Uridine

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Overview Biosynthesis and source Applications Reference

Product Name: Uridine
CAS No.: 58-96-8
Chemical Name: Uridine
Synonyms: Uridine;1-((2R,3R,4S,5R)-3,4-dihydroxy-5-(hydroxyMethyl)tetrahydrofuran-2-yl)pyriMidine-2,4(1H,3H)-dione;URD;UR;Uridin;URACIL-1-BETA-D-RIBOFURANOSIDE;D-URIDINE;β-Uridine;NFDB2;Uridine
CBNumber: CB0330400
Molecular Formula: C9H12N2O6
Formula Weight: 244.2
MOL File: 58-96-8.mol

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Uridine Property

Melting point:: 163-167 °C(lit.)
alpha: 8.4 º (c=2,water)
Boiling point:: 387.12°C (rough estimate)
Density: 1.4221 (rough estimate)
refractive index: 9 ° (C=2, H2O)
storage temp.: 2-8°C
solubility: H₂O: 50 mg/mL
form: powder
pka: 9.39±0.10(Predicted)
color: White to almost white
PH: 9.05
Water Solubility: Soluble in water, dimethylsulfoxide, and methanol.
λmax: 262 (pH 7)；262 (pH 12)
Merck: 14,9877
BRN: 754904
InChIKey: DRTQHJPVMGBUCF-XVFCMESISA-N
LogP: -1.581 (est)
CAS DataBase Reference: 58-96-8(CAS DataBase Reference)
NIST Chemistry Reference: Uridine(58-96-8)
EPA Substance Registry System: Uridine (58-96-8)

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Safety

Hazard Codes: Xi
Risk Statements: 36/37/38
Safety Statements: 24/25-36-26
WGK Germany: 3
RTECS: YR1450000
F: 10
Hazard Note: Keep Cold
TSCA: Yes
HS Code: 29335990

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Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Warning

Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P271Use only outdoors or in a well-ventilated area.

P280Wear protective gloves/protective clothing/eye protection/face protection.

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N-Bromosuccinimide Price

Sigma-Aldrich

Product number: U3003
Product name: Uridine
Purity: powder, BioReagent, suitable for cell culture
Packaging: 5g
Price: $68.6
Updated: 2024/03/01

Sigma-Aldrich

Product number: PHR2244
Product name: Uridine
Purity: PharmaceuticalSecondaryStandard;CertifiedReferenceMaterial
Packaging: 100mg
Price: $553
Updated: 2024/03/01

TCI Chemical

Product number: U0020
Product name: Uridine
Purity: >98.0%(HPLC)(T)
Packaging: 5g
Price: $23
Updated: 2024/03/01

TCI Chemical

Product number: U0020
Product name: Uridine
Purity: >98.0%(HPLC)(T)
Packaging: 25g
Price: $66
Updated: 2024/03/01

Alfa Aesar

Product number: A15227
Product name: Uridine
Purity: 99%
Packaging: 5g
Price: $39.2
Updated: 2024/03/01

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Uridine Chemical Properties,Usage,Production

Overview

Uridine is one of the key nucleotide that making RNA^[1-3]. It is a glycosylated pyrimidine-analog containing uracil attached to a ribose ring[or more specifically, a ribofuranose] via a β-N1-glycosidic bond. It is one of the five standard nucleosides which make up nucleic acids[including both RNA and DNA] with the others four being adenosine, thymidine, cytidine and guanosine. The five nucleosides are commonly abbreviated to their one-letter codes U, A, T, C and G respectively. Thymidine is found in deoxyribonucleic acid[DNA] and not ribonucleic acid(RNA]. Conversely, uridine is found in RNA and not DNA^{[1, 3]}. The remaining three nucleosides can be found in both RNA and DNA. In RNA, they would be represented as A, C and G whereas in DNA they would be represented as dA, dC and dG^[1,3].

Biosynthesis and source

Uridine is widely produced in the form of uridine monophosphate[uridylate] through the decarboxylation of orotidylate, being catalyzed by orotidylate decarboxylase^[4]. The orotidylate is produced from orotate, which is combined with 5-phosphoribosyl-1-pyrophosphate[PRPP] to form orotidylate by pyrimidine phosphoribosyltransferase. PRPP is created from ribose-5-phosphate by a further phosphorylation, serving as an energetic molecule to drive the reaction forward, while orotate is generated in several steps from carbamoyl phosphate and aspartate^[4].
Diet is not an important source of uridine. Clinical studies and animal experimentation suggest that the
liver synthesizes and degrades uridine, and is likely to have a central role in maintaining plasma uridine. Blood platelets and storage organelles of various species are reported to contain UTP and may provide releasable pools of uridine after catabolism^[5].

Applications

Uridine is phosphorylated to nucleotides, which are used for DNA and RNA synthesis as well as for the synthesis of membrane constituents and glycosylation^[6-8]. Uridine plays a very important role in the glycolysis pathway of galactose. It can be used as a precursor in the production of CDP-choline. It is an important nutrient and widely used as a dietary supplement. It can improve the brain cholinergic functions and hepatic mitochondrial function in certain liver toxins. It plays a major role in pain physiology and brain energy utilization to maintain ATP production under restricted oxygen conditions^{[6, 8]}. Uridine has many biological effects and, is thus can be used for the treatment of various kinds of diseases. In general, uridine can be used for the treatment for the following diseases such as cardiovascular disease and hypertension, respiratory dysfunction, liver disease, infertility, epilepsy, cancer & AIDS, Parkinsonism, anxiety, sleep dysfunction and Ischemia and hypoxia^[7,8].
Effect on the central nerve system
Uridine plays a crucial role in the pyrimidine metabolism of the brain. It supplies nervous tissue with the pyrimidine ring, and in turn, participates in a number of important metabolic pathways. Uridine and its nucleotide derivatives may also have an additional role in the function of the central nervous system as signaling molecules. Uridine administration had sleep-promoting and anti-epileptic actions, improved memory function and affected neuronal plasticity. Uridine can exert various kinds of effects on the central nerve system[CNS]^{[1, 8-10]} It was found to be an active component of sleep-promoting substances in our brain^{[11, 12, 2]} Anti-epileptogenic and anti-convulsant effect^{[3, 9, 10]} Thermoregulatory effect^{[4, 13]} long-term exposure to uridine improve our memory^{[5, 14]} involved in the regulation of neuronal plasticity through for example that it enhances neurite outgrowth^[15]. Based on those above findings, it can be used for the treatment of various diseases such as developmental delay, seizures, ataxia, severe language deficit, age-related cognitive decline and even Alzheimer's disease and Parkinson's disease. Uridine might also be useful as a nutrition supplement during development. Uridine[as uridine monophosphate] is found in mother's milk and has been proposed to play a role in regulatory mechanism through which plasma composition influences brain development^[16].
Cystic fibrosis
Cystic fibrosis is characterized by abnormal fluid transport across many epithelia including airways, pancreas, sweat glands and small intestine. This disease is associated with decreased Cl2 transport
and increased Na+ transport. The disease is caused by an absence or dysfunction of the cystic fibrosis transmembrane conductance regulator[CFTR], a Clchannel expressed by epithelial cells, and by an increase in active Na+ absorption[17, 18]. The uridine nucleotide can be used for the treatment of cystic fibrosis since UTP activates P2 purinoceptors, bypasses the defective Clsecretion to activate an alternative Ca2+ -dependent Clsecretory pathway, further stimulating Clsecretion in epithelial cells and decreased Na+ absorption^[18].
Effects on the circulatory system
The effects of uridine and its nucleotides on isolated blood vessels are complex, sometimes acting directly on smooth muscle cells, at other times stimulating surrounding endothelial cells. Uridine and its nucleotides produce opposing effects in some tissues, which suggests that these ligands could act at distinct receptors or via intracellular messenger systems. Further studies are warranted, because many of these effects were observed at potentially physiological levels, and could aid the development of a novel series of antihypertensive agents based on uridine analogues^[19].
Modulation of reproduction
An important function of uridine could be to promote sperm motility, as seminal plasma uridine concentrations are positively correlated to percentage sperm motility^[20]. It is perhaps relevant, therefore, that regulation of uridine diphosphatase during spermatogenesis in the rat was reported to be under hormonal control. The predominance of uridine in seminal fluids must lead to questions about its role in the environment of fertilization and implantation, but as yet these remain unanswered^[21].
Cancer and antiviral therapy
Uridine and UDP‹glucose have been used to counter the unwanted toxicity of pyrimidine-based anticancer drugs. Uridine has been used as a rescue therapy for myelotoxicity and gastrointestinal toxicity produced by 5-fluorouracil^[22]. Uridine and benzylacyclouridine protected mice against the neurotoxic side effects of pyrimidine-based drugs, such as azidothymidine used to treat HIV infection^[23].

Reference

www.cell.com/trends/pharmacological-sciences/pdf/S0165-6147(99]01298-5.pdf
https://www.trc-canada.com/product-detail/?CatNum=U829919&CAS=&Chemical_Name=Uridine[1’-D]&Mol_Formula=C?DH??N?O?
www.technologynetworks.com/genomics/lists/what-are-the-key-differences-between-dna-and-rna-296719
Berg JM, Tymoczko JL, Stryer L.[2002]. "Section 25.1In de Novo Synthesis, the Pyrimidine Ring Is Assembled from Bicarbonate, Aspartate, and Glutamine". Biochemistry[5th ed.]. W H Freeman.
Goetz, U, P. M. Da, and A. Pletscher. "Adenine-, guanineand uridine-5'-phosphonucleotides in blood platelets and storage organelles of various species. " Journal of Pharmacology & Experimental Therapeutics178.1(1971]:210-215.
L Ipata, P.; Pesi, R. Metabolic Regulation of Uridine in the Brain. Curr Metabolomics 2015, 3[1], 4-9.
Connolly, G. P., and J. A. Duley. "Uridine and its nucleotides: biological actions, therapeutic potentials. " Trends in Pharmacological Sciences20.5(1999]:218-25.
Dobolyi, and Arpad. Uridine Function in the Central Nervous System. Law, politics and the judicial system in Canada /. University of Calgary Press, 2011:743-751.
Yegutkin, G. G. Nucleotideand nucleoside-converting coenzymes: Important modulators of purinergic signalling cascade. Biochim. Biophys. Acta-Mol. Cell. Res., 2008, 1783, 673-694.
Burnstock, G. Physiology and pathophysiology of purinergic neurotransmission. Physiol. Rev., 2007, 87, 659-797.
Borbely, A. A.; Tobler, I. Endogenous sleep-promoting substances and sleep regulation. Physiol. Rev., 1989, 69, 605-670.
Inoue, S. Sleep and sleep substances. Brain Dev., 1986, 8, 469-473.
Peters, G. J.; van Groeningen, C. J.; Laurensse, E. J.; Lankelma, J.; Leyva, A.; Pinedo, H. M. Uridine-induced hypothermia in mice and rats in relation to plasma and tissue levels of uridine and its metabolites. Cancer Chemother. Pharmacol., 1987, 20, 101-108.
Teather, L. A.; Wurtman, R. J. Chronic administration of UMP ameliorates the impairment of hippocampal-dependent memory in impoverished rats. J. Nutr., 2006, 136, 2834-2837.
Pooler, A. M.; Guez, D. H.; Benedictus, R.; Wurtman, R. J. Uridine enhances neurite outgrowth in nerve growth factor-differentiated PC12 [corrected]. Neuroscience, 2005, 134, 207-214.
Wurtman, R. J. Synapse formation and cognitive brain development: effect of docosahexaenoic acid and other dietary constituents. Metabol. Clin. Exp., 2008, 57, S6-S10.
Knowles, Michael R, L. L. Clarke, and R. C. Boucher. "Activation by Extracellular Nucleotides of Chloride Secretion in the Airway Epithelia of Patients with Cystic Fibrosis." N Engl J Med 325.8(1991]:533-538.
Bennett, W D, et al. "Effect of uridine 5'-triphosphate plus amiloride on mucociliary clearance in adult cystic fibrosis. " American Journal of Respiratory & Critical Care Medicine 153.6 Pt 1(1996]:1796.
Seifert, R, and G. Schultz. "Involvement of pyrimidinoceptors in the regulation of cell functions by uridine and by uracil nucleotides. " Trends in Pharmacological Sciences 10.9(1989]:365-369.
Ronquist, G., B. Stegmayr, and F. Niklasson. "Sperm Motility and Interactions Among Seminal Uridine, Xanthine, Urate, and Atpase in Fertile and Infertile Men." Archives of Andrology 15.1(1985]:21-27.
Xuma, M, and R. W. Turkington. "Hormonal regulation of uridine diphosphatase during spermatogenesis in the rat." Endocrinology91.2(1972]:415.
Leyva, A, et al. "Phase I and pharmacokinetic studies of high-dose uridine intended for rescue from 5-fluorouracil toxicity. " Cancer Research 44.12 Pt 1(1984]:5928-5933.
Calabresi, P, et al. "Benzylacyclouridine reverses azidothymidine-induced marrow suppression without impairment of anti-human immunodeficiency virus activity." Blood 76.11(1990]:2210-5.

Chemical Properties

White powder; odorless; slightly acrid and faintly sweet taste. Soluble in water; slightly soluble in dilute alcohol; insoluble in strong alcohol.

Uses

Uridine is a nucleoside, contains a uracil attached to a ribose ring via a β-N1-glycosidic bond

Uses

Uridine is a nucleoside; widely distributed in nature. Uridine is one of the four basic components of ribonucleic acid (RNA)

Definition

ChEBI: Uridine is a ribonucleoside composed of a molecule of uracil attached to a ribofuranose moiety via a beta-N(1)-glycosidic bond. It has a role as a human metabolite, a fundamental metabolite and a drug metabolite. It is functionally related to a uracil.

General Description

Uridine is a pyrimidine nucleoside which is crucial for the synthesis of RNA and membranes. It helps in normal cell function and growth by forming pyrimidine nucleotide -lipid conjugates. Uridine has been studied as a rescue agent to reduce the toxicities associated with 5-fluorouracil (5-FU), thereby allowing the administration of higher doses of 5-FU in chemotherapy regimens. (NCI04)

Biochem/physiol Actions

Uridine monophosphate is essential for protein glycosylation, polysaccharide biosynthesis and lipid metabolism. Oral administration of uridine is suggested for anisopoikilocytosis and epileptic encephalopathy disorders. Uridine has numerous biological functions like treating dry eye syndrome, regulating nervous system and favors reproduction. High levels of uridine are implicated in insulin resistance.

target

ATPase | Potassium Channel | p53

Purification Methods

Crystallise -uridine from aqueous 75% MeOH or EtOH (m 165-166o). [Beilstein 24 III/IV 1202.]

Uridine Preparation Products And Raw materials

Raw materials

Preparation Products

Vidarabine Calcium 5'-ribonucleotide

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58-96-8, UridineRelated Search:

Cytidine NUCLEOSIDE PHOSPHORYLASE BACTERIAL 6-Methyluracil Doxifluridine Trifluorothymine 2',3',5'-TRI-O-BENZOYLURIDINE Ribavirin 5-Fluorocytidine 1-beta-D-Arabinofuranosyluracil Uracil UDP-ALPHA-D-GALACTOSE DISODIUM SALT Uridine 5'-diphosphoglucose disodium salt URIDINE 5'-MONOPHOSPHOMORPHOLIDATE 4-MORPHOLINE-N,N'-DICYCLOHEXYLCARBOXAMIDINE SALT cytidine 3',5'-diphosphate UTP Diquafosol Impurity 12 Diquafosol INS 365

TIMTEC-BB SBB000838

URACIL RIBOSIDE

URD

URACIL-1-BETA-D-RIBOFURANOSIDE

URACIL-3-RIBOSIDE

1-beta-d-ribofuranosyl-uraci

1-D-Ribofuranosyluracil

Uridine Uracil-1--D-ribofuranoside

URIDINE extrapure

1-β-D-Ribofuranosyluracil, Uracil-1-β-D-ribofuranoside

1-b-D-Ribofuranosyl-2,4(1H,3H)-pyrimidinedione

2,4(1H,3H)-pyrimidinedione-1

Arsenic acid (H3AsO4), lead salt

Lead(II) arsenate(III)

URIDINE(RG)

1-β-D-Ribofuranosyl-2,4(1H,3H)-pyrimidinedione

Uracil-3-D-ribofuranoside

1-((2R,3R,4S,5R)-3,4-dihydroxy-5-(hydroxyMethyl)tetrahydrofuran-2-yl)pyriMidine-2,4(1H,3H)-dione

1-beta-D-Ribofuranosyluracil, Uracil-1-beta-D-ribofuranoside

Uridine (15 mg)

NFDB2

1-[(2S,3S,4R,5S)-3,4-dihydroxy-5-(hydroxyMethyl)oxolan-2-yl]-1,2,3,4-tetrahydropyriMidine-2,4-dione

Uridine, 99% 100GR

Uridine, 99% 1GR

Uridine, 99% 25GR

NSC 20256

1-[(2R,3R,4S,5R)-3,4-dihydroxy-5-(hydroxyMethyl)oxolan-2-yl]-1,2,3,4-tetrahydropyriMidine-2,4-dione

1-(beta-D-Ribofuranosyl)uracil, Uracil riboside

Cytarabine Impurity 16(Cytarabine EP Impurity B)

Adenosine Impurity 6（Adenosine EP Impurity F）（Uridine）

1-[3,4-dihydroxy-5-(hydroxymethyl)tetrahydro-2-furanyl]-2,4(1H,3H)-pyrimidinedione (non-preferred name)

Powder Uridine CAS: 58-96-8

beta-D-Ribofuranoside, 2,4(1H,3H)-pyrimidinedione-1

beta-Uridine

d-Ribosyl uracil

Uracil, 1-beta-D-ribofuranosyl-

Uridin

D-URIDINE

1-BETA-D-RIBOFURANOSYLURACIL

1-BETA-RIBOFURANOSYL URACIL

3-B-D-RIBOFURANOSYLURACIL

URIDINE CELL CULTURE TESTED

URIDINE SIGMAULTRA

URIDINE, CRYSTALLIZED

URIDINE 99+%

URIDINE ULTRA PURE GRADE 99+%

uridine(UR)

Uracil-1-β-D-ribofuran

URIDINE,REAGENT

1-b-D-ribofuranosyl-uracil

Uracil-1-β-D-ribofuranoside

1-beta-D-Ribofuranosyl uracil (UR)

Uridine

Uridine Vetec(TM) reagent grade, 99%

ara-L-uridine

Diquafosol Impurity 11(URD)

1-beta-D-Ribofuranosylpyrimidine-2,