Description History
ChemicalBook > CAS DataBase List > SEPHADEX G-100

SEPHADEX G-100

Description History
Product Name
SEPHADEX G-100
CAS No.
9050-94-6
Chemical Name
SEPHADEX G-100
Synonyms
SEPHADEX G-100;SEPHADEX(R) G-100;Sephadex G-100 60-100;Sephadex G-100 200-400;Sephadex G-100 100-200;Dextran Gel G-10 60-100;SEPHADEX G-100 SUPERFINE;SEPHADEX G-100 DNA GRADE;SEPHADEX G-100 USP/EP/BP;Gel filtration medium G-100
CBNumber
CB7267721
Molecular Formula
NULL
Formula Weight
0
MOL File
Mol file
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SEPHADEX G-100 Property

Flash point:
38 °C
storage temp. 
2-8°C
form 
beads
color 
White to off-white
PH
2 - 10
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Safety

Risk Statements 
10
Safety Statements 
22-16
WGK Germany 
3
HS Code 
39139000
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Hazard and Precautionary Statements (GHS)

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
GE17-0061-01
Product name
Sephadex® G-100 Superfine
Purity
Cytiva, 17-0061-01, pack of 100?g
Packaging
1pkg
Price
$1010
Updated
2024/03/01
Sigma-Aldrich
Product number
GE17-0060-01
Product name
Sephadex® G-100
Purity
Cytiva, 17-0060-01, pack of 100?g
Packaging
1pkg
Price
$743
Updated
2024/03/01
Sigma-Aldrich
Product number
G100120
Product name
Sephadex? G-100
Purity
Medium
Packaging
10g
Price
$321
Updated
2024/03/01
Sigma-Aldrich
Product number
G100120
Product name
Sephadex? G-100
Purity
Medium
Packaging
50g
Price
$1230
Updated
2024/03/01
Sigma-Aldrich
Product number
S6147
Product name
Sephadex? G-100
Purity
BioReagent, for molecular biology, DNA grade, medium
Packaging
25g
Price
$336
Updated
2024/03/01
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SEPHADEX G-100 Chemical Properties,Usage,Production

Description

Sephadex® G-100 is a gel filtration medium used in protein chromatography, affinity chromatography and gel filtration chromatography.
Sephadex G-100 is useful for desalting and buffer exchange of very large biomolecules.

  1. Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
  2. Classic gel filtration medium.
Sephadex G-10 is a well established gel filtration medium for desalting and buffer exchange of peptides and small biomolecules >700 molecular weight. Smallest exclusion limit in the Sephadex range.
  1. Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
  2. Suitable for buffer exchange and cleanup of biological samples, for example peptides, small proteins, and oligosaccharides larger than 700 molecular weight
  3. Efficient removal of contaminants such as salts, dyes, and radioactive labels
  4. Lowest exclusion limit (700 molecular weight) in the Sephadex G-range
Sephadex is a gel filtration medium prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex G-10 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-50 is available in 4 different particle sizes (Coarse, Medium, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency fractionation with shorter diffusion distances. Coarse and Medium are are preferred for large scale group separations where high flow rates and low operating pressures are required.
Sephadex is a registered trademark of GE Healthcare

History

The introduction of Sephadex in 1959 heralded a new era in separation science. The concept of gel permeation chromatography (GPC) developed slowly and the key events leading to the discovery of Sephadex may be traced to Ingelman ?s early studies on the cross-linking of dextran, the subsequent patent by Flodin and Ingelman on the preparation of cross-linked gels and the exploitation of their gel filtration properties by Flodin and Porath.
Perhaps the most critical event was the synthesis of these gels in bead form with considerably improved flow rates, an innovation that led to the immediate acceptance of these gels in separation technology. Several types of Sephadex are currently available, each with a characteristic fractionation range. The most porous gel, Sephadex G-200, will fractionate proteins in the Mw range 4000–800000, whereas the upper limit for Sephadex G-25 is 5000. Although Sephadex gels are widely used for fractionation and purification of biopolymers, desalting constitutes one of the most important applications. In this context, desalting also refers to buffer exchange operations and the removal of low molecular weight impurities other than salts. Sephadex G-25 is now used for desalting or buffer exchange in many large scale operations. Insulin producers use Sephadex G-50 to remove proinsulin and protease impurities in the final stages of purification of porcine or bovine insulins.
The structure of Sephadex G-25 has recently been investigated by Holmberg (285) and the following novel structural features have come to light.

  1. Only 4% of the glucose units are cross-linked.
  2. Eight different structural elements were identified, including several glyceryl ethers of glucose and cyclic structures containing 1,4-dioxane rings. Two of these fragments are represented below 3 and 4.
  3. 80% of the beads consists of dextran.

Uses

Sepharose? 2B has been used for the preparation of virosomes and platelets.

Uses

Fractionation Range (MW)
Globular Proteins: 4,000 - 100,000
Dextrans: 1,000 - 100,000

Uses

Sephadex?G-100 is a gel filtration medium used in protein chromatography, affinity chromatography and gel filtration chromatography
It has been used:

  • in gel filtration column for α-amylase purification
  • in gel filtration column for the purification of polysaccharidic fraction extracted from the skin of the ray Raja radula
  • to remove excess protamine by size exclusion from cross-linked iron oxide (CLIO)-protamine-linked (B) peptides

General Description

Sepharose 2B is a well-proven cross-linked agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling.

SEPHADEX G-100 Preparation Products And Raw materials

Raw materials

Preparation Products

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SEPHADEX G-100 Suppliers

China 70 Switzerland 1 United States 5 Global 76
Beijing Baileisen Biotechnology Co., Ltd.
Tel
13581502185
Email
joe0415zhaxi@163.com
Country
China
ProdList
141
Advantage
58
future industrial shanghai co., ltd
Tel
400-0066400 13621662912
Fax
021-55660885
Email
sales@jonln.com
Country
China
ProdList
1995
Advantage
65
Adamas Reagent, Ltd.
Tel
400-6009262 16621234537
Fax
021-64823266
Email
chenyj@titansci.com
Country
China
ProdList
14103
Advantage
59
Tianjin heowns Biochemical Technology Co., Ltd.
Tel
400 638 7771
Email
sales@heowns.com
Country
China
ProdList
14435
Advantage
57
Wuhan Fortuna Chemical Co., Ltd
Tel
027-59207852 13308628970
Fax
QQ3130921841
Email
buy@fortunachem.com
Country
China
ProdList
2866
Advantage
58
ShangHai YuanYe Biotechnology Co., Ltd.
Tel
021-61312847 13636370518
Fax
021-55068248
Email
shyysw007@163.com
Country
China
ProdList
4940
Advantage
60
Shanghai Ruji Biology Technology Co., Ltd.
Tel
+86-21-65211385-8001 36031160
Fax
+86-21-65211385-8004
Email
sales@shruji.com
Country
China
ProdList
3224
Advantage
55
Nanjing Dulai Biotechnology Co., Ltd.
Tel
025-84699383-8003 18013301590
Fax
025-84699383-8003
Email
njduly@126.com
Country
China
ProdList
971
Advantage
55
Shanghai BeiZhuo Biotech Co., Ltd.
Tel
021-61119791,13386096464
Fax
021-50190009
Email
bzswkf@foxmail.com
Country
China
ProdList
3921
Advantage
50
Shanghai JONLN Reagent Co., Ltd.
Tel
400-0066400 13621662912
Fax
021-55660885
Email
422131432@qq.com
Country
China
ProdList
9978
Advantage
55
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View Lastest Price from SEPHADEX G-100 manufacturers

Career Henan Chemical Co
Product
Sepharose 2B 9050-94-6
Price
US $1.00/kg
Min. Order
1kg
Purity
95%-99%
Supply Ability
1000kg
Release date
2018-12-25

9050-94-6, SEPHADEX G-100Related Search:

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  • Cross-inked dextran gel G-100
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  • Cross-inked dextran gel G-75
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  • Sephadex G-100 60-100
  • sephadex(R) G-100 for chromatography
  • SEPHADEX G-100 SUPERFINE
  • SEPHADEX G-100, FOR GEL FILTRATION, 20-50MICRON
  • SEPHADEX(R) G-100 FOR CHROMATOGRAPHY, 40-120 UM*
  • SEPHADEX G-100 DNA GRADE
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  • SEPHADEX G-100, 40-120MICRON DRY BEAD DIAMETER: 40-120 μG
  • SEPHADEX G-100, SUPER FINE 20-50MICRON DRY BEAD DIAMETER: 20-50 μG
  • Sephadex G-100, Medium beads
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  • SEPHADEX G-100 USP/EP/BP
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  • Dextran Gel G-10 60-100
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