TRYPSINOGEN Chemical Properties

storage temp. 

2-8°C

form 

essentially salt-free, lyophilized powder

Safety Information

Hazard Codes 

Xn

Risk Statements 

36/37/38-42

Safety Statements 

22-24-26-36/37

WGK Germany 

3

F 

10-21

MSDS

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TRYPSINOGEN Usage And Synthesis

Uses

Trypsinogen from bovine pancreas is suitable for use in:

• the secondary structure analysis of proteins in H2O solution using single-pass attenuated total reflection Fourier transform infrared (ATR-FT-IR) microscopy
• tuning and calibration of electrospray ionization quadrupole time-of-flight (ESI-Q-TOF) mass spectrometer
• the secondary structure analysis of proteins by infrared (IR) spectroscopy
• SDS-PAGE as a molecular weight standard (24kDa)

General Description

Trypsinogen is a proenzyme (zymogen) that is activated to form trypsin. It is synthesized in the pancreas and activated by enterokinase once it reaches the lumen of the small intestine. Bovine trypsinogen is a single polypeptide chain of 229 amino acids that is cross linked by six disulfide bridges. Enterokinase cleaves a hexapeptide to from the NH₂ terminus of trypsinogen at the Lys6 - Ile7 peptide bond and activates it. Trypsin, thus formed, autocatalytically activates more trypsinogen to trypsin. This native form of trypsin is called β-trypsin, which undergoes autolysis at Lys₁₃₁ - Ser₁₃₂ resulting in α-trypsin that is held together by disulfide bridges. Trypsin is a serine protease with His₄₆ and Ser₁₈₃ at the active site. The pH optimum of trypsin is 7 - 9.

Biochem/physiol Actions

Hereditary pancreatitis was shown to be caused by a Arg-His substitution at residue 117 of trypsinogen causing auto-activation of trypsinogen to trypsin.

TRYPSINOGEN(9002-08-8)Related Product Information

• Trypsin inhibitor
• Basic protease
• Trypsin
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• TRYPSINOGEN
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• H-ASP-LYS-OH
• ANTI-TRYPSINOGEN, BOVINE PANCREAS,ANTI-TRYPSINOGEN
• N-acetyl-trypsinogen
• TRYPSINOGEN (POTENTIAL ACTIVITY)
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• trypsinogen activation peptide
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• TRYPSINOGEN INHIBITOR EX SOYBEAN
• ANTI-TRYPSIN/TRYPSINOGEN
• ANTI-TRYPSINOGEN, BOVINE PANCREAS HORSERADISH PEROXIDASE CONJUGATED
• ANTI-TRYPSINOGEN, BIOTIN