Alkaline Phosphatase
- Product Name
- Alkaline Phosphatase
- CAS No.
- 9001-78-9
- Chemical Name
- Alkaline Phosphatase
- Synonyms
- AP;alkaline;MCAP;CIAP;AKP;IRAP;IL1F3;HUNK1;HUNKI;IL-1RN
- CBNumber
- CB9690084
- Molecular Formula
- C21H36N8O6
- Formula Weight
- 496.56054
- MOL File
- 9001-78-9.mol
Alkaline Phosphatase Property
- Density
- 1.12 g/mL at 20 °C
- storage temp.
- -20°C
- form
- suspension
- color
- white
- Specific Gravity
- 1
- Water Solubility
- It is soluble in water.
- Stability:
- Stability Incompatible with strong oxidizing agents
- EPA Substance Registry System
- Alkaline phosphatase (9001-78-9)
Safety
- Hazard Codes
- B,Xi
- Risk Statements
- 36/37/38
- Safety Statements
- 26-36-24/25-22-23
- WGK Germany
- 3
- F
- 3-10
- TSCA
- Yes
- HS Code
- 35079090
- Toxicity
- An enzyme with an alkaline pH optimum that hydrolyzes phosphate monoesters. Elevation of its activity in the serum usually indicates obstructive jaundice, Paget’s disease (osteitis deformans), or bone carcinoma.
N-Bromosuccinimide Price
- Product number
- SAE0111
- Product name
- Phosphatase, Alkaline from human placenta
- Purity
- ≥10?units/mg solid (in glycine buffer)
- Packaging
- 100UNITS
- Price
- $147
- Updated
- 2024/03/01
- Product number
- SRP3327
- Product name
- IL-1 RECEPTOR ANTAGONIST human
- Purity
- Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)
- Packaging
- 100μG
- Price
- $412
- Updated
- 2024/03/01
- Product number
- SAE0111
- Product name
- Phosphatase, Alkaline from human placenta
- Purity
- ≥10?units/mg solid (in glycine buffer)
- Packaging
- 1000UNITS
- Price
- $611
- Updated
- 2024/03/01
- Product number
- P8361
- Product name
- Phosphatase, Alkaline bovine
- Purity
- recombinant, expressed in Pichia pastoris, ≥4000?units/mg protein
- Packaging
- 5000units
- Price
- $521
- Updated
- 2024/03/01
- Product number
- P7923
- Product name
- Phosphatase, Alkaline from bovine intestinal mucosa
- Purity
- buffered aqueous glycerol solution, ≥4,000?DEA units/mg protein
- Packaging
- 2000units
- Price
- $178
- Updated
- 2024/03/01
Alkaline Phosphatase Chemical Properties,Usage,Production
Chemical Properties
solid
Uses
Hydrolyzes 5′-terminal monophosphate (dephosphorylation) from DNA and RNA. Prevents fragments from self annealing. 5′-nucleic acid targeting for probes.
Uses
Alkaline Phosphatase, calf intestine, EIA GradeDephosphorylation of cloning vector DNA to prevent recircularization during ligation, dephosphorylation of DNA prior to end-labeling using T4 Polynucleotide Kinase, treatment of dNTPs in PCR reactions prior to sequencing or SNP analysis, dephosphorylation of DNA and RNA. It is also used to remove the 5?-terminal phosphate from nucleic acids during molecular cloning reactions.
Uses
Alkaline Phosphatase is commonly used to remove the 5′-terminal phosphate from nucleic acids.
General Description
Alkaline phosphatase from bovine intestinal mucosa is both a phosphomonoesterase and a pyrophosphatase.
Biochem/physiol Actions
Alkaline phosphatase is a model enzyme for understanding phosphomonoesterase. It is used in various biochemical methods and enzyme linked immunosorbent assay (ELISA).
Purification Methods
The E.coli supernatant in sucrose (20%, 33mM) in Tris-HCl pH 8.0 is purified through a DEAE-cellulose column and recrystallised. To the column eluates in 0.125M NaCl is added MgCl2 (to 0.01M) and brought to 50% saturation in (NH4)2SO4 by adding the solid (0.20g/mL). The mixture is centrifuged to remove bubbles and is adjusted to pH 8.0 (with 2N NaOH). Saturated (NH4)2SO4 at pH 8.0 is added dropwise until the solution becomes faintly turbid (~61% saturation). It is set aside at ~25o for 1hour (turbidity will increase). The mixture is placed in an ice bath for several minutes when turbidity disappears and a clear solution is obtained. It is then placed in a large ice bath at 0o (~5L) and allowed to warm slowly to room temperature in a dark room whereby crystals are formed appearing as a silky sheen. The crystals are collected by centrifugation at 25o if necessary. The crystalline solutions are stable at ~25o for many months. They can be stored at 0o, but are unstable when frozen. Cysteine at 10-3M and thioglycolic acid at 10-4M are inhibitory. This is reversed on addition of Zn2+ ions. Many organic phosphates are good substrates for this phosphatase. [Molamy & Horecker Methods Enzymol 9 639 1966, Torriani et al. Methods Enzymol 12b 212 1968, Engstrom Biochim Biophys Acta 92 71 1964.] Alkaline phosphatase from rat osteosarcoma has been purified by Me2CO precipitation and chromatography on DEAE-cellulose, Sephacryl S-200, and hydroxylapatite. [Nair et al. Arch Biochem Biophys 254 18 1987.] Phosphoproteins (various). These are purified by adsorbing onto an iminodiacetic acid substituted agarose column to which are bound ferric ions. This chelate complex acts as a selective immobilised metal affinity adsorbent for phosphoproteins. [Muszyfiska et al. Biochemistry 25 6850 1986.]
Alkaline Phosphatase Preparation Products And Raw materials
Raw materials
Preparation Products
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View Lastest Price from Alkaline Phosphatase manufacturers
- Product
- Alkaline Phosphatase 9001-78-9
- Price
- US $0.00-0.00/g
- Min. Order
- 1g
- Purity
- 99% HPLC
- Supply Ability
- 100kg
- Release date
- 2023-06-30
- Product
- Phosphatase, Alkaline froM Calf Intestinal Mucosa 9001-78-9
- Price
- US $1.00-0.00/KG
- Min. Order
- 1KG
- Purity
- 99%
- Supply Ability
- 200000KG
- Release date
- 2020-01-08
- Product
- Alkaline Phosphatase 9001-78-9
- Price
- US $1.00/KG
- Min. Order
- 1KG
- Purity
- 99%
- Supply Ability
- 25kg
- Release date
- 2019-07-03