DMG-PEG 2000 Property

storage temp. 

-20°C, sealed storage, away from moisture and light

solubility 

DMSO: 100 mg/mL

form 

A crystalline solid

color 

White to light yellow

Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Warning

Hazard statements

H302Harmful if swallowed

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P301+P312IF SWALLOWED: call a POISON CENTER or doctor/physician IF you feel unwell.

P302+P352IF ON SKIN: wash with plenty of soap and water.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

DMG-PEG 2000 Chemical Properties,Usage,Production

Description

DMG-PEG 2000, also called 1,2-dimyristoyl-rac-glycero-3-methoxypolyethylene glycol-2000, is used for liposome preparation. DMG-PEG 2000 has become known for lipid nanoparticle (LNP) preparation for siRNA (small interfering RNA) delivery in the COVID-19 vaccine development. DMG-PEG 2000 has been used in studies for anticancer drug delivery studies for hepatocellular, gastrointestinal, neuroendocrine and adrenocortical carcinoma.

Uses

DMG-PEG2000 is used for the preparation of liposomes for siRNA delivery, which can improve the transfection efficiency. It can also be used for the preparation of lipid nanoparticles for in vivo delivery of orally administered plasmid DNA. DMG-PEG2000 can improve the mucus permeability and delivery efficiency of nanoparticles.

Chemical Properties

DMG-PEG 2000 is soluble in ethanol, DMSO and chloroform:methanol (9:1) at a concentration of 5 mg/mL; store at -20°C. Generally transported on dry ice.

Application

DMG-PEG 2000 is a lipid excipient that has been used in combination with other lipids to form lipid nanoparticles (LNPs) for delivery of COVID-19 mRNA vaccines.

in vitro

NP-3 (0.05-1.6 mg/mL; 24 hours) does not decrease the cytotoxicity of cells in 293T, HepG2, A549, and HeLa cell lines, but the DPPC and DMG-PEG coated nanoparticles reduce cell cytotoxicity. In addition, the transfection efficiency of DPPC/DMG-PEG/(lPEI/DNA) nanoparticles (NP-3) in 293 cells is improved, and the maximum transfection efficiency (～76% eGFP positive cells) is observed.

in vivo

NP-3 (oral administration; 150 μg DNA per mouse; single dose) at 12, 24, and 36 h postadministration, luciferin substrate is intraperitoneally injected to verify its permeability. NP-3 group maintains high luciferase expression in the liver, lung, and intestine areas 12-24 h post-treatment.Additionally, NP-3 exhibits 1.5 times higher signal intensity than that of NP-1 or NP-2 group from 12 to 24 h postoral administration.