Description References
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Cinobufagin

Description References
Product Name
Cinobufagin
CAS No.
470-37-1
Chemical Name
Cinobufagin
Synonyms
FEMA 2288;AKOS 233-01;CINOBUFAGIN;16-beta)-bet;cinobufagine;AKOS B004228;CINOBUFAGIN(P);Cinobufagin-RM;Cinobufagin std.;Cinobufagin (6CI)
CBNumber
CB2206701
Molecular Formula
C26H34O6
Formula Weight
442.55
MOL File
470-37-1.mol
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Cinobufagin Property

Melting point:
133 °C(lit.)
Boiling point:
300 °C(lit.)
Density 
1.261 g/cm3
Flash point:
>230 °F
storage temp. 
2-8°C
solubility 
DMF: 10 mg/ml; DMSO: 10 mg/ml; DMSO:PBS (pH 7.2) (1:2): 0.33 mg/ml
pka
15.10±0.70(Predicted)
form 
powder to crystal
color 
White to Light yellow
InChIKey
SCULJPGYOQQXTK-QZXYOKJUNA-N
SMILES
C[C@]12CC[C@]3([H])[C@]4(CC[C@H](O)C[C@@]4([H])CC[C@@]3([H])[C@@]31O[C@@H]3[C@H](OC(=O)C)[C@@H]2C1=COC(=O)C=C1)C |&1:1,4,6,9,12,16,18,20,21,26,r|
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Safety

Hazard Codes 
Xi,T+,Xn
Risk Statements 
36/37/38-26/27/28-42/43-20/22
Safety Statements 
26-36-45-36/37/39-22-24/25
RIDADR 
UN 2811 6.1/PG 1
WGK Germany 
3
RTECS 
GD7850000
HazardClass 
6.1(a)
PackingGroup 
II
HS Code 
29322090
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Danger
Hazard statements

H300Fatal if swallowed

H310Fatal in contact with skin

H330Fatal if inhaled

Precautionary statements

P260Do not breathe dust/fume/gas/mist/vapours/spray.

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P284Wear respiratory protection.

P302+P350IF ON SKIN: Gently wash with plenty of soap and water.

P310Immediately call a POISON CENTER or doctor/physician.

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
SML3135
Product name
Cinobufagin
Purity
≥98% (HPLC)
Packaging
5 mg
Price
$97.85
Updated
2025/07/31
Sigma-Aldrich
Product number
SML3135
Product name
Cinobufagin
Purity
≥98% (HPLC)
Packaging
25 mg
Price
$412
Updated
2025/07/31
TCI Chemical
Product number
C3460
Product name
Cinobufagine
Purity
>98.0%(HPLC)
Packaging
25mg
Price
$274
Updated
2025/07/31
TCI Chemical
Product number
C3460
Product name
Cinobufagine
Purity
>98.0%(HPLC)
Packaging
100mg
Price
$817
Updated
2025/07/31
Cayman Chemical
Product number
19844
Product name
Cinobufagin
Packaging
5mg
Price
$115
Updated
2024/03/01
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Cinobufagin Chemical Properties,Usage,Production

Description

Cinobufagin is a cardiotoxic bufanolide steroid secreted by the Asiatic toad Bufo gargarizans, having similar effects to digitalis and used in traditional Chinese medicine. It has potential antineoplastic activity and now is widely used in clinical practice, especially in anti-liver cancer. It has benn found to suppress cancer cell proliferation and cause apoptosis in cancer cells via a sequence of apoptotic modulators, including mitochondrial Bax and cytosolic chromosome c, and caspases 3, 8, and 9.

References

1. https://en.wikipedia.org/wiki/Cinobufagin
2. https://www.cancer.gov/publications/dictionaries/cancer-drug?cdrid=689412
3. http://www.yourdictionary.com/cinobufagin
4. https://www.medchemexpress.com/cinobufagin.html

Description

Cinobufagin is a cardiotonic steroid that has been found in the skin of toads of genus Bufo and has diverse biological activities. It inhibits Na+/K+-ATPase activity in guinea pig heart ventricular muscle homogenates by 45% when used at a concentration of 0.3 μM. Cinobufagin is cytotoxic to HCT116 colorectal cancer cells in vitro with IC50 values of less than 50 ng/ml at 48- and 72-hour time points and induces apoptosis in a concentration-dependent manner. In vivo, cinobufagin (10 mg/kg) reduces tumor growth in an HCT116 mouse xenograft model. Cinobufagin (1 μg/ml) inhibits LPS-induced expression of MHC class II, CD80, and CD86 and release of IL-6, IL-8, TNF-α, and IL-10 in human monocyte-derived dendritic cells. It also increases expression of the antimicrobial peptides hBD2 and hBD3 in dendritic cells. Cinobufagin exhibits dose-dependent antinociceptive effects in the hot-plate, acetic acid writhing, and formalin tests in mice.

Chemical Properties

Soluble in chloroform, methanol and other organic solvents, insoluble in water. Derived from the dried secretion of toads such as Bufo bufo gargarizans Cantor.

Uses

Cinobufagine is a major component of cinobufacini (huachansu), is an important cardenolidal steroid. Cinobufagine was shown to exhibit potent anti-cancer effects.

Definition

ChEBI: Cinobufagin is a steroid lactone. It is functionally related to a bufanolide.

in vivo

Cinobufagin (5 mg/kg for i.p., once a day for 10 days) inhibits xenograft growth by inducing cell apoptosis in tumor xenograft mice model[1].
Cinobufagin (5 mg/kg for i.p., once a day for 10 days) suppresses tumor growth in both subcutaneous and intracranial U87MG-EGFR xenograft mouse models and increases the median survival of nude mice bearing intracranial U87MGEGFR tumors[2].

Animal Model:OCM1 cells tumor xenograft in Nu/Nu nude mice [1]
Dosage:5 mg/kg
Administration:Intraperitoneal injection (i.p.), once a day for 10 days
Result:Made the tumors grew more slowly than those treated with intraperitoneal injection of saline or untreated.
Increased the expression of caspase-3 and PARP in tumor tissues and decreased Bcl-2 and Bcl-xl expression in mouse tumor tissues and increased expression of Bad and Bax.
Animal Model:U87MG-EGFR subcutaneous and intracranial xenograft model[2]
Dosage:5 mg/kg
Administration:Intraperitoneal injection (i.p.), once a day for 10 days
Result:Decreased the luminescence intensity of brain tumor about 70%.
Decreased p-EGFR, p-STAT3, and p-Akt levels in the intracranial tumors as compared with the vehicles.
Decreased Ki67 and active caspase-3 immunostaining of intracranial tumors.

References

[1] S TOMA. Metabolism and pharmacokinetics of cinobufagin.[J]. Xenobiotica, 1987, 17 10: 1195-1202. DOI: 10.3109/00498258709167411
[2] XING-SHENG LU. Preclinical study of cinobufagin as a promising anti-colorectal cancer agent[J]. Oncotarget, 2016, 8 1: 988-998. DOI: 10.18632/oncotarget.13519
[3] SHANSHAN XIE. Cinobufagin Modulates Human Innate Immune Responses and Triggers Antibacterial Activity.[J]. ACS Applied Bio Materials, 2016: e0160734. DOI: 10.1371/journal.pone.0160734
[4] LONGSHENG XU. Alpha-7 Nicotinic Receptor-Targeted Cinobufagin Induces Antinociception and Inhibits NF-κB Signaling Pathway in DRG Neurons[J]. ACS Chemical Neuroscience, 2018, 10 1: 497-506. DOI: 10.1021/acschemneuro.8b00369

Cinobufagin Preparation Products And Raw materials

Raw materials

Preparation Products

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Cinobufagin Suppliers

Nanjing Endesa Bio-tech Co., Ltd.
Tel
025-58862853 18915922038
Fax
+86-025-58862853
Email
sales@endesachem.com
Country
China
ProdList
591
Advantage
58
J & K SCIENTIFIC LTD.
Tel
18210857532; 18210857532
Fax
86-10-82849933
Email
jkinfo@jkchemical.com
Country
China
ProdList
96815
Advantage
76
Chembest Research Laboratories Limited
Tel
+86-21-20908456
Fax
021-58180499
Email
sales@BioChemBest.com
Country
China
ProdList
6003
Advantage
61
TCI (Shanghai) Development Co., Ltd.
Tel
021-67121386
Fax
021-67121385
Email
Sales-CN@TCIchemicals.com
Country
China
ProdList
24529
Advantage
81
Chemsky(shanghai)International Co.,Ltd.
Tel
021-50135380
Email
shchemsky@sina.com
Country
China
ProdList
32321
Advantage
50
Aktin Chemicals, Inc.
Tel
86-28-85159085
Fax
86-28-85152372
Email
info@aktinchem.com
Country
China
ProdList
297
Advantage
62
Dalian Meilun Biotech Co., Ltd.
Tel
0411-62910999 13889544652
Email
sales@meilune.com
Country
China
ProdList
4747
Advantage
58
BioBioPha Co., Ltd.
Tel
0871-65217109 13211707573;
Fax
0871-65215563
Email
y.liu@mail.biobiopha.com
Country
China
ProdList
5653
Advantage
65
ShangHai YuanYe Biotechnology Co., Ltd.
Tel
021-61312847 13636370518
Fax
021-55068248
Email
shyysw007@163.com
Country
China
ProdList
4999
Advantage
60
Chengdu Biopurify Phytochemicals Ltd.
Tel
+86-028-82633397 18982077548
Fax
+86-28-82633165
Email
cwb1@biopurify.cn
Country
China
ProdList
2376
Advantage
60
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View Lastest Price from Cinobufagin manufacturers

Shaanxi Dideu Medichem Co. Ltd
Product
Cinobufagin 470-37-1
Price
US $1.00-100.00/KG
Min. Order
1KG
Purity
95%
Supply Ability
300KG
Release date
2024-07-26
Shanghai Standard Technology Co., Ltd.
Product
Cinobufagin 470-37-1
Price
US $0.00/mg
Min. Order
5mg
Purity
Analytical Standard
Supply Ability
10 g
Release date
2019-10-10
Shanghai Standard Technology Co., Ltd.
Product
Cinobufagin 470-37-1
Price
US $0.00/mg
Min. Order
5mg
Purity
≥98%(HPLC)
Supply Ability
10 g
Release date
2019-10-10

470-37-1, CinobufaginRelated Search:


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