Guanidine thiocyanate Property

Melting point:

120-122 °C(lit.)

Density 

1.103 g/mL at 20 °C

vapor pressure 

0Pa at 20℃

refractive index 

n20/D 1.482

storage temp. 

20-25°C

solubility 

H₂O: 6 M at 20 °C, clear, colorless

form 

Crystals or Crystalline Powder

pka

12.5[at 20 ℃]

Specific Gravity

1.152

color 

White or colorless

PH

4.8-6.0 (1420g/l, H2O, 20℃)(saturated solution)

Odor

Odorless

PH Range

4.8 - 6.0

Water Solubility 

Soluble in water and ethanol

Sensitive 

Light Sensitive

BRN 

3563461

Stability:

Stability Stable, but light sensitive. Incompatible with acids (contact releases very toxic gas), strong oxidising agents.

InChIKey

ZJYYHGLJYGJLLN-UHFFFAOYSA-N

LogP

-1.5--1.42 at 20℃

CAS DataBase Reference

593-84-0(CAS DataBase Reference)

EPA Substance Registry System

Thiocyanic acid, compd. with guanidine (1:1) (593-84-0)

Safety

Hazard Codes 

Xn,T,C

Risk Statements 

20/21/22-32-52/53-36/37/38-34

Safety Statements 

36/37-61-13-36-26-45-36/37/39

RIDADR 

1759

WGK Germany 

2

RTECS 

XL1225000

F 

3-10

TSCA 

Yes

HazardClass 

8

PackingGroup 

III

HS Code 

29252000

Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Danger

Hazard statements

H314Causes severe skin burns and eye damage

H412Harmful to aquatic life with long lasting effects

Precautionary statements

P260Do not breathe dust/fume/gas/mist/vapours/spray.

P273Avoid release to the environment.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P303+P361+P353IF ON SKIN (or hair): Remove/Take off Immediately all contaminated clothing. Rinse SKIN with water/shower.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

Guanidine thiocyanate Chemical Properties,Usage,Production

Description

Guanidine thiocyanate is one of the cheapest and easiest to prepare of the guanidine salts. Guanidine Thiocyanate is an ultrapure, molecular biology grade reagent. It is free of detectable nuclease and protease activity and is a strong protein denaturant, as both the guanidinium cation and the thiocyanate anion are chaotropic agents. It is provided in one bottle containing 500 g. It is recommended for isolation of RNA, especially for tissues such as pancreas with high levels of RNase activity. In solution, it is known as guanidinium thiocyanate. Guanidine Thiocyanate is thoroughly tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity.

Uses

Guanidine thiocyanate is a potent protein denaturant (stronger than guanidine HCl) often used in the isolation of intact ribonucleic acid to eliminate RNase activity. RNase can recover activity after boiling, but is irreversibly inactivated in a 4 M solution of guanidine thiocyanate. Such solutions, to which the reducing agent b-mercaptoethanol is often added, are used to inactivate RNAse when isolating RNA from tissues that are rich in RNase, such as liver. Total nuclear and cytoplasmic RNA may be isolated this way. A protocol for extracting RNA with guanidine isothiocyanate has been published.
In the presence of guanidine thiocyanate, proteins dissolve readily, cellular structures disintegrate and nucleoproteins dissociate from nucleic acids, as protein secondary structure is lost.

Biochem/physiol Actions

Guanidine thiocyanate is a denaturing agent and is used routinely in RNA isolation. It is used as a storage buffer for whole blood samples. Guanidine thiocyanate inactivates nucleases and is ideal for storing and freezing fecal samples for DNA studies. It is used in combination with phenol−chloroform in RNA extraction.

Toxicity evaluation

Guanidine thiocyanate is used commercially as a disinfectant and a general protein denaturant but its more common use is in the extraction of DNA and RNA in molecular biology. Нere are no reports of guanidine thiocyanate toxicity from oral ingestion. Toxicity of diوٴerent thiocyanate compounds other than guanidine thiocyanate has been described. Potassium thiocyanate was used over 50 years ago for the treatment of hypertension and its toxicity appears to cause a neurological disorder consisting of generalized weakness, delirium and a decrease level of consciousness. Reports of thiocyanate toxicity from nitroprusside use for hypertension in the setting of renal impairment/failure have also been reported as thiocyanate is renally cleared. In a fatal deliberate ingestion of a herbicide which contained ammonium thiocyanate and aminotriazole, a 54 year old man presented with coma and cardiovascular collapse although the relative contributions of the two herbicides to the clinical toxicity is unknown.

Chemical Properties

White cryst.powder

Uses

Potent protein denaturant used in isolation of intact DNA, RNA.

Uses

Guanidine thiocyanate is a useful tool for RNA isolation and protein solubilization. It is involved in the quantification of mRNA from hepatocytes. It acts as a medium for blood smaple lystae in order to maintain the integrity of nucleic acid. It is a chaotropic agent, mainly involved in the extraction of DNA and RNA. It is utilized to deactivate the influenza virus.

Uses

Guanidine thiocyanate has been used as a component of the stabilization buffer for RNA lysis. It can be used for deactivation of endogenous RNases leading to denaturation of nucleoprotein complexes. It is also suitable for denaturation of macromolecules such as nucleic acids and proteins and for RNA isolation.

General Description

Guanidine thiocyanate is chaotropic salts which lyse cells and along with nuclease binding matrix it is also seen to inhibit nuclease activity during extraction of DNA. It works as a strong RNase inhibitor in extraction buffers.

Hazard

Irritant.

Flammability and Explosibility

Not classified

Biochem/physiol Actions

Guanidine thiocyanate (GTC) plays a role in protein denaturation. It is involved in separation of ribosomal ribonucleic acid (rRNA) from ribosomes and denatures ribonuclease (RNase). GTC has been studied in the unfolding and refolding of fluorescent proteins (FP). It can be used along with phenol and chloroform for extraction of RNA.