DHR 6G [DihydrorhodaMine 6G] Chemical Properties

solubility 

Soluble in N,N-dimethylformamide, dimethyl sulfoxide, methanol

form 

Solid

pka

4.97 ± 0.40, most basic, temperature: 25 °C

color 

Light pink solid

λmax

296 nm

Biological Applications

Detecting genetic anomalies,methylation in DNA,intracellular hydrogen peroxide in tumor cells,intracellular active oxygen and reduced glutathione,gene expression,7reactive oxygen species (ROS),small RNAs; nucleic acid ligation assays

Major Application

Light-transmitting materials/construction boards

Safety Information

HS Code 

2932.99.7000

DHR 6G [DihydrorhodaMine 6G] Usage And Synthesis

Uses

Dihydrorhodamine 6G (DHR 6G) is the reduced form of Rhodamine 6G, which is used as fluorescent mitochondrial dye. It is nonfluorescent, but it readily enters most of the cells and is oxidized by oxidative species or by cellular redox systems to the fluorescent rhodamine 6G that accumulates in mitochondrial membranes. Dihydrorhodamine 6G is useful for detecting reactive oxygen species (ROS) including superoxide[1].

References

[1] Yong Qin, et al. Dihydrorhodamine 123 is superior to 2,7-dichlorodihydrofluorescein diacetate and dihydrorhodamine 6G in detecting intracellular hydrogen peroxide in tumor cells. Cell Biol Int. 2008 Feb;32(2):224-8. DOI:10.1016/j.cellbi.2007.08.028

DHR 6G [DihydrorhodaMine 6G](217176-83-5)Related Product Information

• DIHYDROETHIDIUM
• DIHYDRORHODAMINE 123