EIPA Property

Melting point:

202-205 °C(lit.)

Density 

1.49±0.1 g/cm3(Predicted)

storage temp. 

Keep in dark place,Sealed in dry,2-8°C

solubility 

Acetonitrile (Slightly, Heated), DMSO (Slightly), Methanol (Slightly)

pka

8.67±0.46(Predicted)

form 

Off-white to yellow solid.

color 

Pale Yellow to Green

Stability:

Unstable in methanol

CAS DataBase Reference

1154-25-2

Safety

Hazard Codes 

Xi

Risk Statements 

36/37/38

Safety Statements 

26-37/39

RIDADR 

2811

WGK Germany 

3

HazardClass 

6.1(b)

PackingGroup 

III

Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Warning

Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P271Use only outdoors or in a well-ventilated area.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P302+P352IF ON SKIN: wash with plenty of soap and water.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

EIPA Chemical Properties,Usage,Production

Description

Sodium-hydrogen exchangers (NHE) are involved in maintaining sodium and pH balance in a variety of tissues. They are also known as sodium-hydrogen antiporters and solute carrier family 9 members. 5-(N-ethyl-N-isopropyl)-Amiloride (EIPA) is a potent inhibitor of several NHE isoforms, inhibiting NHE1, NHE2, NHE3, and NHE5 with K_i values of 0.02, 0.5, 2.4, and 0.42 μM, respectively. It less effectively inhibits NHE4 (IC₅₀ ≥10 μM). EIPA is commonly used at a concentration of 5-10 μM to inhibit cellular HNE activity.

Chemical Properties

Yellow Solid

Uses

Selective Na+/H+ antiporter inhibitor. Selectively enhances the excretion of sodium ions without causing an increase in excretion of potassium ions.

Uses

5-(N-Ethyl-N-isopropyl)amiloride has been used:

• as a supplement in endothelial cell growth medium to incubate human lung microvascular endothelial cell
• in Pluronic F-127 stock solution for Drosophila larval live-imaging
• in choline chloride solution as an experimental perfusate, to study the effect of Na⁺/H⁺ inhibition on jejunal loop perfusion in vivo

Definition

ChEBI: Ethylisopropylamiloride is a member of the class of pyrazines that is amiloride in which the amino substitutent of the pyrazine ring that is adjacent to the chloro substituent has been substituted by an ethyl group and by an isopropyl group. It has a role as an anti-arrhythmia drug, a neuroprotective agent and a sodium channel blocker. It is a member of guanidines, an aromatic amine, an organochlorine compound, a tertiary amino compound, a member of pyrazines and a monocarboxylic acid amide. It is functionally related to an amiloride.

Biochem/physiol Actions

Selective blocker of Na+/H+ antiport

storage

Store at +4°C