Basic information Safety Supplier Related

FM4-64

Basic information Safety Supplier Related

FM4-64 Basic information

Product Name:
FM4-64
Synonyms:
  • Pyridinium, 4-[6-[4-(diethylamino)phenyl]- 1,3,5-hexatrienyl]-1-[3-(triethylammonio) propyl]-, dibromide
  • 4-64 is TM of Molecular Probes
  • FM®
  • N-(3-Triethylammoniopropyl)-4-(6-(4-(diethy
  • NeurotransRed C2 [N-(3-Triethylammoniumpropyl)-4-(6-(4-Diethylamino)phenyl)hexatrienyl)Pyridinium Dibromide], 
  • NEUROTRANSRED C2 [N-(3-TRIETHYLAMMONIUMPROPYL)-4-(6-(4-DIETHYLAMINO)PHENYL)HEXATRIENYL)PYRIDINIUM DIBROMIDE],  FM® 4-64 IS TM OF MOLECULAR PROBES;N-(3-TRIETHYLAMMONIOPROPYL)-4-(6-(4-(DIETHY
  • NeurotransRed C2 [N-(3-Triethylammoniumpropyl)-4-(6-(4-Diethylamino)phenyl)hexatrienyl)Pyridinium Dibromide], FM[R] 4-64 is TM of Molecular Probes
  • NEUROTRANSRED C2 [N-(3-TRIETHYLAMMONIUMPROPYL)-4-(6-(4-DIETHYLAMINO)PHENYL)HEXATRIENYL)PYRIDINIUM DIBROMIDE], FM[R] 4-64 IS TM OF MOLECULAR PROBES
CAS:
162112-35-8
MF:
C30H45BrN3+
MW:
527.61
Product Categories:
  • Styryl
Mol File:
162112-35-8.mol
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FM4-64 Chemical Properties

Melting point:
>200℃
storage temp. 
−20°C
solubility 
DMSO: soluble10mg/mL
form 
powder
color 
deep purple
Appearance
dark powder
Water Solubility 
water: 10mg/mL
DMSO: soluble
methanol: soluble
λmax
505nm
Biological Applications
Detecting FRET in cells; monitoring fast neuronal activity and signaling; quantifying plasma membrane expression; cytotoxicity assay; membrane fusion assay; probe for endocytosis; probe for exocytosis
InChIKey
HMPYRCUBZVFZMV-UHFFFAOYSA-M
SMILES
N(C1C=CC(C=CC=CC=CC2=CC=[N+](CCC[N+](CC)(CC)CC)C=C2)=CC=1)(CC)CC.[Br-]
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Safety Information

Safety Statements 
22-24/25
WGK Germany 
3
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FM4-64 Usage And Synthesis

Description

SynaptoProbe® Red is an analog of FM®4-64, a widely used red fluorescent dye for studying synaptic activity in the synapses or neuromuscular junctions by staining synaptic vesicles.


This water-soluble and non-toxic to cells dye is nonfluorescent in an aqueous medium but becomes highly fluorescent after embedding into the outer leaflet of the cell membrane. When a neuron actively releases neurotransmitters, the dye becomes internalized within the recycled synaptic vesicles and stains the nerve terminals.


SynaptoProbe® Red can be used to visualize synaptic vesicles and their exocytosis and endocytosis in living neurons, identify actively firing neurons, and investigate the mechanisms of activity-dependent vesicle cycling. It is also helpful for the visualization of shear stress-induced plasma membrane damage in fibroblasts.

Uses

Water Soluble Fluorescent RH probe

Uses

N-(3-Triethylammoniopropyl)-4-(6-(4-(diethylamino)phenyl) hexatrienyl)pyridinium dibromide is a styryl dyes or a fluorescent dye. Dyes and metabolites.

Biological Activity

Fluorescent dye suitable for monitoring synaptic activity at neuromuscular junctions.

storage

Store at -20°C

Abs/Em Maxima

536/736 nm

FM4-64Supplier

Shanghai Annengjian Biomedical Technology Co., LTD Gold
Tel
021-68781987 13818305065
Email
sales@energybiopharma.com
Shanghai Aladdin Bio-Chem Technology Co.,LTD
Tel
400-6206333 13167063860
Email
anhua.mao@aladdin-e.com
Shanghai Macklin Biochemical Co.,Ltd.
Tel
15221275939
Email
shenlinxing@macklin.cn
Shanghai YuanYe Biotechnology Co., Ltd.
Tel
021-61312847; 18021002903
Email
3008007409@qq.com
Chengdu Huachun Technology Co., Ltd
Tel
400-1166-196 15982826727
Email
cdhxsj@163.com
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FM4-64(162112-35-8)Related Product Information