Description References
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Dehydrocholic acid

Description References
Product Name
Dehydrocholic acid
CAS No.
81-23-2
Chemical Name
Dehydrocholic acid
Synonyms
DHC;dehydrocholic;Acolen;Atrocholin;Dehydrocholate;3,7,12-trioxo-5β-cholanic acid;5β-CHOLANIC acid-3, 7, 12-TRIONE;Dee-Co;Biochol;Dehycol
CBNumber
CB0704485
Molecular Formula
C24H34O5
Formula Weight
402.53
MOL File
81-23-2.mol
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Dehydrocholic acid Property

Melting point:
238-240 °C
alpha 
29 º (c=2, dioxane)
Boiling point:
444.65°C (rough estimate)
Density 
1.0687 (rough estimate)
vapor pressure 
0.013Pa at 20℃
refractive index 
30.5 ° (C=2, Dioxane)
storage temp. 
Sealed in dry,Room Temperature
solubility 
ethanol: 10 mg/mL
pka
pKa 5.12(H2O t = 20 c > CMC) (Uncertain)
form 
Solid
color 
White to Off-White
Water Solubility 
65mg/L(30 ºC)
Merck 
14,2868
BRN 
3226734
LogP
1.64 at 22℃ and pH5.7
Surface tension
56.9mN/m at 63mg/L and 20℃
CAS DataBase Reference
81-23-2(CAS DataBase Reference)
NIST Chemistry Reference
Dehydrocholic acid(81-23-2)
EPA Substance Registry System
Cholan-24-oic acid, 3,7,12-trioxo-, (5.beta.)- (81-23-2)
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Safety

Safety Statements 
24/25
WGK Germany 
2
RTECS 
FZ2300000
TSCA 
Yes
HS Code 
2918300000
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Warning
Hazard statements

H303May be harmfulif swallowed

Precautionary statements

P270Do not eat, drink or smoke when using this product.

P301+P312IF SWALLOWED: call a POISON CENTER or doctor/physician IF you feel unwell.

P403Store in a well-ventilated place.

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
1166502
Product name
Dehydrocholic acid
Purity
United States Pharmacopeia (USP) Reference Standard
Packaging
200mg
Price
$164.8
Updated
2024/03/01
Cayman Chemical
Product number
29176
Product name
Dehydrocholic Acid
Packaging
1g
Price
$32
Updated
2024/03/01
Cayman Chemical
Product number
29176
Product name
Dehydrocholic Acid
Packaging
5g
Price
$93
Updated
2024/03/01
TRC
Product number
D229340
Product name
Dehydrocholic acid
Packaging
50g
Price
$100
Updated
2021/12/16
Biosynth Carbosynth
Product number
FD11044
Product name
Dehydrocholic acid
Packaging
50g
Price
$135.5
Updated
2021/12/16
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Dehydrocholic acid Chemical Properties,Usage,Production

Description

Dehydrocholic acid is a semisynthetic bile acid, which is made by the oxidation of cholic acid by chromic acid. It can increase the output of bile by the liver and the filling of the gallbladder. Dehydrocholic acid aids the digestion of fats and increases absorption of fat soluble vitamins.
It is used as a gastrointestinal agent that stimulates the flow of bile into the duodenum (cholagogue) or stimulate the production of bile by the liver (choleretic). It is also used as laxative to relief constipation, diuretic, and a diagnostic aid.

References

[1] Giancarlo Cravotto, Arianna Binello, Luisa Boffa, Ornelio Rosati, Marco Boccalini, Stefano Chimichi (2006) Regio- and stereoselective reductions of dehydrocholic acid, Steroids, 469-475
[2] https://pubchem.ncbi.nlm.nih.gov/compound/dehydrocholic_acid

Chemical Properties

white to off-white amorphous powder

Originator

Dehydrocholic Acid,New Zealand Pharmaceuticals Limited (NZP)

Uses

Dehydrocholic Acid is a derivative of Cholic Acid (C432600). a choleretic produced by, and isolated from liver cells.

Uses

antibacterial

Definition

ChEBI: 3,7,12-trioxo-5beta-cholanic acid is an oxo-5beta-cholanic acid in which three oxo substituents are located at positions 3, 7 and 12 on the cholanic acid skeleton. It has a role as a gastrointestinal drug. It is an oxo-5beta-cholanic acid, a 7-oxo steroid, a 12-oxo steroid and a 3-oxo-5beta-steroid. It is a conjugate acid of a 3,7,12-trioxo-5beta-cholan-24-oate.

Manufacturing Process

A.) Oxidation of cholic acid:
A solution, consisting of 15.40 g of cholic acid and 18.75 g of anhydrous sodium acetate in a solvent mixture of 20 ml of ethyl acetate, 30 ml of glacial acetic acid, and 30 ml of water, was prepared. This solution was cooled to 20°C. Chlorine gas was bubbled into the solution with vigorous stirring while the reaction temperature was maintained at 20°C. The chlorine was delivered at a constant rate of about 2.5 g per hour over a 4-hour period. The total amount of chlorine gas was 9.80 g which corresponds to about 3.68 moles per mole of cholic acid, or approximately a 23% excess. The solution temperature was maintained in the range of 16° to 20°C during the entire addition of chlorine. Initially the cholic acid solution was very dark-colored. As the reaction progressed, the solution became pale yellow and a precipitate of sodium chloride deposited. A considerable amount of product and sodium chloride precipitated during the latter stages of the reaction so that the final reaction mixture was a heavy slurry which was difficult to stir. After the addition of chlorine was complete, the slurry was aged one hour with stirring at 20°C. The excess chlorine was then discharged by dropwise addition of 10% aqueous sodium sulfite until the solution gave a negative test to starchiodide paper. The semi-crystalline slurry was then diluted with water to raise the total volume to 225 ml. The water was added dropwise with stirring over a 1-hour period. The ethyl acetate was then distilled off at 65-88°C. The resulting crystalline slurry was cooled to below 70°C and filtered through a sintered-glass funnel of medium porosity. The filter cake was washed until the filtrate gave a negative halide test with silver nitrate solution and then was sucked partially dry on the funnel. Drying was completed in a drier at 110°C for 3 hours. The product was crude pale tan dehydrocholic acid. Yield 14.3 (95%); M.P. 225-231°C.
B.) Purification of dehydrocholic acid:
To a chromatographic column, packed with 6.67 g of charcoal ("Nuchar C") with layers of sea sand at either end, 75 ml of acetone was added to wet the carbon. The column was heated to 40°C, and 25 ml of acetone was drained off. A solution of 20 g of dry crude dehydrocholic acid in 500 ml of acetone was poured into a reservoir atop the column and maintained in this reservoir at 40°C. This solution was then allowed to drop through the column at a constant rate over a 3-hour period. The column was then washed with 250 ml of acetone flowing through the column at a constant rate over a 1-hour-period at 40°C. The column effluent and wash acetone were combined and concentrated to a residual volume of about 100 ml which resulted in the formation of a thick slurry. The slurry was cooled with stirring at 0° to 5°C and aged for 30 min at this temperature. The slurry was filtered and the filter cake washed with cold acetone. The filter cake of U.S.P. dehydrocholic acid was sucked partially dry on the filter and then dried at 110°C for 3 hours. Yield 15 g to 17 g (75% to 85%).
A second crop of crystals was obtained from the combined filtrate and wash liquid from the first crop filtration. This mixture, which initially had a volume of about 100 ml, was concentrated to 20 ml. 10 ml of water was added to the solution and 10 ml of acetone mixed with a small amount of water distilled off. The residual thick slurry of dehydrocholic acid was cooled to 0-5°C, aged at this temperature with stirring for 30 min, and filtered. The filter cake was washed with acetone at 0°C, partially dried by suction on the filter, and then dried for three hours at 110°C. Yield 1 to 2 g (5% to 10%).

brand name

Decholin Sodium (Bayer).

Therapeutic Function

Choleretic, Diuretic, Diagnostic aid

Biochem/physiol Actions

Dehydrocholic acid is an oxidation product of cholic acid by chromic acid that is not present in physiological conditions. When used in animal experiments, it stimulates bile secretion.

Dehydrocholic acid Preparation Products And Raw materials

Raw materials

Preparation Products

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Dehydrocholic acid Suppliers

Shanghai xinchenfa Chemical Technology Co.,Ltd
Tel
021-13564487020 13564487020
Email
3546809660@qq.com
Country
China
ProdList
511
Advantage
58
Hubei Hongfuda Biotechnology Co., Ltd.
Tel
191-07255884 19107255884
Email
259506217@qq.com
Country
China
ProdList
1706
Advantage
58
J & K SCIENTIFIC LTD.
Tel
010-82848833 400-666-7788
Fax
86-10-82849933
Email
jkinfo@jkchemical.com
Country
China
ProdList
96815
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76
Meryer (Shanghai) Chemical Technology Co., Ltd.
Tel
021-61259108 18621169109
Fax
86-21-61259102
Email
market03@meryer.com
Country
China
ProdList
40228
Advantage
62
3B Pharmachem (Wuhan) International Co.,Ltd.
Tel
821-50328103-801 18930552037
Fax
86-21-50328109
Email
3bsc@sina.com
Country
China
ProdList
15839
Advantage
69
Alfa Aesar
Tel
400-6106006
Fax
021-67582001/03/05
Email
saleschina@alfa-asia.com
Country
China
ProdList
30123
Advantage
84
TCI (Shanghai) Development Co., Ltd.
Tel
021-67121386
Fax
021-67121385
Email
Sales-CN@TCIchemicals.com
Country
China
ProdList
24529
Advantage
81
Energy Chemical
Tel
021-021-58432009 400-005-6266
Fax
021-58436166
Email
sales8178@energy-chemical.com
Country
China
ProdList
44688
Advantage
61
LGM Pharma
Tel
1-(800)-881-8210
Fax
615-250-9817
Email
inquiries@lgmpharma.com
Country
United States
ProdList
2123
Advantage
70
Shanghai Hanhong Scientific Co.,Ltd.
Tel
021-54306202 13764082696
Email
info@hanhongsci.com
Country
China
ProdList
42958
Advantage
64
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View Lastest Price from Dehydrocholic acid manufacturers

Sinoway Industrial co., ltd.
Product
Dehydrocholic Acid 81-23-2
Price
US $0.00-0.00/kg
Min. Order
1kg
Purity
99%
Supply Ability
20tons
Release date
2024-05-20
WUHAN FORTUNA CHEMICAL CO., LTD
Product
Dehydrocholic acid 81-23-2
Price
US $0.00/KG
Min. Order
1KG
Purity
98%min
Supply Ability
500KGS
Release date
2021-06-15
Shaanxi Dideu Medichem Co. Ltd
Product
Dehydrocholic acid 81-23-2
Price
US $1.00-1.00/Kg/Bag
Min. Order
1g
Purity
99%
Supply Ability
50tons
Release date
2020-04-30

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