Note
ChemicalBook > CAS DataBase List > 2',7'-Dichlorofluorescein

2',7'-Dichlorofluorescein

Note
Product Name
2',7'-Dichlorofluorescein
CAS No.
76-54-0
Chemical Name
2',7'-Dichlorofluorescein
Synonyms
2,7-DICHLOROFLUORESCEIN;DICHLOROFLUORESCEIN;FLUORESCEIN 27;Fluorescein 548;3,6-DICHLOROFLUORAN;2,7-Dichorofluorescin;FLUORESCEIN DICHLORIDE;DICHLOROFLUORESCEIN TS;dcorange25(fluorescein;DICHLORO (R) FLUORESCEIN
CBNumber
CB0690319
Molecular Formula
C20H10Cl2O5
Formula Weight
401.2
MOL File
76-54-0.mol
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2',7'-Dichlorofluorescein Property

Melting point:
280 °C (dec.)(lit.)
Boiling point:
514.77°C (rough estimate)
Density 
0.79 g/mL at 20 °C
refractive index 
1.5300 (estimate)
Flash point:
12°C (53.6°F)
storage temp. 
room temp
solubility 
alcohol: passes test
form 
crystalline
pka
4.46(at 25℃)
color 
orange to red-brown, powder
PH Range
4.0 (colorless) - 6.6 (green)
Water Solubility 
Soluble in ethanol and methanol. Slightly dimethyl sulfoxide. Insoluble in water.
λmax
509nm
BRN 
58009
Stability:
Stable. Incompatible with strong oxidizing agents.
Major Application
Electroluminescent devices, sol-gel silica films, integrated circuits, photoresists, lithographic printing plates, recording material, LED devices, photoconductors, Solid-state dye laser, lithographic printing plates, inks, Fluorescent pH sensors, emissive probes, chemotherapeutic treatment of disease, phototherapeutic treatment of disease, photodynamic treatment of disease
InChIKey
NZVMJBGGAQSNLI-UHFFFAOYSA-N
CAS DataBase Reference
76-54-0(CAS DataBase Reference)
EPA Substance Registry System
Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 2',7'-dichloro-3',6'-dihydroxy- (76-54-0)
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Safety

Hazard Codes 
Xi,F
Risk Statements 
36/37/38-36-67-11
Safety Statements 
26-36-16
RIDADR 
UN 1219 3/PG 2
WGK Germany 
3
RTECS 
NT8050000
TSCA 
Yes
HS Code 
3204 19 00
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Warning
Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

Precautionary statements

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

P337+P313IF eye irritation persists: Get medical advice/attention.

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
35848
Product name
2′,7′-Dichlorofluorescein
Purity
BioReagent, suitable for fluorescence, ≥90% (T)
Packaging
5g
Price
$96.4
Updated
2024/03/01
Sigma-Aldrich
Product number
35848
Product name
2′,7′-Dichlorofluorescein
Purity
BioReagent, suitable for fluorescence, ≥90% (T)
Packaging
25g
Price
$491
Updated
2024/03/01
Sigma-Aldrich
Product number
1.09676
Product name
2′,7′-Dichlorofluorescein
Purity
indicator ACS,Reag. Ph Eur
Packaging
5G
Price
$355
Updated
2024/03/01
TCI Chemical
Product number
D0371
Product name
2',7'-Dichlorofluorescein [for Fluorescent indicator]
Purity
>95.0%(HPLC)
Packaging
1g
Price
$24
Updated
2024/03/01
TCI Chemical
Product number
D0371
Product name
2',7'-Dichlorofluorescein [for Fluorescent indicator]
Purity
>95.0%(HPLC)
Packaging
25g
Price
$149
Updated
2024/03/01
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2',7'-Dichlorofluorescein Chemical Properties,Usage,Production

Note

Hans et al. report that 2',7'-Dichlorofluorescein (DCF) finds legitimate experimental use in the generation of standard curves used to calibrate DCFH2-DA experimental results, post-chromatography estimation of carbohydrates and in indirect fluorimetric detection in open tubular capillary chromatography (OTC-HPLC). However, DCF is inappropriate for demonstrating ROS as it is oxidized to a non-fluorescent compound in the presence of hydroxyl radicles[1].

Chemical Properties

red to yellow solid

Uses

Widely used probe, e.g. for determination of carbohydrates by fluorescence densitometry after TLC and determination of H2O2 by chemiluminescence. Fluorescent additives used in indirect fluorimetric detection in OTC-HPLC.

Uses

2',7'-Dichlorofluorescein is used as an indicator and as a cellular antioxidant activity. It is also used for the determination of carbohydrates and hydrogen peroxide by fluorescence densitometry and chemiluminescence respectively. It is used as an indicator for argentometry by Fajans method. It is also involved in the cellular antioxidant activity assay. It is utilized as an additive in fluorescence and laser studies.
A new fluorescent sensor for Cd2+ and its application in living cells imaging. Tetrahedron Lett. 2015, 56 (11), 1322-1327.
Colorimetric and fluorogenic signaling of fluoride ions by thiophosphinated dichlorofluorescein. Dyes Pigm. 2015, 112, 170-175.

Uses

2',7'-Dichlorofluorescein is used as an indicator and as a cellular antioxidant activity. It is also used for the determination of carbohydrates and hydrogen peroxide by fluorescence densitometry and chemiluminescence respectively. It is used as an indicator for argentometry by Fajans method. It is also involved in the cellular antioxidant activity assay. It is utilized as an additive in fluorescence and laser studies.

Definition

2',7'-dichlorofluorescein is a member of 2-benzofurans. It has a role as a fluorochrome. It derives from a fluorescin.

Application

2',7'-dichlorofluorescein has been used as an oxidation-sensitive fluorescent probe to measure ROS (reactive oxygen species) formation in cells.

Biological Activity

2',7'-dichlorofluorescein is an oxidation-sensitive fluorescent probe. 2′7′-dichlorofluorescein diacetate is oxidized to 2′7′-dichlorofluorescein in the presence of ROS (reactive oxygen species).

Biochem/physiol Actions

2′,7′-Dichlorofluorescein is an oxidation-sensitive fluorescent probe. 2′7′-dichlorofluorescein diacetate is oxidized to 2′7′-dichlorofluorescein in the presence of ROS (reactive oxygen species).

References

[1] Chander Hans. “2’,7’-Dichlorofluorescein (DCF) or 2’,7’-dichlorodihydrofluorescein diacetate (DCFH2-DA) to measure reactive oxygen species in erythrocytes.” Biomedicine & Pharmacotherapy (2021): 111512.

2',7'-Dichlorofluorescein Preparation Products And Raw materials

Raw materials

Preparation Products

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2',7'-Dichlorofluorescein Suppliers

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View Lastest Price from 2',7'-Dichlorofluorescein manufacturers

Hebei Weibang Biotechnology Co., Ltd
Product
dichlorofluorescein 76-54-0
Price
US $0.00/KG
Min. Order
1KG
Purity
99%
Supply Ability
500000kg
Release date
2024-11-04
Henan Fengda Chemical Co., Ltd
Product
dichlorofluorescein 76-54-0
Price
US $5.00-2.00/kg
Min. Order
1kg
Purity
99%
Supply Ability
g-kg-tons, free sample is available
Release date
2024-04-16
Hebei Mojin Biotechnology Co., Ltd
Product
dichlorofluorescein 76-54-0
Price
US $0.00/KG
Min. Order
1KG
Purity
99%
Supply Ability
50000KG/month
Release date
2023-10-16

76-54-0, 2',7'-DichlorofluoresceinRelated Search:

2',7'-DICHLOROFLUORESCIN DIACETATE 4',5'-DICHLOROFLUORESCEIN 2',7'-Dichlorofluorescein 2',7'-DICHLOROFLUORESCEIN SODIUM SALT 2′,7′-Dichlorodihydrofluorescein Diacetate FLUORESCEIN CHLORIDE 2,4,5,7-tetrabromo-12,15-dichlorofluorescein, dipotassium salt Fluorescein 6-CARBOXY-2',4,4',5',7,7'-HEXACHLOROFLUORESCEIN, SUCCINIMIDYL ESTER 5(6)-carboxy-2',7'-dichlorofluorescein diac. N-succinim. E,5(6)-CARBOXY-2',7'-DICHLOROFLUORESCEIN DIACETATE N-SUCCINIMIDYL ESTER 6'-(O-(CARBOXYMETHYL))-2',7'-DICHLOROFLUORESCEIN 3'-(O-(N-ACETYL-BETA-D-GLUCOSAMINIDE)) 5(6)-CARBOXY-2',7'-DICHLOROFLUORESCEIN, FOR FLUORESCENCE 6-Carboxy-2',7'-dichlorofluorescein 3',6'-Diacetate SucciniMidyl Ester 5-(and-6)-carboxy-2`,7`-dichlorofluorescein diacetate dichlorofluorescein sodium salt 4',5'-BIS[BIS(2-PYRIDYLMETHYL)AMINOMETHYL]-2',7'-DICHLOROFLUORESCEIN 5-(AND-6)-CARBOXY-2'':7''-DICHLOROFLUORESCEIN DIACETATE *MIXED ISOMERS* 2,7-Dichlorofluorescein *Fluorescence Reference Standard*

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  • 2',7'-Dichlorofluorescein, Reagent
  • 2,7-Dichloro-3,6-dihydroxyspiro[9H-xanthene-9,1'(3'H)-isobenzofuran]-3'-one
  • DCDHF
  • DC orange 25 (2',7'-dichlorofluorescein)
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  • 2',7'-DICHLOROFLUORESCEIN, REAGENT (ACS)2',7'-DICHLOROFLUORESCEIN, REAGENT (ACS)2',7'-DICHLOROFLUORESCEIN, REAGENT (ACS)
  • 2’,t-dichlorofluorescein
  • 9’-[9H]xanthen]-3-one,2’,7’-dichloro-3’,6’-dihydroxy-Spiro[isobenzofuran-1(3H)
  • dcorange25(fluorescein
  • dcorange25(fluorescein548)
  • spiro[isobenzofuran-1(3h),9’-[9h]xanthen]-3-one,2’,7’-dichloro-3’,6’-dihydroxy
  • 2-(2,7-dichloro-6-hydroxy-3-oxo-3-H-xanthen-9-yl)benzoic acid
  • 2,7-Dichorofluorescin
  • 2',7'-Dichloro-3',6'-dihydroxy-3H-spiro[isobenzofuran-1,9'-xanthen]-3-one
  • Dichloro (R) fluorescein 2',7'-Dichloro-3',6'-dihydroxyspiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one
  • Fluorescein, 2′,7′-dichloro-
  • 2′,7′-dichloro Uorescein
  • D And C Borange No. 25
  • 2',7'-DichL
  • orofL
  • uorescein
  • 2',7'-Dichlorofluorescein, 98%, reagent grade
  • 2',7'-Dichlorofluorescein [for Fluorescent indicator]&gt