C6-NBD-CERAMIDE Property

Melting point:

85-88 °C

Density 

1.153±0.06 g/cm3(Predicted)

storage temp. 

−20°C

solubility 

Soluble in DMSO (35 mg/mL)

pka

13.54±0.20(Predicted)

form 

solid

color 

Orange

Stability:

Stable for 2 years as supplied. Solutions in DMSO may be stored at -20°C for up to 1 month.

Safety

WGK Germany 

3

Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Warning

Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P271Use only outdoors or in a well-ventilated area.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P302+P352IF ON SKIN: wash with plenty of soap and water.

P304+P340IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

P312Call a POISON CENTER or doctor/physician if you feel unwell.

P321Specific treatment (see … on this label).

P332+P313IF SKIN irritation occurs: Get medical advice/attention.

P337+P313IF eye irritation persists: Get medical advice/attention.

P403+P233Store in a well-ventilated place. Keep container tightly closed.

P405Store locked up.

P501Dispose of contents/container to..…

C6-NBD-CERAMIDE Chemical Properties,Usage,Production

Description

C6 NBD ceramide is a biologically active fluorescent analog of short chain, membrane-permeable ceramides. It is as effective as C6 ceramide in the inhibition of viral glycoprotein transport through the Golgi. C6 NBD ceramide has been used as a fluorescent substrate for the activity of UDP-glucose:ceramide glucosyltransferase and to demonstrate the translocation of glucocerebroside and sphingomyelin from the Golgi to the plasma membrane.

Chemical Properties

Rust or Dark Yellow Solid

Uses

A vital fluoresent stain for the Golgi apparatus.

Definition

ChEBI: N-{6-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]hexanoyl}sphingosine is an N-acylsphingosine that is sphingosine with the amino nitrogen converted into a 6-{[N-(7-nitrobenzo-2,1,3-oxadiazol-4-yl)amino]}hexananamido group. It has a role as a fluorescent probe.

References

Chazotte (2012),?Labeling Golgi with Fluorescent Ceramides;?Cold Spring Harb. Protoc. (8):pdb.prot070599 van Meer et al. (1987),?Sorting of sphingolipids in epithelial (Madin-Darby canine kidney) cells; J. Cell Biol.?105 1623 Don and Rosen (2008),?A fluorescent plate reader assay for ceramide kinase; Anal. Biochem.?375 265 Nikolova-Karakashian (2021),?Methods to Characterize Synthesis and Degradation of Sphingomyelin at the Plasma Membrane and Its Impact on Lipid Raft Dynamics; Methods Mol. Biol.?2187 113