Mouse Vascular Endothelial Growth Factor-164 (mVEGF164 ) Usage And Synthesis

Description

The bioactivity of recombinant mVEGF₁₆₄ was determined in a cell proliferation assay using HUVEC. The ED₅₀ of each lot is between 1-5 ng/ml.

Source

Human 293 Cells

Background

VEGF164 is one of many splice variants of the mouse VEGF-A gene, and is one amino acid shorter than its human counterpart, VEGF165. VEGF164 is produced by a number of cells including endothelial cells, macrophages and T cells. VEGF164 is involved in angiogenesis, vascular endothelial cell survival, growth, migration and vascular permeability. Gene expression is induced by hypoxia, inflammatory cytokines and oncogenes. VEGF164 binds to heparan sulfate and is retained on the cell surface and in the extracellular matrix. VEGFR1 and VEGFR2 are the receptor tyrosine kinases for VEGF164. NRP-1 and NRP-2 may function as co-receptors and enhance VEGFR2 signaling. Binding of VEGF164 to VEGFR1 and VEGR2 leads to activation of the PI3K/AKT, p38 MAPK, FAK and Paxillin.

References

[1] Haigh, J.J. (2008) Organogenesis 4, 247-56.
[2] Takahashi, H. and Shibuya, M. (2005) Clin Sci (Lond) 109, 227-41.
[3] Neufeld, G. et al. (1999) FASEB J 13, 9-22.