DIHYDRORHODAMINE 123 Chemical Properties

Melting point:

163–165℃

Boiling point:

526.9±50.0 °C(Predicted)

Density 

1.313±0.06 g/cm3(Predicted)

storage temp. 

-20°C

solubility 

DMSO: >5mg/mL

form 

powder

pka

4.70 ± 0.40, most basic, temperature: 25 °C

color 

Light red or pinkish-white

λmax

289 nm

BRN 

5989965

Biological Applications

Generating,detecting & measuring reactive oxygen species; generating,detecting & measuring reactive nitrogen species; detecting phosphates,peroxynitrile; measuring respiratory burst; chronic granulomatous disease DHR assay; screening

Safety Information

Safety Statements 

22-24/25

WGK Germany 

3

F 

10-23

MSDS

• Language:English Provider:SigmaAldrich

DIHYDRORHODAMINE 123 Usage And Synthesis

Description

DHR is a cell-permeable fluorogenic probe that is used as an indicator of intracellular peroxynitrite formation. It is oxidized by peroxynitrite to the highly fluorescent product rhodamine in vitro. Neither nitric oxide, superoxide, nor hydrogen peroxide alone appear to oxidize DHR. Formation of rhodamine can be monitored by fluorescence spectroscopy using excitation and emission wavelengths of 500 and 536 nm, respectively, or by absorbance spectroscopy at 500 nm (ε = 78,800 M⁻¹cm⁻¹). DHR has been used to investigate reactive oxygen intermediates produced by endothelial cells, eosinophils, and reactive microglia.

Uses

Dihydrorhodamine 123 is the uncharged and nonfluorescent reduction product of the mitochondrion-selective dye rhodamine 123 used for investigation of reacitve oxygen intermediates.

Uses

Dihydrorhodamine 123 (DHR) has been used for the detection of free intracellular radicals or ROS (reactive oxygen species) in head kidney-derived macrophages. It has also been used in DHR assay in red blood cells and in oxidative burst assay in neutrophils.

General Description

Cell-permeable fluorogenic probe that is useful for the detection of reactive oxygen species (ROS) such as peroxide and peroxynitrite. Dihydrorhodamine is not fluorescent unless it is converted to rhodamine 123.

Biochem/physiol Actions

Cell permeable: yes

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