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Alkaline Phosphatase

Product Name
Alkaline Phosphatase
CAS No.
9001-78-9
Chemical Name
Alkaline Phosphatase
Synonyms
AP;alkaline;MCAP;CIAP;AKP;IRAP;IL1F3;HUNK1;HUNKI;Apase
CBNumber
CB9690084
Molecular Formula
C21H36N8O6
Formula Weight
496.56054
MOL File
9001-78-9.mol
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Alkaline Phosphatase Property

Density 
1.12 g/mL at 20 °C
storage temp. 
-20°C
form 
suspension
color 
white
Specific Gravity
1
Water Solubility 
It is soluble in water.
Stability:
Stability Incompatible with strong oxidizing agents
EPA Substance Registry System
Alkaline phosphatase (9001-78-9)
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Safety

Hazard Codes 
B,Xi
Risk Statements 
36/37/38
Safety Statements 
26-36-24/25-22-23
WGK Germany 
3
F 
3-10
TSCA 
Yes
HS Code 
35079090
Toxicity
An enzyme with an alkaline pH optimum that hydrolyzes phosphate monoesters. Elevation of its activity in the serum usually indicates obstructive jaundice, Paget’s disease (osteitis deformans), or bone carcinoma.
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Warning
Hazard statements

H317May cause an allergic skin reaction

Precautionary statements

P280Wear protective gloves/protective clothing/eye protection/face protection.

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
SAE0111
Product name
Phosphatase, Alkaline from human placenta
Purity
≥10?units/mg solid (in glycine buffer)
Packaging
100UNITS
Price
$147
Updated
2024/03/01
Sigma-Aldrich
Product number
SRP3327
Product name
IL-1 RECEPTOR ANTAGONIST human
Purity
Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)
Packaging
100μG
Price
$412
Updated
2024/03/01
Sigma-Aldrich
Product number
SAE0111
Product name
Phosphatase, Alkaline from human placenta
Purity
≥10?units/mg solid (in glycine buffer)
Packaging
1000UNITS
Price
$611
Updated
2024/03/01
Sigma-Aldrich
Product number
P8361
Product name
Phosphatase, Alkaline bovine
Purity
recombinant, expressed in Pichia pastoris, ≥4000?units/mg protein
Packaging
5000units
Price
$521
Updated
2024/03/01
Sigma-Aldrich
Product number
P7923
Product name
Phosphatase, Alkaline from bovine intestinal mucosa
Purity
buffered aqueous glycerol solution, ≥4,000?DEA units/mg protein
Packaging
2000units
Price
$178
Updated
2024/03/01
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Alkaline Phosphatase Chemical Properties,Usage,Production

Chemical Properties

solid

Uses

Hydrolyzes 5′-terminal monophosphate (dephosphorylation) from DNA and RNA. Prevents fragments from self annealing. 5′-nucleic acid targeting for probes.

Uses

Alkaline Phosphatase, calf intestine, EIA GradeDephosphorylation of cloning vector DNA to prevent recircularization during ligation, dephosphorylation of DNA prior to end-labeling using T4 Polynucleotide Kinase, treatment of dNTPs in PCR reactions prior to sequencing or SNP analysis, dephosphorylation of DNA and RNA. It is also used to remove the 5?-terminal phosphate from nucleic acids during molecular cloning reactions.

Uses

Alkaline Phosphatase is commonly used to remove the 5′-terminal phosphate from nucleic acids.

General Description

Alkaline phosphatase from bovine intestinal mucosa is both a phosphomonoesterase and a pyrophosphatase.

Biochem/physiol Actions

Alkaline phosphatase is a model enzyme for understanding phosphomonoesterase. It is used in various biochemical methods and enzyme linked immunosorbent assay (ELISA).

Purification Methods

The E.coli supernatant in sucrose (20%, 33mM) in Tris-HCl pH 8.0 is purified through a DEAE-cellulose column and recrystallised. To the column eluates in 0.125M NaCl is added MgCl2 (to 0.01M) and brought to 50% saturation in (NH4)2SO4 by adding the solid (0.20g/mL). The mixture is centrifuged to remove bubbles and is adjusted to pH 8.0 (with 2N NaOH). Saturated (NH4)2SO4 at pH 8.0 is added dropwise until the solution becomes faintly turbid (~61% saturation). It is set aside at ~25o for 1hour (turbidity will increase). The mixture is placed in an ice bath for several minutes when turbidity disappears and a clear solution is obtained. It is then placed in a large ice bath at 0o (~5L) and allowed to warm slowly to room temperature in a dark room whereby crystals are formed appearing as a silky sheen. The crystals are collected by centrifugation at 25o if necessary. The crystalline solutions are stable at ~25o for many months. They can be stored at 0o, but are unstable when frozen. Cysteine at 10-3M and thioglycolic acid at 10-4M are inhibitory. This is reversed on addition of Zn2+ ions. Many organic phosphates are good substrates for this phosphatase. [Molamy & Horecker Methods Enzymol 9 639 1966, Torriani et al. Methods Enzymol 12b 212 1968, Engstrom Biochim Biophys Acta 92 71 1964.] Alkaline phosphatase from rat osteosarcoma has been purified by Me2CO precipitation and chromatography on DEAE-cellulose, Sephacryl S-200, and hydroxylapatite. [Nair et al. Arch Biochem Biophys 254 18 1987.] Phosphoproteins (various). These are purified by adsorbing onto an iminodiacetic acid substituted agarose column to which are bound ferric ions. This chelate complex acts as a selective immobilised metal affinity adsorbent for phosphoproteins. [Muszyfiska et al. Biochemistry 25 6850 1986.]

Alkaline Phosphatase Preparation Products And Raw materials

Raw materials

1-Butanol

Preparation Products

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View Lastest Price from Alkaline Phosphatase manufacturers

Shaanxi Dideu Medichem Co. Ltd
Product
Phosphatase, Alkaline froM Calf Intestinal Mucosa 9001-78-9
Price
US $1.00-0.00/KG
Min. Order
1KG
Purity
99%
Supply Ability
200000KG
Release date
2020-01-08
Hangzhou ICH Biofarm Co., Ltd
Product
Alkaline Phosphatase 9001-78-9
Price
US $0.00-0.00/g
Min. Order
1g
Purity
99% HPLC
Supply Ability
100kg
Release date
2023-06-30
Career Henan Chemical Co
Product
Alkaline Phosphatase 9001-78-9
Price
US $1.00/KG
Min. Order
1KG
Purity
99%
Supply Ability
25kg
Release date
2019-07-03

9001-78-9, Alkaline PhosphataseRelated Search:

Magnesium carbonate hydroxide FUCHSIN BASIC Cupric acetylacetonate Ferric acetylacetonate PHENYLSELENOL COBALT(II) ACETYLACETONATE 2,4-PENTANEDIONE, SILVER DERIVATIVE TERT-BUTYL ISOCYANIDE Tosylmethyl isocyanide N-BUTYLISOCYANIDE SALCOMINE TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)DYSPROSIUM(III) Tris(2,4-pentanedionato)chroMiuM(III) Aluminum acetylacetonate DICHLORO(ETHYLENEDIAMINE)PLATINUM(II) 1,1,3,3-TETRAMETHYLBUTYL ISOCYANIDE METHYL ISOCYANOACETATE 3-INDOXYL PHOSPHATE DISODIUM SALT

  • AlkalinePhosphatase,(AmmoniumSulfateSuspensionCalfIntestine)
  • ALKALINE PHOSPHATASE, RECOMBINANT, MOLECULAR BIOLOGY GRADE
  • ALKALINE PHOSPHATASE (AP), ORIGIN BOVINE INTESTINE, RECOMBINANT
  • Alkaline Phosphatase from calf intestine ca. 3 000 U/mg protein (ca. 60 U/
  • Alkaline Phosphatase from calf intestine ca. 5 400 U/mg protein
  • Alkaline Phosphatase from calf intestine ca. 7000 U/mg protein
  • alkaline phosphatase from calf intestinal mucosa
  • phosphatase, alkaline from escherichia coli
  • phosphatase, alkaline from bovine liver
  • phosphatase alkaline from calf intestinal mucosa
  • phosphatase, alkaline from porcine intestinal mucosa
  • phosphatase, alkaline from rabbit intestine
  • phosphatase, alkaline from human placenta
  • PHOSPHORIC MONOESTER PHOSPHOHYDROLASE [ALKALINE OPTIMUM] TYPE III-L
  • PHOSPHORIC MONOESTER PHOSPHOHYDROLASE [ALKALINE OPTIMUM] TYPE III-S
  • PHOSPHORIC MONOESTER PHOSPHOHYDROLASE [ALKALINE OPTIMUM] TYPE I-S
  • PHOSPHORIC MONOESTER PHOSPHOHYDROLASE [ALKALINE OPTIMUM] TYPE IV
  • PHOSPHORIC MONOESTER PHOSPHOHYDROLASE [ALKALINE OPTIMUM] TYPE VIII
  • PHOSPHORIC MONOESTER PHOSPHOHYDROLASE [ALKALINE OPTIMUM] TYPE VII-L
  • PHOSPHORIC MONOESTER PHOSPHOHYDROLASE [ALKALINE OPTIMUM] TYPE VII-S
  • PHOSPHORIC MONOESTER PHOSPHOHYDROLASE [ALKALINE OPTIMUM] TYPE VII-T
  • PHOSPHORIC MONOESTER PHOSPHOHYDROLASE [ALKALINE OPTIMUM] TYPE XVII
  • PHOSPHORIC MONOESTER PHOSPHOHYDROLASE [ALKALINE OPTIMUM] TYPE XXIV
  • PHOSPHORIC MONOESTER PHOSPHOHYDROLASE [ALKALINE OPTIMUM] TYPE XXXII
  • PHOSPHORIC MONOESTER PHOSPHOHYDROLASE [ALKALINE OPTIMUM] TYPE XXXIII
  • PHOSPHATASE, ALKALINE BOVINE
  • PHOSPHATASE, ALKALINE CALF INTESTINE
  • PHOSPHATASE, ALKALINE, E COLI
  • PHOSPHATASE, ALKALINE, HUMAN PLACENTA
  • PHOSPHATASE, ALKALINE TYPE III
  • PHOSPHATASE, ALKALINE TYPE III-L
  • PHOSPHATASE, ALKALINE TYPE III-S
  • PHOSPHATASE, ALKALINE TYPE I-S
  • PHOSPHATASE, ALKALINE TYPE IV
  • PHOSPHATASE, ALKALINE TYPE VIII
  • PHOSPHATASE, ALKALINE TYPE VII-L
  • PHOSPHATASE, ALKALINE TYPE VII-S
  • PHOSPHATASE, ALKALINE TYPE VII-T
  • PHOSPHATASE, ALKALINE TYPE XVII
  • PHOSPHATASE, ALKALINE TYPE XXIV
  • PHOSPHATASE, ALKALINE TYPE XXXII
  • PHOSPHATASE, ALKALINE TYPE XXXIII
  • PHOSPHATASE, ALKALINE
  • PHOSPHTASE ALKALINE
  • Alkaline phosphatase (catalytic concentration)
  • phosphatase alk. from calf intestinal mucosa
  • Phosphatase alkaline bovine intestinal mucosa
  • Phosphatase alkaline calf intestinal mucosa
  • Alkaline Phosphatase from E. coli
  • Phosphatase, Alkaline from bovine milk
  • Phosphatase alkaline, immobilized on Agarose from calf intestinal mucosa
  • Phosphatase, Alkaline from canine intestine
  • AKP
  • phosphomonesterase, alkaline, calf intestinal mucosa
  • ALKALINE PHOSPHATASE SOLUTION, FROM CALF INTESTINE
  • PHOSPHATASE, ALKALINE BACTERIAL (BAP)
  • PHOSPHATASE, ALKALINE FROM CALF INTESTINE (CIP)
  • 5-Allyl-5-(2-hydroxypropyl)-1-phenyl-2,4,6(1H,3H,5H)-pyrimidinetrione