-GLU-OH
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