BETA-AMANITIN Property

Melting point:

300°

Boiling point:

1599.6±65.0 °C(Predicted)

Density 

1.1725 (rough estimate)

refractive index 

1.7400 (estimate)

storage temp. 

−20°C

solubility 

Water: 1 mg/ml

pka

4.02±0.10(Predicted)

form 

White to off-white powder.

color 

White to off-white

Safety

Hazard Codes 

T+

Risk Statements 

26/27/28

Safety Statements 

7-28-36/37/39-45

RIDADR 

UN 3462 6.1/PG 1

WGK Germany 

3

RTECS 

NJ8324000

HazardClass 

6.1(a)

PackingGroup 

I

HS Code 

2934999090

Toxicity

LD50 i.p. in albino mice: 0.4 mg/kg (Wieland, Wieland)

Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Danger

Precautionary statements

P260Do not breathe dust/fume/gas/mist/vapours/spray.

P262Do not get in eyes, on skin, or on clothing.

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P280Wear protective gloves/protective clothing/eye protection/face protection.

BETA-AMANITIN Chemical Properties,Usage,Production

Acute toxicity

peritoneal-mouse LD50: 0.4 mg/kg; unnamed-mouse LD50: 0.4 mg/kg

Mechanism of action

β-Amanitin is a cyclic peptide found in several species of the Amanita genus, as well as other poisonous fungi. It inhibits RNA polymerase II and III but not RNA polymerase I or bacterial RNA polymerase, thus preventing mammalian protein synthesis. The biological activity of this toxin is similar to that of α-amanitin but differs structurally by the presence of a carboxyl group, which is useful for coupling reactions.

Uses

β-Amanitin from Amanita phalloides has been used:

• as a calibration standard for the quantification of β-Amanitin using liquid chromatography-high resolution-mass spectrometry/mass spectrometry (LC-HR-MS/MS) method.
• to determine its concentration in urine samples by capillary zone electrophoresis (CZE).
• in the analysis of β-amanitin in toxic mushrooms by liquid chromatography coupled to time-of-flight mass spectrometry.

Uses

As a tool in molecular biology.

General Description

β-Amanitin belongs to the family of amatoxins. Amanitins are made of bicyclic octapeptides. β-Amanitin is composed of a carboxyl group and is acidic in nature.

Biochem/physiol Actions

Toxic constituent of the mushroom, Amanita phalloides, inhibits eukaryotic RNA polymerase II and III, but not RNA polymerase I or bacterial RNA polymerase. Inhibits mammalian protein synthesis.