5-ETHYNYL-2'-DEOXYURIDINE
- Product Name
- 5-ETHYNYL-2'-DEOXYURIDINE
- CAS No.
- 61135-33-9
- Chemical Name
- 5-ETHYNYL-2'-DEOXYURIDINE
- Synonyms
- abs9124;EYdU;5-E-dU;Uridine,2;Aids072372;Aids-072372;5-Ethynyl-dU;-deoxyuridine;5-Ethynyl-durd;5-Ethynyl-2'-dU
- CBNumber
- CB0243359
- Molecular Formula
- C11H12N2O5
- Formula Weight
- 252.22
- MOL File
- 61135-33-9.mol
5-ETHYNYL-2'-DEOXYURIDINE Property
- Melting point:
- 199°C(lit.)
- Density
- 1.55±0.1 g/cm3(Predicted)
- storage temp.
- -20°C
- solubility
- Soluble in DMSO (up to 10 mg/ml).
- pka
- 8.29±0.10(Predicted)
- form
- powder
- color
- White or off-white
- Stability:
- Stable for 1 year from date of purchase as supplied. Solutions in DMSO may be stored at -20° for up to 2 months.
- InChIKey
- CDEURGJCGCHYFH-DJLDLDEBSA-N
- CAS DataBase Reference
- 61135-33-9
Safety
- Hazard Codes
- T
- Risk Statements
- 46-61
- Safety Statements
- 53-45-24/25
- RTECS
- YU7510000
- HS Code
- 29349990
Hazard and Precautionary Statements (GHS)
- Symbol(GHS)
-
- Signal word
- Danger
- Hazard statements
-
H340May cause genetic defects
H361Suspected of damaging fertility or the unborn child
- Precautionary statements
-
P201Obtain special instructions before use.
P308+P313IF exposed or concerned: Get medical advice/attention.
N-Bromosuccinimide Price
- Product number
- 900584
- Product name
- 5-Ethynyl-2′-deoxyuridine
- Purity
- 95%
- Packaging
- 50mg
- Price
- $147
- Updated
- 2024/03/01
- Product number
- 900584
- Product name
- 5-Ethynyl-2′-deoxyuridine
- Purity
- 95%
- Packaging
- 500mg
- Price
- $1030
- Updated
- 2024/03/01
- Product number
- E1057
- Product name
- 5-Ethynyl-2'-deoxyuridine
- Purity
- >98.0%(HPLC)(T)
- Packaging
- 50mg
- Price
- $89
- Updated
- 2024/03/01
- Product number
- E1057
- Product name
- 5-Ethynyl-2'-deoxyuridine
- Purity
- >98.0%(HPLC)(T)
- Packaging
- 200mg
- Price
- $258
- Updated
- 2024/03/01
- Product number
- 20518
- Product name
- 5-Ethynyl-2'-deoxyuridine
- Purity
- ≥98%
- Packaging
- 50mg
- Price
- $44
- Updated
- 2024/03/01
5-ETHYNYL-2'-DEOXYURIDINE Chemical Properties,Usage,Production
Description
5-Ethynyl-2′-deoxyuridine (61135-33-9) is a novel agent for fast and sensitive detection of DNA synthesis?in vivo.1 5-EdU is incorporated into proliferating cells followed by detection with a fluorescent azide using click ligation.1 In contrast to the use of BrdU, the new method does not require sample fixation or DNA denaturation. 5-Ethynyl-2′-deoxyuridine may be used for studying cell proliferation in the central nervous system and may be combined with BrdU staining in a novel protocol allowing for double labeling of DNA synthesis.2
Uses
5-Ethynyl-2’deoxyuridine is a extensively used nucleoside analog used as a probe for DNA synthesis. However, 5-Ethynyl-2’deoxyuridine is a toxic anti-metabolite, which potentially causes DNA instabi liy, necrosis and cell-cycle arrest, therefore it is often used for short-term DNA synthesis in tissue culture and living organisms where prolonged cell survival is not required.
Uses
5-Ethynyl-2′-deoxyuridine (EdU) is a thymidine analog that can be incorporated into replicating DNA for detection of cell proliferation. It has an alkyne handle that can ligate with azide-containing fluorescent probes through a highly efficient click chemistry reaction. EdU nucleoside can be incorporated into the DNA of cells either in culture or in animals without showing significant toxicity. The assay offers a simpler workflow for detecting cell proliferation than anti-BrdU assays.
For information on Baseclick kits containing all the reagents for the assays see: Baseclick kits
For a listing of the Baseclick kits see: Baseclick kits
storage
Store at -20°C
References
1) Salic and Mitchison (2008),?A chemical method for fast and sensitive detection of DNA synthesis in vivo; Proc. Natl. Acad. Sci. USA,?105?2415 2) Zeng?et al. (2010),?Evaluation of 5-ethynyl-2′-deoxyuridine staining as a sensitive and reliable method for studying cell proliferation in the adult nervous system; Brain Res.,?1319C?21
5-ETHYNYL-2'-DEOXYURIDINE Preparation Products And Raw materials
Raw materials
Preparation Products
5-ETHYNYL-2'-DEOXYURIDINE Suppliers
- Tel
- 03-36680489
- Fax
- 03-3668-0520
- Sales-JP@TCIchemicals.com
- Country
- Japan
- ProdList
- 28387
- Advantage
- 80
View Lastest Price from 5-ETHYNYL-2'-DEOXYURIDINE manufacturers
- Product
- 5-Ethynyl-2'-deoxyuridine 61135-33-9
- Price
- US $0.00-0.00/g
- Min. Order
- 10g
- Purity
- 98%min
- Supply Ability
- 1000g
- Release date
- 2021-09-03
- Product
- 5-Ethynyl-2'-deoxyuridine 61135-33-9
- Price
- US $0.00/g
- Min. Order
- 5g
- Purity
- >95.00%
- Supply Ability
- 5g
- Release date
- 2024-03-30
- Product
- 5-ETHYNYL-2'-DEOXYURIDINE 61135-33-9
- Price
- US $0.00-0.00/kg
- Min. Order
- 1kg
- Purity
- 98%
- Supply Ability
- 1Ton
- Release date
- 2022-09-27