CHAPS
- Product Name
- CHAPS
- CAS No.
- 75621-03-3
- Chemical Name
- CHAPS
- Synonyms
- CHAPS;TREND;3-[(3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO]-1-PROPANESULFONATE;N,N-Dimethyl-N-(3-sulfonatopropyl)-3-[(3α,7α,12α-trihydroxy-24-oxo-5β-cholan-24-yl)amino]propylaminium;N,N-Dimethyl-N-(3-sulfonatopropyl)-3-[[3α,7α,12α-trihydroxy-24-oxo-5β-cholan-24-yl]amino]-1-propaneaminium;1-Propanaminium, N,N-dimethyl-N-(3-sulfopropyl)-3-[[(3a,5b,7a,12a)-3,7,12-trihydroxy-24-oxocholan-24-yl]amino]-, inner salt;CHAPS, 98+%;CHAPS buffer;CHAPS 100 mM;CHAPS, >=99%
- CBNumber
- CB4165631
- Molecular Formula
- C32H58N2O7S
- Formula Weight
- 614.88
- MOL File
- 75621-03-3.mol
CHAPS Property
- Melting point:
- 156-158°C (dec.)
- Density
- 1.01 g/mL at 20 °C
- refractive index
- 1.6900 (estimate)
- storage temp.
- -20°C
- solubility
- H2O: 50 mg/mL at 20 °C, clear, colorless
- form
- Crystalline Powder
- color
- White
- Odor
- Odorless
- PH Range
- 6
- Water Solubility
- Soluble in water (10 mg/ml). Soluble in DMF and hot methanol.
- Sensitive
- Hygroscopic
- Merck
- 2044
- Stability:
- Moisture Sensitive: Hydroscopic
- InChIKey
- UMCMPZBLKLEWAF-RFCNGIAKSA-N
Safety
- Hazard Codes
- Xi,T
- Risk Statements
- 36/37/38-61
- Safety Statements
- 26-36-45-53
- WGK Germany
- 3
- F
- 3-10
- HS Code
- 29239000
Hazard and Precautionary Statements (GHS)
- Symbol(GHS)
-
- Signal word
- Danger
- Precautionary statements
-
P201Obtain special instructions before use.
P202Do not handle until all safety precautions have been read and understood.
P280Wear protective gloves/protective clothing/eye protection/face protection.
P308+P313IF exposed or concerned: Get medical advice/attention.
P405Store locked up.
P501Dispose of contents/container to..…
N-Bromosuccinimide Price
- Product number
- 220201
- Product name
- CHAPS,MolecularBiologyGrade-CAS75621-03-3-Calbiochem
- Purity
- CAS75621-03-3isazwitterionicdetergentthatcombinesfeaturesofbilesalts
- Packaging
- 1g
- Price
- $81
- Updated
- 2024/03/01
- Product number
- 220201
- Product name
- CHAPS,MolecularBiologyGrade-CAS75621-03-3-Calbiochem
- Purity
- CAS75621-03-3isazwitterionicdetergentthatcombinesfeaturesofbilesalts
- Packaging
- 5G
- Price
- $239
- Updated
- 2024/03/01
- Product number
- 220201
- Product name
- CHAPS,MolecularBiologyGrade-CAS75621-03-3-Calbiochem
- Purity
- CAS75621-03-3isazwitterionicdetergentthatcombinesfeaturesofbilesalts
- Packaging
- 10g
- Price
- $398
- Updated
- 2024/03/01
- Product number
- 220201
- Product name
- CHAPS,MolecularBiologyGrade-CAS75621-03-3-Calbiochem
- Purity
- CAS75621-03-3isazwitterionicdetergentthatcombinesfeaturesofbilesalts
- Packaging
- 25g
- Price
- $786
- Updated
- 2024/03/01
- Product number
- 220201
- Product name
- CHAPS,MolecularBiologyGrade-CAS75621-03-3-Calbiochem
- Purity
- CAS75621-03-3isazwitterionicdetergentthatcombinesfeaturesofbilesalts
- Packaging
- 250g
- Price
- $2600
- Updated
- 2024/03/01
CHAPS Chemical Properties,Usage,Production
Chemical Properties
White crystalline powder. CHAPS may be synthesized from cholic acid and is zwitterionic due to its quaternary ammonium and sulfonate groups; it is structurally similar to certain bile acids, such as taurodeoxycholic acid and taurochenodeoxycholic acid. It is sparingly soluble or insoluble in aqueous solution due to their native hydrophobicity.
Uses
CHAPS is a zwitterionic nondenaturing detergent for solubilizing membrane proteins. It is used in the laboratory to dissolve biological macromolecules such as proteins. It acts as a non-denaturing solvent used in purification of membrane proteins. It is also useful in conjunction with nonionic detergents like Triton X-100. Further, it is used as a non-denaturing zwitterionic detergent for membrane biochemistry.
Application
CHAPS is often used as a detergent in the solubilization and purification of membrane proteins for several advantageous reasons. CHAPS detergent is non-denaturing to membrane proteins, can solubilize proteins, disaggregate protein-protein interactions. CHAPS is also useful in ion exchange chromatography and isoelectric focusing as it is zwitterionic and does not exhibit a net charge between pH 2 to 12. It is suitable for isolation of membrane proteins with the following features such as sufficient protein solubilization capability, no denaturing or inactivation of proteins, no interference with protein activities, no precipitation at 4°C, appropriate critical micelle concentrations (CMC) and micelle size, no absorption in the UV region, no toxicity and availability of detergent detection methods.
General Description
CHAPS is a stabilizing agent that can be used to prevent aggregation, precipitation or proteolysis of proteins in solution.
Biological Activity
CHAPS is a zwitterionic nondenaturing detergent for solubilizing membrane proteins. Combines the properties of both the sulfobetaine-type and bile-acid detergents.
storage
Room temperature
Purification Methods
Triturate this zwitterionic detergent (~50g) with MeOH (200ml), then triturate it with Me2CO (500mL), collect it by vacuum filtration and dry it thoroughly at ~20o. Recrystallise it at 0o from absolute MeOH followed by drying in vacuo to constant weight. It should give one spot with RF 0.32 by TLC on silica gel G in 95% MeOH/5% NH4OH and visualized with iodine vapour; the tertiary amine precursor has RF 0.40. It has low UV absorption (1% in H2O: A280nm is 0.029 and A260nm is 0.035), and the CMC (critical micellar concentration) is 8mM. It is soluble in H2O, MeOH, Me2SO but insoluble in Me2CO, MeCN, hexane, toluene and xylene. [Hjelmeland et al. Anal Biochem 130 72 1983, Matuo et al. Methods Enzymol 198 155 1991.] CHAPSO (3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate betaine) [82473-24-3] M 630.89, m 184-196o, [ ] D +19o (c 2, H2O), pKEst (1) ~<2 (acidic), pKEst(2) ~9 (basic). Dissolve this zwitterionic detergent (~ 60g) in 40% aqueous MeOH and stir it with the mixed-bed ion-exchange resin RG-501 x 8 (100g, Bio-Rad) until the pH of the supernatant is ~ 7. Filter off the resin and evaporate the filtrate to dryness under reduced pressure. Check for homogeneity by TLC on silica gel G in 95% MeOH/5% NH4OH and visualize the spot with iodine vapour (see CHAPS above). [Hjelmeland et al. Anal Biochem 130 72 1983, Matuo et al. Methods Enzymol 198 155 1991.]
CHAPS Preparation Products And Raw materials
Raw materials
Preparation Products
CHAPS Suppliers
- Tel
- 15866604075 15866604075
- 15195457346m@sina.cn
- Country
- China
- ProdList
- 513
- Advantage
- 58
- Tel
- +86-025-81682922 +86-13515111588
- Fax
- 025-85860962
- sales@searchbio.com.cn
- Country
- China
- ProdList
- 135
- Advantage
- 68
- Tel
- 0512-62968256 18550502935
- Fax
- 0512-62968256
- export@laingbiotec.com
- Country
- China
- ProdList
- 1037
- Advantage
- 58
- Tel
- 18051857768
- sales@hi-pure.cn
- Country
- China
- ProdList
- 35
- Advantage
- 58
- Tel
- 13921770081
- SVP01@magicbiotech.cn
- Country
- China
- ProdList
- 384
- Advantage
- 58
- Tel
- 0731-85561020 15386497078
- yunbang@yunbangapi.com
- Country
- China
- ProdList
- 4200
- Advantage
- 58
- Tel
- 18221419461; 18221419461
- sales@yikefan.com
- Country
- China
- ProdList
- 612
- Advantage
- 58
- Tel
- 18008681304
- 898948847@qq.com
- Country
- China
- ProdList
- 84
- Advantage
- 58
- Tel
- 0731-85526065 13308475853
- ivy@hnhbsj.com
- Country
- China
- ProdList
- 4550
- Advantage
- 62
- Tel
- 010-82848833 400-666-7788
- Fax
- 86-10-82849933
- jkinfo@jkchemical.com
- Country
- China
- ProdList
- 94838
- Advantage
- 76
View Lastest Price from CHAPS manufacturers
- Product
- 3-((3-Cholamidopropyl)dimethylammonium)-1-propanesulfonate 75621-03-3
- Price
- US $0.00/kg
- Min. Order
- 1kg
- Purity
- 0.99
- Supply Ability
- 20 tons
- Release date
- 2023-11-21
- Product
- CHAPS 75621-03-3
- Price
- US $0.00/KG
- Min. Order
- 1KG
- Purity
- 99%
- Supply Ability
- 3500kg/month
- Release date
- 2021-09-10
- Product
- 3-((3-Cholamidopropyl)dimethylammonium)-1-propanesulfonate 75621-03-3
- Price
- US $30.00-10.00/KG
- Min. Order
- 50KG
- Purity
- 99%
- Supply Ability
- 500000kg
- Release date
- 2022-09-20