Description Uses
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SEPHADEX G-50

Description Uses
Product Name
SEPHADEX G-50
CAS No.
9048-71-9
Chemical Name
SEPHADEX G-50
Synonyms
PDXTM G.F.;SEPHADEX G-50;Dextran gel G50;Sephadex G50, BR;SEPHADEX(R) G-50;SEPHADEX G-50 FINE;PDX GF 50 (50-150);PDX GF 50 (100-300);SEPHADEX G-50 COARSE;Sephadex G-50 60-100
CBNumber
CB3728066
Molecular Formula
NULL
Formula Weight
0
MOL File
Mol file
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SEPHADEX G-50 Property

form 
Solid
color 
White to off-white
CAS DataBase Reference
9048-71-9
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Safety

WGK Germany 
3
HS Code 
39140000
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Hazard and Precautionary Statements (GHS)

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
G5080
Product name
Sephadex? G-50
Purity
Fine
Packaging
50g
Price
$491
Updated
2024/03/01
Sigma-Aldrich
Product number
G5080
Product name
Sephadex? G-50
Purity
Fine
Packaging
10g
Price
$145
Updated
2024/03/01
Biosynth Carbosynth
Product number
YS165316
Product name
Sephadex G50
Packaging
50g
Price
$160
Updated
2021/12/16
Biosynth Carbosynth
Product number
YS165316
Product name
Sephadex G50
Packaging
100g
Price
$250
Updated
2021/12/16
Biosynth Carbosynth
Product number
YS165316
Product name
Sephadex G50
Packaging
10g
Price
$70
Updated
2021/12/16
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SEPHADEX G-50 Chemical Properties,Usage,Production

Description

Sephadex G-50 Superfine is a well established gel filtration medium for desalting and buffer exchange of biomolecules >30 000 molecular weight. The Superfine´s small bead size give higher efficiency.

  1. Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
  2. Suitable for purification of DNA from small molecules by gel filtration
  3. Small bead size for shorter diffusion distances
  4. Smallest bead size in the Sephadex G-50 range
Sephadex is a gel filtration medium prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex G-50 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-50 is available in 4 different particle sizes (Coarse, Medium, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency with shorter diffusion distances. Coarse and Medium are are preferred for large scale group separations where high flow rates and low operating pressures are required.

Uses

The media used for gel exclusion chromatography include dextran (Sephadex™), polyacrylamide (Bio-Gel P™) and dextran-polyacrylamide (Sephacryl™) and agarose (Sepharose™ and BioGel A™). Each is available with a variety of different ranges of pore size in the beads, permitting separation of macromolecules of different size.
A gel with a smaller range of pore sizes (and hence a smaller range over which it can separate macromolecules on the basis of their size) will give a higher resolution; a gel with a wider range will give lower resolution, but will permit fractionation of a larger range of sizes as an initial step when the approximate molecular mass of the enzyme is not known.
The particle size of the gel beads (the mesh size) also affects resolution; smaller beads permit higher resolution, but a lower flow rate through the column (and hence a slower separation).
The table shows the useful range for the most commonly used gel filtration media - the lower and upper molecular sizes (in kDa) over which they can be used to separate macromolecules. The upper limit is known as the exclusion limit of the gel - the size above which proteins will elute in the void volume of the column.

 
lower limit
upper limit
dextran gels
Sephadex G-50
1.5
30
Sephadex G-75
3
80
Sephadex G-100
4
150
Sephadex G-150
5
300
Sephadex G-200
5
600
polyacrylamide gels
Bio-Gel P-10
1.5
20
Bio-Gel P-30
2.5
40
Bio-Gel P-60
3
60
Bio-Gel P-100
5
100
Bio-Gel P-150
15
150
BioGel P-200
30
200
Bio-Gel P-300
60
400
dextran-polyacrylamide gels
Sephacryl S-200
5
250
Sephacryl S-300
10
1500
Sephacryl S-400
20
8000
agarose gels
Sepharose 6B
10
4000
Sepharose 4B
60
20,000
Sepharose 2B
70
40,000
Bio-Gel A-0.5
10
500
Bio-Gel A-1.5
10
1500
Bio-Gel A-5
10
5000
Bio-Gel A-15
40
15,000
Bio-Gel A-50
100
50,000

Uses

Sephadex? G-50 is a gel filtration medium used in affinity chromatography, protein chromatrography and gel filtration chromatography.
Fractionation Range (MW)
Globular Proteins: 1,500 - 30,000
Dextrans: 500 - 10,000

SEPHADEX G-50 Preparation Products And Raw materials

Raw materials

Preparation Products

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SEPHADEX G-50 Suppliers

China 133 Switzerland 1 United States 6 Global 140
Aladdin Scientific
Tel
+1-+1(833)-552-7181
Email
sales@aladdinsci.com
Country
United States
ProdList
57505
Advantage
58
Riedel-de Haen AG
Tel
--
Fax
--
Country
United States
ProdList
6773
Advantage
87
Advanced Synthesis Technologies
Tel
--
Fax
--
Email
sales@advancedsynthesis.com
Country
United States
ProdList
4775
Advantage
61
Amersham Biosciences, Inc.
Tel
--
Fax
--
Country
United States
ProdList
1270
Advantage
85
Pfaltz & Bauer, Inc.
Tel
--
Fax
--
Email
sales@pfaltzandbauer.com
Country
United States
ProdList
6828
Advantage
72
MP Biomedicals, Inc.
Tel
--
Fax
--
Country
United States
ProdList
6561
Advantage
81
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View Lastest Price from SEPHADEX G-50 manufacturers

Career Henan Chemical Co
Product
Sephadex G-50 9048-71-9
Price
US $1.00/kg
Min. Order
1kg
Purity
95%-99%
Supply Ability
1000kg
Release date
2018-12-25

9048-71-9, SEPHADEX G-50Related Search:

SEPHADEX G-75 SEPHADEX G-100 Dextran Sulfate Sodium Salt Iron-dextran SEPHADEX G-25 SEPHADEX G-15 SEPHADEX G-10 SEPHADEX G-200 Tris(2,4-pentanedionato)chroMiuM(III) COBALT(II) ACETYLACETONATE 1,1,3,3-TETRAMETHYLBUTYL ISOCYANIDE SALCOMINE DICHLORO(ETHYLENEDIAMINE)PLATINUM(II) Ferric acetylacetonate Aluminum acetylacetonate TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)EUROPIUM(III) N-BUTYLISOCYANIDE Cross-linked dextran gel CM C-50

  • Sephadex G50, BR
  • PDXTM G.F.
  • Sephadex<sup>(R)</sup> G-50 Fine
  • Sephadex G-50, Medium beads
  • PDX GF 50 (100-300)
  • PDX GF 50 (50-150)
  • SEPHADEX G-50
  • SEPHADEX G-50 COARSE
  • SEPHADEX G-50 FINE
  • Sephadex G-50, Fine beads
  • Cross-inked dextran gel G-50
  • Sephadex G-50 100-200
  • Sephadex G-50 200-400
  • Sephadex G-50 60-100
  • SEPHADEX G-50 MEDIUM
  • SEPHADEX G-50 SUPERFINE
  • SEPHADEX(R) G-50
  • sephadex(R) G-50 fine for chromato-graphy
  • sephadex(R) G-50 medium for chromato-graphy
  • sephadex(R) G-50 superfine for chromato-graphy
  • Cross-linked dextran gel G-50
  • SEPHADEX(R) G-50 MEDIUM FOR CHROMATO- GRAPHY, 50-150 UM*
  • SEPHADEX G-50, FOR GEL FILTRATION, 100-300MICRON
  • SEPHADEX G-50 MEDIUM DNA GRADE
  • SEPHADEX G-50, FOR GEL FILTRATION, 50-15 0MICRON
  • SEPHADEX(R) G-50 FINE FOR CHROMATO- GRAPHY, 20-80 UM*
  • SEPHADEX G-50, FOR GEL FILTRATION, 20-50MICRON
  • D(+)-GLUCOSE MOLECULAR BIOLOGY REAGENT
  • SEPHADEX(R) G-50 SUPERFINE FOR CHROMATO- GRAPHY, 20-50 UM*
  • SEPHADEX G-50, FOR GEL FILTRATION, 20-80MICRON
  • SEPHADEX G-50 FINE DNA GRADE
  • SEPHADEX G-50, SUPERFINE 20-50MICRON
  • SEPHADEX G-50, FINE 20-80MICRON
  • SEPHADEX G-50, MEDIUM 50-150MICRON
  • SEPHADEX G-50, COARSE 100-300MICRON
  • Dextran gel G50
  • Sephadex G 50 (9CI, ACI)
  • Sephadex G-50 (100-300um)
  • Dextran Gel G-50 60-100
  • 9048-71-9
  • DNA &amp; RNA Purification
  • Gel Filtration Media
  • Gel Filtration Chromatography
  • Sephadex
  • Resins
  • Resins and Media
  • Reagents
  • Protein Analysis
  • Protein Chromatography
  • Proteomics and Protein Expression
  • Molecular Biology
  • BioChemical
  • Analytical Chromatography Product Catalog
  • AMEKO
  • Gel Filtration Media
  • Sephadex G-50
  • Gel Filtration Chromatography
  • Gel Filtration MediaGel Filtration Media