ChemicalBook > CAS DataBase List > 2-Nitrophenyl-beta-D-galactopyranoside

2-Nitrophenyl-beta-D-galactopyranoside

Product Name
2-Nitrophenyl-beta-D-galactopyranoside
CAS No.
369-07-3
Chemical Name
2-Nitrophenyl-beta-D-galactopyranoside
Synonyms
ONPG;o-NPG=ONPG;2-Nitrophenyl;ONPG Solution;ONP-BETA-D-GAL;2-Nitrophenyl-beta-D;2-Nitrophenyl-b-D-galactopy;2-Nitrophenyl hexopyranoside;ONP BETA-D-GALACTOPYRANOSIDE;2-Nitrophenyl-β-D-galactoside
CBNumber
CB1366392
Molecular Formula
C12H15NO8
Formula Weight
301.25
MOL File
369-07-3.mol
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2-Nitrophenyl-beta-D-galactopyranoside Property

Melting point:
186-193 °C (dec.)
alpha 
-70 º (c=1, H2O)
Boiling point:
442.39°C (rough estimate)
Density 
1.3709 (rough estimate)
refractive index 
-68.8 ° (C=1, H2O)
storage temp. 
2-8°C
solubility 
DMSO (Slightly), Water (Slightly, Heated, Sonicated)
pka
12.69±0.70(Predicted)
form 
Powder
color 
White to very slightly yellow
optical activity
[α]20/D 70±1°, c = 1% in H2O
Water Solubility 
Soluble in water.
BRN 
92207
InChIKey
KUWPCJHYPSUOFW-RMPHRYRLSA-N
CAS DataBase Reference
369-07-3(CAS DataBase Reference)
NIST Chemistry Reference
Galactopyranoside, o-nitrophenyl, beta-d-(369-07-3)
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Safety

Hazard Codes 
Xi
Risk Statements 
36/37/38
Safety Statements 
22-24/25-36-26
WGK Germany 
3
F 
3-10-21
HS Code 
29400090
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Warning
Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P271Use only outdoors or in a well-ventilated area.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P302+P352IF ON SKIN: wash with plenty of soap and water.

P304+P340IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

P312Call a POISON CENTER or doctor/physician if you feel unwell.

P403+P233Store in a well-ventilated place. Keep container tightly closed.

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
48712-M
Product name
o-Nitrophenyl-β-D-galactopyranoside - CAS 369-07-3 - Calbiochem
Purity
A β-galactosidase substrate for colorimetric and EIA applications; counterpart of widely used pNPP/alkaline phosphatase substrate.
Packaging
1g
Price
$54.7
Updated
2024/03/01
Sigma-Aldrich
Product number
48712-M
Product name
o-Nitrophenyl-β-D-galactopyranoside - CAS 369-07-3 - Calbiochem
Purity
A β-galactosidase substrate for colorimetric and EIA applications; counterpart of widely used pNPP/alkaline phosphatase substrate.
Packaging
5G
Price
$208
Updated
2024/03/01
Sigma-Aldrich
Product number
73660
Product name
2-Nitrophenyl β-D-galactopyranoside
Purity
≥99.0% (HPLC)
Packaging
1G
Price
$40.3
Updated
2022/05/15
Sigma-Aldrich
Product number
73660
Product name
2-Nitrophenyl β-D-galactopyranoside
Purity
≥99.0% (HPLC)
Packaging
5G
Price
$142
Updated
2022/05/15
TCI Chemical
Product number
N0418
Product name
2-Nitrophenyl beta-D-Galactopyranoside [Substrate for beta-D-Galactosidase]
Purity
>98.0%(HPLC)
Packaging
1g
Price
$30
Updated
2021/12/16
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2-Nitrophenyl-beta-D-galactopyranoside Chemical Properties,Usage,Production

Description

2-Nitrophenyl-β-D-galactopyranoside (ONPG) is a colorimetric and spectrophotometric substrate for detecting β-galactosidase activity. This compound is usually colorless. However, if β-galactosidase is present, it hydrolyzes the ONPG molecule into galactose and ortho-nitrophenol. The latter compound has a yellow color that can be used to check for enzyme activity utilizing a colorimetric assay (at 420 nm wavelength). β-Galactosidase is required for lactose utilization, so the intensity of the color produced can be used to measure the enzymatic rate. Though ONPG mimics lactose and is hydrolyzed by β-galactosidase, it cannot act as an inducer for the lac operon. Without another lactose analog that can act as an inducer, such as isopropyl β-D-1-thiogalactopyranoside (IPTG), β-galactosidase will not be transcribed and ONPG will not be hydrolyzed.

Chemical Properties

White Crystalline Solid

Uses

2-Nitrophenyl-beta-D-galactopyranoside is a β-Galactosidase substrate for colorimetric and EIA applications; counterpart of widely employed pNPP/alkaline phosphatase substrate.

Definition

ChEBI: A beta-D-galactoside having a 2-nitrophenyl substituent at the anomeric position.

General Description

ONPG (2-Nitrophenyl β-D-galactopyranoside) is a colorimetric substrate for β-galactosidase.

Biochem/physiol Actions

β-galactosidase, breaks down lactose into galactose and glucose. β-Galactosidase is not lactose specific and can act on simple galactosides. 2-Nitrophenyl β-D-galactopyranoside hydrolysis results in the release of galactose and a yellow chromogenic compound. The test substrate does not depend on an induced or constitutive permease enzyme to enter the cell, therefore reactions are rapid and occur within a 24-hour period.

Purification Methods

Purify 2-nitrophenyl--D-galactopyranoside by recrystallisation from EtOH. [Seidman & Link J Am Chem Soc 72 4324 1950, Snyder & Link J Am Chem Soc 75 1758 1953]. It is a chromogenic substrate for -galactosidases [Jagota et al. J Food Sci 46 161 1981]. [Beilstein 17/7 V 52.]

2-Nitrophenyl-beta-D-galactopyranoside Preparation Products And Raw materials

Raw materials

Preparation Products

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2-Nitrophenyl-beta-D-galactopyranoside Suppliers

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View Lastest Price from 2-Nitrophenyl-beta-D-galactopyranoside manufacturers

WUHAN FORTUNA CHEMICAL CO., LTD
Product
2-Nitrophenyl-beta-D-galactopyranoside 369-07-3
Price
US $0.00/KG
Min. Order
1KG
Purity
98.0%
Supply Ability
500kg/month
Release date
2023-01-09
Hebei Weibang Biotechnology Co., Ltd
Product
2-Nitrophenyl-beta-D-galactopyranoside 369-07-3
Price
US $0.00-0.00/KG
Min. Order
1KG
Purity
99%
Supply Ability
500000kg
Release date
2024-11-05
Hebei Yanxi Chemical Co., Ltd.
Product
2-Nitrophenyl-beta-D-galactopyranoside 369-07-3
Price
US $40.00/kg
Min. Order
1kg
Purity
0.99
Supply Ability
10 tons
Release date
2023-09-25

369-07-3, 2-Nitrophenyl-beta-D-galactopyranosideRelated Search:

2-NITROPHENYL-BETA-D-GLUCOPYRANOSIDE 2-NITROPHENYL-ALPHA-D-GALACTOPYRANOSIDE Sodium 3-nitrobenzenesulphonate 4-Nitrobenzoic acid 4-Nitrobenzaldehyde α-galactosidase DIGITONIN Nitrobenzene 4-Nitrophenyl-beta-D-galactopyranoside 1,3-Dinitrobenzene 4-NITROPHENYL-BETA-D-GALACTOPYRANOSIDE, 98+%,4-NITROPHENYL-BETA-D-GALACTOPYRANOSIDE HYDRATE,4-Nitrophenyl beta-D-galactopyranoside 2-hexadecanoylamino-4-nitrophenyl-beta-D-galactopyranoside 2-NITROPHENYL-BETA-D-GALACTOPYRANOSIDE-6-PHOSPHATE DICYCLOHEXYL P-NITROPHENYL-BETA-D-GALACTOPYRANOSIDE TETRAACETATE O-NITROPHENYL BETA-D-GALACTOPYRANOSIDE-6-PHOSPHATE, CYCLOHEXYLAMMONIUM SALT m-nitrophenyl beta-D-galactopyranoside ,3-NITROPHENYL-BETA-D-GALACTOPYRANOSIDE 2-CHLORO-4-NITROPHENYL-BETA-D-GALACTOPYRANOSIDE ρ-Nitrobenzenesulphonic acid Onpg Solution

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  • beta-d-galactopyranoside,2-nitrophenyl
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  • o-NPG=ONPG
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  • 2-nitrophenyl-á-d-galactopyranoside
  • ONPG（2-Nitrophenyl-beta-D-galactopyranoside
  • 2-Nitrophenyl b-D-galactoside-(1,5), ONPG
  • 2-Nitrophenyl-β-D-galactoside
  • 2-Nitrophenyl β-D-Galactopyranoside [Substrate for β-D-Galactosidase]
  • ortho-Nitrophenyl-β-galactoside
  • (2R,3R,4S,5R,6S)-2-(hydroxyMethyl)-6-(2-nitrophenoxy)tetrahydro-2H-pyran-3,4,5-triol
  • Ortho nitroalpha -D-glucopyranoside
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  • ortho-nitrophenyl β-D-galactopyranoside
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  • 2-nitrophenyl β-d-[2-13C]galacto-pyranoside
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  • (2R,3R,4S,5R,6S)-2-(Hydroxymethyl)-6-(2-nitrophenoxy)oxane-3,4,5-triol
  • O-Nitrophenyl-beta-d-galactopyranoside 99%
  • 2-Nitrophenyl-^b-D-galactopyranoside, 98+%
  • 2-Nitrophenyl-b-D-galactopy
  • 2-Nitrophenyl-b-D-glucosidase
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  • 2-Nitrophenyl β-D-galactopyranoside,o-Nitrophenyl β-D-galactopyranoside, ONPG
  • 2-Nitrophenyl-beta-D
  • 2-NITROPHENYL-SS-D-GALACTOPYRANOSIDE
  • 2-Nitrophenyl b-D-galactopyranoside - non-animal origin
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