MOPS Property

Melting point:

277-282 °C

Density 

1.3168 (rough estimate)

vapor pressure 

0Pa at 25℃

refractive index 

1.6370 (estimate)

Flash point:

116 °C

storage temp. 

room temp

solubility 

H₂O: 1 M at 20 °C, clear

form 

Powder/Solid

color 

White

PH

2.5-4.0 (25℃, 1M in H2O)

pka

7.2(at 25℃)

PH Range

6.5 - 7.9

Odor

Odorless

Water Solubility 

1000 g/L (20 ºC)

λmax

λ: 260 nm Amax: 0.020
λ: 280 nm Amax: 0.015

Merck 

14,6265

BRN 

1106776

Stability:

Stable. Incompatible with strong bases, strong oxidizing agents.

InChIKey

DVLFYONBTKHTER-UHFFFAOYSA-N

LogP

-2.94 at 20℃

CAS DataBase Reference

1132-61-2(CAS DataBase Reference)

EPA Substance Registry System

4-Morpholinepropanesulfonic acid (1132-61-2)

Safety

Hazard Codes 

Xi

Risk Statements 

36/37/38

Safety Statements 

26-36

WGK Germany 

1

RTECS 

QE9104530

TSCA 

Yes

HS Code 

29349990

Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Warning

Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P304+P340IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

P405Store locked up.

MOPS Chemical Properties,Usage,Production

Description

MOPS (3-morpholinopropanesulfonic acid) is a buffer introduced by Good et al. in the 1960s. It is a structural analog to MES. Its chemical structure contains a morpholine ring. HEPES is a similar pH buffering compound that contains a piperazine ring. With a pKa of 7.20, MOPS is an excellent buffer for many biological systems at near-neutral pH.It is used as a synthetic buffering agent below pH 7.5.

application

MOPS is frequently used as a buffering agent in biology and biochemistry. It has been tested and recommended for polyacrylamide gel electrophoresis. Usage above 20 mM in mammalian cell culture work is not recommended. MOPS buffer solutions become discolored (yellow) over time, but reportedly slight discoloration does not significantly affect the buffering characteristics.

Reference

P. H. Quail, D. Marme, E. Schäfer, Particle-bound phytochrome from maize and pumpkin, Nature New Biology, 1973, vol. 245, pp. 189-191

Chemical Properties

White/clear crystalline powder

Uses

MOPS acts as a multi-purpose buffering agent used in various biological research.

Uses

Due to its inert nature, 3-morpholinopropanesulfonic acid (MOPS) is widely used as a buffer in many biochemical studies. MOPS has been used as a cell culture additive component in lentiviral particle production,  a buffering agent in microbial growth medium and nuclei extraction buffer, a component of Roswell Park Memorial Institute (RPMI) medium for diluting fungal inoculum, and a buffer in capillary-zone electrophoresis to test performance. It is also used for the dilution of proteins from algal samples.

Definition

ChEBI: 3-(N-morpholino)propanesulfonic acid is a Good's buffer substance, pKa = 7.2 at 20 ℃. It is a member of morpholines, a MOPS and an organosulfonic acid. It is a conjugate acid of a 3-(N-morpholino)propanesulfonate. It is a tautomer of a 3-(N-morpholiniumyl)propanesulfonate.

General Description

3-(N-Morpholino)propane sulfonic acid (MOPS) is an N-substituted amino sulfonic acid with a morpholinic ring. MOPS is capable of buffering within a pH range of 6.5-7.9. MOPS is widely used in biological and biochemical studies due to its inert properties. It does not interact with any metal ions in solutions and has significant metal-buffer stability especially with copper (Cu), nickel (Ni), manganese (Mn), zinc (Zn), cobalt (Co) ions. MOPS buffer maintains the pH of the mammalian cell culture medium. MOPS functions to maintain pH in denaturing gel electrophoresis of RNA. MOPS can modify lipid interactions and influence the thickness and barrier properties of membranes. MOPS interacts with bovine serum albumin and stabilizes the protein. Hydrogen peroxide oxidizes MOPS slowly to N-oxide form.

Flammability and Explosibility

Not classified

Synthesis

Morpholine and 1,3-Propanesultone are added to absolute ethanol for reaction. The crude product synthesized is purified to obtain 3-Morpholinopropanesulfonic acid.