ChemicalBook > CAS DataBase List > Alkaline Phosphatase

Alkaline Phosphatase

Description Source

Product Name: Alkaline Phosphatase
CAS No.: 9001-78-9
Chemical Name: Alkaline Phosphatase
Synonyms: AP;alkaline;MCAP;CIAP;AKP;IRAP;IL1F3;HUNK1;HUNKI;Apase
CBNumber: CB9690084
Molecular Formula: C21H36N8O6
Formula Weight: 496.56054
MOL File: 9001-78-9.mol

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Alkaline Phosphatase Property

Density: 1.12 g/mL at 20 °C
storage temp.: -20°C
form: suspension
color: white
Specific Gravity: 1
PH: ~7.6
biological source: mouse
Water Solubility: It is soluble in water.
Specific Activity: 30-90units/mg protein (modified Warburg-Christian, in glycine buffer)
Stability:: Stability Incompatible with strong oxidizing agents
InChIKey: ITZMJCSORYKOSI-XUAWLZQMNA-N
SMILES: C(O)(=O)[C@@H](NC([C@@H]1CCCN1C(CNC([C@@H]1CCCN1C(=O)[C@@H](N)C)=O)=O)=O)CCC/N=C(\N)/N |&1:3,6,15,22,r|
EPA Substance Registry System: Alkaline phosphatase (9001-78-9)
Absorption: 7.6 at 278nm (10 mg AP/ml)

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Safety

Hazard Codes: B,Xi
Risk Statements: 36/37/38
Safety Statements: 26-36-24/25-22-23
WGK Germany: 3
F: 3-10
TSCA: Yes
HS Code: 35079090
Toxicity: An enzyme with an alkaline pH optimum that hydrolyzes phosphate monoesters. Elevation of its activity in the serum usually indicates obstructive jaundice, Paget’s disease (osteitis deformans), or bone carcinoma.

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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word: Warning
Hazard statements: H317May cause an allergic skin reaction
Precautionary statements: P280Wear protective gloves/protective clothing/eye protection/face protection.

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N-Bromosuccinimide Price

Sigma-Aldrich

Product number: SRP6006
Product name: IL-1RA murine
Purity: recombinant, expressed in E. coli, ≥98% (SDS-PAGE)
Packaging: 50 μg
Price: $460
Updated: 2025/07/31

Sigma-Aldrich

Product number: SRP3327
Product name: IL-1 RECEPTOR ANTAGONIST human
Purity: Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)
Packaging: 100μG
Price: $425
Updated: 2025/07/31

Sigma-Aldrich

Product number: SRP0464
Product name: BRD4, BD1, BD2 (49-460) His tag human
Purity: recombinant, expressed in E. coli, ≥75% (SDS-PAGE)
Packaging: 100 μg
Price: $707
Updated: 2025/07/31

Sigma-Aldrich

Product number: SRP0463
Product name: BRD4, BD1, BD2 (49-460) GST tag human
Purity: recombinant, expressed in E. coli, ≥80% (SDS-PAGE)
Packaging: 100 μg
Price: $710
Updated: 2025/07/31

Sigma-Aldrich

Product number: SRP0462
Product name: BRD4 (342-460) His tag human
Purity: recombinant, expressed in E. coli, ≥90% (SDS-PAGE)
Packaging: 100 μg
Price: $710
Updated: 2025/07/31

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Alkaline Phosphatase Chemical Properties,Usage,Production

Chemical Properties

solid

Uses

Hydrolyzes 5′-terminal monophosphate (dephosphorylation) from DNA and RNA. Prevents fragments from self annealing. 5′-nucleic acid targeting for probes.

Uses

Alkaline Phosphatase, calf intestine, EIA GradeDephosphorylation of cloning vector DNA to prevent recircularization during ligation, dephosphorylation of DNA prior to end-labeling using T4 Polynucleotide Kinase, treatment of dNTPs in PCR reactions prior to sequencing or SNP analysis, dephosphorylation of DNA and RNA. It is also used to remove the 5?-terminal phosphate from nucleic acids during molecular cloning reactions.

Uses

Alkaline Phosphatase is commonly used to remove the 5′-terminal phosphate from nucleic acids.

General Description

Alkaline phosphatase from bovine intestinal mucosa is both a phosphomonoesterase and a pyrophosphatase.

Biochem/physiol Actions

Alkaline phosphatase is a model enzyme for understanding phosphomonoesterase. It is used in various biochemical methods and enzyme linked immunosorbent assay (ELISA).

Purification Methods

The E.coli supernatant in sucrose (20%, 33mM) in Tris-HCl pH 8.0 is purified through a DEAE-cellulose column and recrystallised. To the column eluates in 0.125M NaCl is added MgCl2 (to 0.01M) and brought to 50% saturation in (NH4)2SO4 by adding the solid (0.20g/mL). The mixture is centrifuged to remove bubbles and is adjusted to pH 8.0 (with 2N NaOH). Saturated (NH4)2SO4 at pH 8.0 is added dropwise until the solution becomes faintly turbid (~61% saturation). It is set aside at ~25o for 1hour (turbidity will increase). The mixture is placed in an ice bath for several minutes when turbidity disappears and a clear solution is obtained. It is then placed in a large ice bath at 0o (~5L) and allowed to warm slowly to room temperature in a dark room whereby crystals are formed appearing as a silky sheen. The crystals are collected by centrifugation at 25o if necessary. The crystalline solutions are stable at ~25o for many months. They can be stored at 0o, but are unstable when frozen. Cysteine at 10-3M and thioglycolic acid at 10-4M are inhibitory. This is reversed on addition of Zn2+ ions. Many organic phosphates are good substrates for this phosphatase. [Molamy & Horecker Methods Enzymol 9 639 1966, Torriani et al. Methods Enzymol 12b 212 1968, Engstrom Biochim Biophys Acta 92 71 1964.] Alkaline phosphatase from rat osteosarcoma has been purified by Me2CO precipitation and chromatography on DEAE-cellulose, Sephacryl S-200, and hydroxylapatite. [Nair et al. Arch Biochem Biophys 254 18 1987.] Phosphoproteins (various). These are purified by adsorbing onto an iminodiacetic acid substituted agarose column to which are bound ferric ions. This chelate complex acts as a selective immobilised metal affinity adsorbent for phosphoproteins. [Muszyfiska et al. Biochemistry 25 6850 1986.]

Description

The Alkaline Phosphatase is purified by affinity chromatography, which results in an enzyme of high specific activity and purity.

Alkaline Phosphatase is Dimeric protein having a molecular weight of 140 kDa, one Zn++ ion is tightly bound to each subunit, and another less tightly bound is involved in the catalytic reaction. Mg++ stimulates the catalysis. The binding site for Mg++ is different to that of Zn++, but will be occupied by excess Zn++ followed by loss of enzyme activity.

Source

Calf Intestine

Alkaline Phosphatase Preparation Products And Raw materials

Raw materials

1-Butanol

Preparation Products

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View Lastest Price from Alkaline Phosphatase manufacturers

Shaanxi Dideu Medichem Co. Ltd

Product: Alkaline Phosphatase 9001-78-9
Price: US $0.10/KG
Min. Order: 1KG
Purity: 99.0%
Supply Ability: 1000 tons
Release date: 2024-08-30

Shaanxi Dideu Medichem Co. Ltd

Product: Phosphatase, Alkaline froM Calf Intestinal Mucosa 9001-78-9
Price: US $1.00-0.00/KG
Min. Order: 1KG
Purity: 99%
Supply Ability: 200000KG
Release date: 2020-01-08

Hangzhou ICH Biofarm Co., Ltd

Product: Alkaline Phosphatase 9001-78-9
Price: US $0.00-0.00/g
Min. Order: 1g
Purity: 99% HPLC
Supply Ability: 100kg
Release date: 2023-06-30

9001-78-9, Alkaline PhosphataseRelated Search:

Magnesium carbonate hydroxide FUCHSIN BASIC Cupric acetylacetonate Ferric acetylacetonate PHENYLSELENOL COBALT(II) ACETYLACETONATE 2,4-PENTANEDIONE, SILVER DERIVATIVE TERT-BUTYL ISOCYANIDE Tosylmethyl isocyanide N-BUTYLISOCYANIDE SALCOMINE TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)DYSPROSIUM(III) Tris(2,4-pentanedionato)chroMiuM(III) Aluminum acetylacetonate DICHLORO(ETHYLENEDIAMINE)PLATINUM(II) 1,1,3,3-TETRAMETHYLBUTYL ISOCYANIDE METHYL ISOCYANOACETATE 3-INDOXYL PHOSPHATE DISODIUM SALT

AlkalinePhosphatase,(AmmoniumSulfateSuspensionCalfIntestine)

ALKALINE PHOSPHATASE, RECOMBINANT, MOLECULAR BIOLOGY GRADE

ALKALINE PHOSPHATASE (AP), ORIGIN BOVINE INTESTINE, RECOMBINANT

Alkaline Phosphatase from calf intestine ca. 3 000 U/mg protein (ca. 60 U/

Alkaline Phosphatase from calf intestine ca. 5 400 U/mg protein

Alkaline Phosphatase from calf intestine ca. 7000 U/mg protein

alkaline phosphatase from calf intestinal mucosa

phosphatase, alkaline from escherichia coli

phosphatase, alkaline from bovine liver

phosphatase alkaline from calf intestinal mucosa

phosphatase, alkaline from porcine intestinal mucosa

phosphatase, alkaline from rabbit intestine

phosphatase, alkaline from human placenta

PHOSPHORIC MONOESTER PHOSPHOHYDROLASE [ALKALINE OPTIMUM] TYPE III-L

PHOSPHORIC MONOESTER PHOSPHOHYDROLASE [ALKALINE OPTIMUM] TYPE III-S

PHOSPHORIC MONOESTER PHOSPHOHYDROLASE [ALKALINE OPTIMUM] TYPE I-S

PHOSPHORIC MONOESTER PHOSPHOHYDROLASE [ALKALINE OPTIMUM] TYPE IV

PHOSPHORIC MONOESTER PHOSPHOHYDROLASE [ALKALINE OPTIMUM] TYPE VIII

PHOSPHORIC MONOESTER PHOSPHOHYDROLASE [ALKALINE OPTIMUM] TYPE VII-L

PHOSPHORIC MONOESTER PHOSPHOHYDROLASE [ALKALINE OPTIMUM] TYPE VII-S

PHOSPHORIC MONOESTER PHOSPHOHYDROLASE [ALKALINE OPTIMUM] TYPE VII-T

PHOSPHORIC MONOESTER PHOSPHOHYDROLASE [ALKALINE OPTIMUM] TYPE XVII

PHOSPHORIC MONOESTER PHOSPHOHYDROLASE [ALKALINE OPTIMUM] TYPE XXIV

PHOSPHORIC MONOESTER PHOSPHOHYDROLASE [ALKALINE OPTIMUM] TYPE XXXII

PHOSPHORIC MONOESTER PHOSPHOHYDROLASE [ALKALINE OPTIMUM] TYPE XXXIII

PHOSPHATASE, ALKALINE BOVINE

PHOSPHATASE, ALKALINE CALF INTESTINE

PHOSPHATASE, ALKALINE, E COLI

PHOSPHATASE, ALKALINE, HUMAN PLACENTA

PHOSPHATASE, ALKALINE TYPE III

PHOSPHATASE, ALKALINE TYPE III-L

PHOSPHATASE, ALKALINE TYPE III-S

PHOSPHATASE, ALKALINE TYPE I-S

PHOSPHATASE, ALKALINE TYPE IV

PHOSPHATASE, ALKALINE TYPE VIII

PHOSPHATASE, ALKALINE TYPE VII-L

PHOSPHATASE, ALKALINE TYPE VII-S

PHOSPHATASE, ALKALINE TYPE VII-T

PHOSPHATASE, ALKALINE TYPE XVII

PHOSPHATASE, ALKALINE TYPE XXIV

PHOSPHATASE, ALKALINE TYPE XXXII

PHOSPHATASE, ALKALINE TYPE XXXIII

PHOSPHATASE, ALKALINE

PHOSPHTASE ALKALINE

Alkaline phosphatase (catalytic concentration)

phosphatase alk. from calf intestinal mucosa

Phosphatase alkaline bovine intestinal mucosa

Phosphatase alkaline calf intestinal mucosa

Alkaline Phosphatase from E. coli

Phosphatase, Alkaline from bovine milk

Phosphatase alkaline, immobilized on Agarose from calf intestinal mucosa

Phosphatase, Alkaline from canine intestine

AKP

phosphomonesterase, alkaline, calf intestinal mucosa

ALKALINE PHOSPHATASE SOLUTION, FROM CALF INTESTINE

PHOSPHATASE, ALKALINE BACTERIAL (BAP)

PHOSPHATASE, ALKALINE FROM CALF INTESTINE (CIP)

5-Allyl-5-(2-hydroxypropyl)-1-phenyl-2,4,6(1H,3H,5H)-pyrimidinetrione