Methylene Blue

ChemicalBook > CAS DataBase List > Methylene Blue

Product Name: Methylene Blue
CAS No.: 61-73-4
Chemical Name: Methylene Blue
Synonyms: METHYLTHIONINIUM CHLORIDE;BASIC BLUE 9;abcolmethyleneblue2b;METHYLTHIONINE CHLORIDE;METHYLENE BLUE SOLUTION;Methylthioninium chloride CRS;ue;ene BL;CI 52015;70.0%(W)
CBNumber: CB2748858
Molecular Formula: C16H18ClN3S
Formula Weight: 319.85
MOL File: 61-73-4.mol

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Methylene Blue Property

Melting point:: 190 °C (dec.)(lit.)
Density: 1.0 g/mL at 20 °C
refractive index: n20/D1.347
Flash point:: 45 °C
storage temp.: room temp
solubility: Soluble in water, ethanol, ethylene glycol, methyl cellosolve
form: Liquid
pka: 2.6, 11.2(at 25℃)
Colour Index: 52015
color: Green
Specific Gravity: 0.98
Odor: Odorless
Water Solubility: 40 g/L (20 ºC)
λmax: 661 nm
Merck: 14,6060
BRN: 3641570
Biological Applications: Detecting microorganisms; treating diabetic retinopathy,macular degeneration,malignant uveal melanomas,erysipelas,hidradenitis suppurativa,inflammation,skin diseases
NIST Chemistry Reference: Methylene blue(61-73-4)
EPA Substance Registry System: Methylene blue (61-73-4)

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Safety

Hazard Codes: Xn
Risk Statements: 10-41-22-20/21/22-36/37/38
Safety Statements: 26-39-36
RIDADR: UN 1993 3/PG 3
WGK Germany: 3
RTECS: SP5740000
HS Code: 32049010
Hazardous Substances Data: 61-73-4(Hazardous Substances Data)
Toxicity: LD50 oral in rat: 1180mg/kg

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Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Warning

Hazard statements

H302Harmful if swallowed

Precautionary statements

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P270Do not eat, drink or smoke when using this product.

P301+P312IF SWALLOWED: call a POISON CENTER or doctor/physician IF you feel unwell.

P501Dispose of contents/container to..…

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N-Bromosuccinimide Price

Sigma-Aldrich

Product number: 457250
Product name: Methylene Blue - CAS 61-73-4 - Calbiochem
Purity: Inhibitor of soluble guanylate cyclase.
Packaging: 1g
Price: $56.2
Updated: 2024/03/01

Sigma-Aldrich

Product number: 319112
Product name: Methylene Blue solution
Purity: 0.05?wt.%inH2O
Packaging: 100mL
Price: $25
Updated: 2024/03/01

Sigma-Aldrich

Product number: 1808
Product name: Methylene Blue solution
Purity: 1.4?%(w/v)in95%ethanol
Packaging: 50mL
Price: $59.8
Updated: 2024/03/01

Sigma-Aldrich

Product number: 03978
Product name: Methylene Blue solution
Packaging: 250ml
Price: $53.7
Updated: 2024/03/01

Sigma-Aldrich

Product number: 284-M
Product name: Methylene Blue Chloride
Purity: HARLECO?,suitableforhistology
Packaging: 25g
Price: $108
Updated: 2023/01/07

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Methylene Blue Chemical Properties,Usage,Production

Chemical Properties

Methylene blue is a heterocyclic aromatic chemical compound with the molecular formula C16H18N3SCl. It has many uses in a range of different fields, such as biology and chemistry. At room temperature it appears as a solid, odorless, dark green powder, that yields a blue solution when dissolved in water. The hydrated form has 3 molecules of water per molecule of methylene blue. Methylene blue should not be confused with methyl blue, another histology stain, new methylene blue, nor with the methyl violets often used as pH indicators.

Uses

A useful inhibitor of cGMP-mediated processes and a useful stain

Uses

Methylene Blue, 1% w/v aqueous solution is used as a dye in different staining procedures viz. Wright's stain and Jenner's stain. It also serves as an indicator and medicine. Further, it is used to examine RNA and DNA under the microscope or in a gel. It is widely used as a redox indicator in analytical chemistry. It is also used in the sulfide analysis.

Definition

ChEBI: An organic chloride salt having 3,7-bis(dimethylamino)phenothiazin-5-ium as the counterion. A commonly used dye that also exhibits antioxidant, antimalarial, antidepressant and cardioprotective properties.

Preparation

MethyleneBlue45,C.I. 52 015 [61-73-4], is obtained by oxidation of 4-aminodimethylaniline in the presence ofsodium thiosulfate to give the quinonediiminothiosulfonic acid, reaction with dimethylaniline, oxidation to the indamine, and cyclization to give the thiazine.

General Description

Methylene blue, also known as methylthioninium chloride, is a photoactive phenothiazine dye. It is a small molecular weight dye, which is effective against Gram positive and Gram negative bacteria.

Hazard

Toxic by ingestion.

Biological Activity

Biological stain and redox indicator. Inhibits tau filament formation (IC 50 = 1.9 μ M). Also inhibits soluble guanylyl cyclase.

Biochem/physiol Actions

Methylene blue (MB) is a highly recognized histological dye. It plays a vital role in microbiology and pharmacology. It has been employed in staining living organisms and in methemoglobinemia treatment. MB exhibits maximum absorption at 664nm. Exposure to methylene blue leads to various health hazards such as increased heart rate, vomiting, shock, Heinz body formation, cyanosis, jaundice, quadriplegia and tissue necrosis in humans

Clinical Use

Methylene blue is 3,7-bis(dimethylamino)-phenazathioniumchloride (Urised). The compound occurs as a dark greencrystalline powder with a metallic appearance that is solublein water (1:25) and alcohol (1:65).
Methylene blue has weak antiseptic properties that makeit useful for the treatment of cystitis and urethritis. The actionof methylene blue is considered to be bacteriostatic.The compound colors the urine and stool blue green.

Safety Profile

Poison by ingestion,intraperitoneal, intravenous, and subcutaneous routes.Human systemic effects: cyanosis, blood changes.Experimental reproductive effects. Mutation datareported. When heated to decomposition it emits verytoxic fumes of NOx, SOx, and

Veterinary Drugs and Treatments

Methylene blue is used primarily for treating methemoglobinemia secondary to oxidative agents (nitrates, chlorates) in ruminants. It is also employed occasionally as adjunctive or alternative therapy for cyanide toxicity.
Intra-operative methylene blue is also being used to preferentially stain islet-cell tumors of the pancreas in dogs in order to aid in their surgical removal or in determining the animal’s prognosis.

Purification Methods

Crystallise the chloride from 0.1M 664 2HCl (16mL/g), the crystals are separated by centrifugation, washed with chilled EtOH and diethyl ether, and dried under vacuum. Crystallise it from 50% aqueous EtOH, wash it with absolute EtOH, and dry it at 50-55o for 24hours. It has also been crystallised from *benzene/MeOH (3:1). It has been salted out with NaCl from a commercial concentrated aqueous solution, then crystallised from water, and dried at 100o in an oven for 8-10hours. [Beilstein 27 III/IV 5152.]

Methylene Blue Preparation Products And Raw materials

Raw materials

Sodium dichromate dihydrate N-Methylaniline thiazine Chromic acid N,N-Dimethylaniline Aluminium sulfate hydrate N,N-Dimethyl-1,4-phenylenediamine

Preparation Products

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View Lastest Price from Methylene Blue manufacturers

Hebei Zhuanglai Chemical Trading Co.,Ltd

Product: Methylene Blue 61-73-4
Price: US $40.00/kg
Min. Order: 1kg
Purity: 99%
Supply Ability: 5000kg/week
Release date: 2024-05-17

Henan Fengda Chemical Co., Ltd

Product: Methylene Blue 61-73-4
Price: US $8.00-0.90/KG
Min. Order: 1KG
Purity: 99%
Supply Ability: g-kg-tons, free sample is available
Release date: 2024-03-28

Hebei Dongdu Import and Export Co. LTD

Product: methylene blue 61-73-4
Price: US $56.00/kg
Min. Order: 1kg
Purity: 99%
Supply Ability: 10000ton
Release date: 2023-12-21

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