Methyl Green zinc chloride salt Chemical Properties

Melting point:

>300 °C(lit.)

bulk density

300kg/m3

storage temp. 

Store at RT.

solubility 

Soluble in water, ethanol; insoluble in xylene

Colour Index 

42590

form 

Solid

color 

Green

PH Range

Yellow (0.1) to greenish-blue (2.3)

PH

4.6 (20°C, 10g/L in H2O)

Water Solubility 

70 g/L (20 ºC)

λmax

629 nm, 423 nm

ε(extinction coefficient)

≥14000 at 311-317 in water at 0.005g/L
≥50000 at 629-635 in water at 0.005g/L
≥9000 at 420-426 in water at 0.005g/L

Stability:

Stable. Incompatible with strong oxidizing agents.

Biological Applications

Detecting phosphates,polyphosphates,tocopherol,tocopherol acetate

Safety Information

Hazard Codes 

Xi,N,C

Risk Statements 

36/37/38-50/53-34-22

Safety Statements 

26-36-61-60-45-36/37/39

WGK Germany 

3

HS Code 

29215900

MSDS

• Language:English Provider:SigmaAldrich

Methyl Green zinc chloride salt Usage And Synthesis

Description

Methyl Green, Zinc chloride salt (chemical formula: C27H35Cl4N3Zn) is the zinc chloride salt form of Methyl green. Methyl green is a cationic or positive charged stain. Since it can binds selectively to the AT-rich regions of native DNA, it is mainly used for the DNA and RNA staining in the cell nuclei. Methyl Green, Zinc chloride Salt, as a CERTISTAIN® dry dye for DNA staining, it has many application: used as a tracking dye in acid buffers for electrophoresis; used to demonstrate DNA and RNA in tissues sections in the Methyl Green-Pyronin method; used as an exclusion viability stain for cells. In addition, it can also be used in fluorimetric assay and flow cytometry.

References

http://www.sigmaaldrich.com/catalog/product/sigma/67060?lang=zh&region=CN
https://pubchem.ncbi.nlm.nih.gov/compound/3084002#section=Canonical-SMILES
https://en.wikipedia.org/wiki/Methyl_green
http://www.abcam.com/methyl-green-zinc-chloride-salt-ab146287.html

Chemical Properties

maroon to reddish-brown crystals

Uses

Methyl Green is a DNA stain used as a tracking dye in acid buffers for electrophoresis. It helps differentiate bacteria and is also a tissue stain for nucleoli and mitochondria in tissues. It is used with Bismarck Brown frequently.

Biological Activity

Marker dye for DNA in acid and neutral media.
Methyl green is mainly used as a DNA stain in histochemistry. at low concentrations it binds selectively to AT-rich regions of native DNA. However, at high concentrations it is present at all available DNA sites.

Purification Methods

Crystallise the dye from hot water. [Beilstein 13 IV 2286.]

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