Coumarin 343 X NHS ester Chemical Properties

Density 

1.41±0.1 g/cm3(Predicted)

solubility 

Soluble in DCM, DMSO, DMF

pka

12.88±0.20(Predicted)

Appearance

Yellow solid

ex/em

437/477 nm

ε(extinction coefficient)

39000 L⋅mol−1⋅cm−1

Φ(quantum yield)

0.63

InChI

InChI=1S/C26H29N3O7/c30-20-9-10-21(31)29(20)36-22(32)8-2-1-3-11-27-25(33)19-15-17-14-16-6-4-12-28-13-5-7-18(23(16)28)24(17)35-26(19)34/h14-15H,1-13H2,(H,27,33)

InChIKey

UTYUORAKIFFNAS-UHFFFAOYSA-N

SMILES

C(ON1C(=O)CCC1=O)(=O)CCCCCNC(C1=CC2=CC3=C4C(=C2OC1=O)CCCN4CCC3)=O

Coumarin 343 X NHS ester Usage And Synthesis

Description

Blue emitting Coumarin 343 dye can form a FRET pair with fluorescein (FAM). An amine reactive form, activated NHS ester._x000D_ This amine-reactive NHS ester contains an aminohexanoyl linker between the fluorophore, and the reactive group. This linker provides better solubility and spatial separation between the fluorophore, and the target molecule being labeled.

Chemical Properties

Appearance: Yellow solid
ex/em: 437/477 nm
Extinction coefficient (ε): 39000 L??mol??1??cm??1
Quantum yield (Φ): 0.63

Uses

Coumarin 343 X NHS ester is a homologue of Coumarin 343 (HY-W267364). Coumarin 343 is a hydrophilic fluorescent probe for use in microfluids.

Abs/Em Maxima

437/477 nm

Fluoresene quantum yield

0.63

Extinction Coefficient

39000

CF260

0.29

CF280

0.24

Coumarin 343 X NHS ester(946123-12-2)Related Product Information

• Coumarin 343 azide
• ATTO 390 NHS ESTER
• AMCA-NHS