Basic information Description References Safety Supplier Related
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Methyl Green zinc chloride salt

Basic information Description References Safety Supplier Related

Methyl Green zinc chloride salt Basic information

Product Name:
Methyl Green zinc chloride salt
Synonyms:
  • METHYL GREEN, ZINC CHLORIDE
  • METHYL GREEN, ZINC CHLORIDE SALT
  • LIGHT GREEN B
  • DOUBLE GREEN SF
  • CI NO 42590
  • CI 42590
  • ETHYL GREEN ZINC CHLORIDE SALT
  • Methyl green, crystal violet free, (
CAS:
7114-03-6
MF:
C27H35BrCl3N3Zn
MW:
653.23
EINECS:
230-415-4
Product Categories:
  • Triphenylmethane
Mol File:
7114-03-6.mol
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Methyl Green zinc chloride salt Chemical Properties

Melting point:
>300 °C(lit.)
storage temp. 
Store at RT.
solubility 
Soluble in water, ethanol; insoluble in xylene
form 
Solid
color 
Green
PH Range
Yellow (0.1) to greenish-blue (2.3)
Water Solubility 
70 g/L (20 ºC)
λmax
629 nm, 423 nm
Stability:
Stable. Incompatible with strong oxidizing agents.
Biological Applications
Detecting phosphates,polyphosphates,tocopherol,tocopherol acetate
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Safety Information

Hazard Codes 
Xi,N,C
Risk Statements 
36/37/38-50/53-34-22
Safety Statements 
26-36-61-60-45-36/37/39
WGK Germany 
3
HS Code 
29215900

MSDS

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Methyl Green zinc chloride salt Usage And Synthesis

Description

Methyl Green, Zinc chloride salt (chemical formula: C27H35Cl4N3Zn) is the zinc chloride salt form of Methyl green. Methyl green is a cationic or positive charged stain. Since it can binds selectively to the AT-rich regions of native DNA, it is mainly used for the DNA and RNA staining in the cell nuclei. Methyl Green, Zinc chloride Salt, as a CERTISTAIN® dry dye for DNA staining, it has many application: used as a tracking dye in acid buffers for electrophoresis; used to demonstrate DNA and RNA in tissues sections in the Methyl Green-Pyronin method; used as an exclusion viability stain for cells. In addition, it can also be used in fluorimetric assay and flow cytometry.

References

http://www.sigmaaldrich.com/catalog/product/sigma/67060?lang=zh&region=CN
https://pubchem.ncbi.nlm.nih.gov/compound/3084002#section=Canonical-SMILES
https://en.wikipedia.org/wiki/Methyl_green
http://www.abcam.com/methyl-green-zinc-chloride-salt-ab146287.html

Chemical Properties

maroon to reddish-brown crystals

Uses

Methyl Green is a DNA stain used as a tracking dye in acid buffers for electrophoresis. It helps differentiate bacteria and is also a tissue stain for nucleoli and mitochondria in tissues. It is used with Bismarck Brown frequently.

Purification Methods

Crystallise the dye from hot water. [Beilstein 13 IV 2286.]

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