DBCO-S-S-SE
DBCO-S-S-SE Basic information
- Product Name:
- DBCO-S-S-SE
- Synonyms:
-
- DBCO-S-S-NHS ester
- DBCO-S-S-SE
- DBCO-S-S-succinimidyl ester
- Dibenzocyclooctyne-S-S-N-hydroxysuccinimidyl ester
- MW:
- 0
- Mol File:
- Mol File
DBCO-S-S-SE Chemical Properties
- Melting point:
- 192-197 °C
- storage temp.
- -20°C
- form
- solid
DBCO-S-S-SE Usage And Synthesis
Uses
Cleavable succinimidyl ester/NHS functionalized cyclooctyne derivative for incorporation of the cyclooctyne moiety into amine containing compounds or biomolecules. Cyclooctynes are useful in strain-promoted copper-free azide-alkyne cycloaddition reactions. This dibenzocyclooctyne will react with azide functionalized compounds or biomolecules without the need for a Cu(I) catalyst to result in a stable triazole linkage. The disulfide present can be readily cleaved using mild reducing conditions (10-50 mM DTT at 37°C for 30 min or 5% ?-mercaptoethanol in SDS-PAGE sample buffer at 100°C for 5 minutes).
Caution: Please avoid reducing agents and amine or azide containing buffers during coupling/labeling reactions. Only use reducing agents when cleavage of the disulfide is desired.
reaction suitability
reaction type: click chemistry
reagent type: cross-linking reagent