Z-D-ARG-GLY-ARG-PNA 2 HCL Chemical Properties

storage temp. 

Store at -20°C

solubility 

DMF: 14 mg/ml; DMSO: 14 mg/ml; Ethanol: partially soluble; PBS (pH 7.2): 0.5 mg/ml

form 

A solid

Stability:

Hygroscopic

Z-D-ARG-GLY-ARG-PNA 2 HCL Usage And Synthesis

Description

Z-D-Arg-Gly-Arg-pNA is a colorimetric substrate for Factor Xa.^1,2 Factor Xa preferentially binds to and cleaves the Arg-Gly-Arg (RGR) peptide sequence to release p-nitroanilide (pNA), which can be quantified by colorimetric detection at 405 nm as a measure of Factor Xa activity.

Uses

Z-D-Arg-Gly-Arg-pNA Dihydrochloride is a reagent used in the preparation and testing of tripeptides and intermediates (arginine-containing) as reagent for the determination of serine protease.

References

1. Gustavsson, S.I., and Arielly, S. New Peptide Derivatives ,(1989).
2. Joo, H.-S., Park, G.-C., Kim, K.-M., et al. Novel alkaline protease from the polychaeta, Periserrula leucophryna: Purification and characterization Proc. Biochem. 36(8-9),893-900(2001).

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