QUIN 2-AM Property

Density 

1.360

Flash point:

113 °C

storage temp. 

−20°C

solubility 

DMSO: soluble

form 

A solid

λmax

348 nm

Biological Applications

Calcium indicator

Safety

Hazard Codes 

Xi

Risk Statements 

36/37/38

Safety Statements 

26-36

WGK Germany 

3

F 

1-8-10

Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Warning

Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

Precautionary statements

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P302+P352IF ON SKIN: wash with plenty of soap and water.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

P321Specific treatment (see … on this label).

P332+P313IF SKIN irritation occurs: Get medical advice/attention.

P362Take off contaminated clothing and wash before reuse.

QUIN 2-AM Chemical Properties,Usage,Production

Description

Quin-2 AM is a cell-permeant acetoxymethyl ester of the high affinity fluorescent calcium indicator quin-2 . As quin-2 AM enters cells, it is hydrolyzed by intracellular esterases to produce quin-2. Quin-2 binds calcium with a K_d value of 115 nM. It displays high selectivity for calcium, as it is not affected by sodium gradients, membrane potential, or intracellular pH. High affinity probes like quin-2 are ideal for monitoring low levels of calcium, as are found in resting cells. Loadings of up to 2 mM quin-2 are without serious toxic effects, so quin-2 may be used to buffer intracellular calcium transients. Excitation and emission maxima for quin-2 are 339 and 492 nm, respectively.

Uses

Fluorescent probe for measurement of calcium in biological systems and for selected divalent cations.

Uses

2-[(2-Amino-5-methylphenoxy)methyl]-6-methoxy-8-aminoquinoline-N,N,N',N'-tetraacetic acid tetrakis(acetoxymethyl ester)

Uses

Quin 2-AM is a cell permeable fluorescent probe for Ca2+ that gives a large shift in the UV absorption spectrum and a 20-fold increase in fluorescence upon Ca2+ binding.