description Uses
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PIPES

description Uses
Product Name
PIPES
CAS No.
5625-37-6
Chemical Name
PIPES
Synonyms
PIPES FREE ACID;1,4-Piperazinebis(ethanesulphonic acid);PIPES;PIPES,98%;PIPES0215;Pipes AR 98%;PIPES【Dotite】;Pipes(Buffer);PIPES solution;MOLYBDENE STAND
CBNumber
CB4435134
Molecular Formula
C8H18N2O6S2
Formula Weight
302.37
MOL File
5625-37-6.mol
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PIPES Property

Melting point:
>300 °C (lit.)
Density 
1.4983 (rough estimate)
refractive index 
1.6300 (estimate)
storage temp. 
Sealed in dry,Room Temperature
solubility 
0.1 M NaOH: 0.25 g/mL, clear, colorless
form 
Micro-Crystalline Powder
color 
White
pka
6.8(at 25℃)
PH Range
6.1 - 7.5
Water Solubility 
Soluble in water.
λmax
λ: 260 nm Amax: 0.055
λ: 280 nm Amax: 0.040
Merck 
14,7479
BRN 
817713
InChIKey
XWGRYGKFRRLUQQ-UHFFFAOYSA-N
LogP
-2.7 at 20℃
CAS DataBase Reference
5625-37-6(CAS DataBase Reference)
EPA Substance Registry System
1,4-Piperazinediethanesulfonic acid (5625-37-6)
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Safety

Hazard Codes 
Xi
Risk Statements 
36/37/38
Safety Statements 
22-24/25-37/39-26
WGK Germany 
3
TSCA 
Yes
HS Code 
29335995
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Warning
Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P271Use only outdoors or in a well-ventilated area.

P280Wear protective gloves/protective clothing/eye protection/face protection.

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
80635
Product name
PIPES
Purity
BioXtra, for molecular biology, ≥99.5% (T)
Packaging
50g
Price
$281
Updated
2024/03/01
Sigma-Aldrich
Product number
80635
Product name
PIPES
Purity
BioXtra, for molecular biology, ≥99.5% (T)
Packaging
250g
Price
$1110
Updated
2024/03/01
Sigma-Aldrich
Product number
RES0703P-A7
Product name
PIPES
Purity
PharmaGrade, Manufactured under appropriate controls for use as a raw material in pharma or biopharmaceutical production, suitable for cell culture
Packaging
1pkg
Price
$188
Updated
2022/05/15
Alfa Aesar
Product number
A16090
Product name
PIPES, 98%
Packaging
25g
Price
$37.5
Updated
2024/03/01
Alfa Aesar
Product number
A16090
Product name
PIPES, 98%
Packaging
100g
Price
$103
Updated
2024/03/01
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PIPES Chemical Properties,Usage,Production

description

PIPES free acid, also known as 1,4-piperazinediethanesulfonic acid, is a Good's buffer notable for having a pKa value similar to ordinary physiological pH. As such, PIPES free acid is frequently used as a buffer in biochemical research.
PIPES is a zwitterionic, piperazinic buffer that is useful for a pH range of 6.1 – 7.5. PIPES lacks the ability to form a significant complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions. PIPES has wide variety of applications and is commonly used in cell culture media, in protein crystallization, as a running buffer in gel electrophoresis, and as an eluent in isoelectric focusing and chromatography. This buffer is capable of forming radicals and is therefore not suitable for redox reactions. It is suitable for use in the bicinchoninic acid (BCA) assay. Solubility of PIPES increases when the free acid is converted to the sodium salt.
Buffers are made by adding base solution to PIPES free acid and titrating to the desired pH.

Uses

Piperazine-N, N'-bis- (2-ethanesulphonic acid) ultra -pure >99.5%) is an important chemical intermedia in all kinds of chemical fields. 1,4-Piperazinediethanesulfonic acid, piperazine-N, N’-bis- (2-ethanesulfonic acid) (PIPES) is also one of the zwitterion buffers and the most appropriate buffer for the biological experiments using an intracellular solution since the Pk2 of Pipes is 6.8 at 20 degrees Celsius.

Description

PIPES is a member of the ethanesulfonic acid buffer series, first introduced by Good et al., developed to meet certain criteria: midrange pKa, maximum water solubility and minimum solubility in all other solvents, minimal salt effects, minimal change in pKa with temperature, chemically and enzymatically stable, minimal absorption in visible or UV spectral range and easily synthesized. Since its pKa at 37 °C is near physiological pH, PIPES has applications in cell culture work.

Chemical Properties

White/clear crystalline powder

Uses

A buffering agent with a pKa near physiological pH.PIPES [piperazine-N,N′-bis(2-ethanesulfonic acid)] is frequently used as a buffering agent in biochemistry. It is an ethanesulfonic acid buffer developed by Good et al. in the 1960s. PIPES has a pKa near the physiological pH which makes it useful in cell culture work. It has been documented to minimize lipid loss when buffering glutaraldehyde histology in plant and animal tissues.
Additional forms available: PIPES, Sesquisodium Salt ; PIPES dipotassium salt; PIPES disodium salt; PIPES, Sodium Salt. Buffers can be prepared by adding a solution of base to PIPES free acid, titrating to the appropriate pH, or by mixing equimolar solutions of the monosodium salt and the disodium salt, titrating to the appropriate pH.

Uses

Protocols have been reported on the use of PIPES for separation of glyoxylated RNA in agarose gels, nuclease S1 mapping of RNA, and in ribonuclease protection assay protocols. PIPES has been used as a buffer in glutaraldehyde fixation of tissue samples.,
PIPES has been utilized in protein crystallization., The use of PIPES in the reconstitution of dissociated tubulin
α and β subunits after their resolution on immunoadsorbent gels has been described. PIPES has been recommended for use in buffers for the in vitro study of caspases 3, 6, 7, and 8.
A published study demonstrated the usefulness of PIPES as a non-metal ion complexing buffer in such applications as protein assays. PIPES has been used in cell culture for such applications as the engineering of a thermostable mutant membrane protein in Escherichia coli.

Definition

ChEBI: 2,2'-piperazine-1,4-diylbisethanesulfonic acid is a Good's buffer substance, pKa = 6.8 at 20 ℃. It is a conjugate acid of a 2,2'-piperazine-1,4-diylbisethanesulfonate.

Application

Glutaraldehyde fixation of plant and animal tissue samples can cause loss of lipid, leading to apparent morphological changes. Lipid loss and artifacts were minimized when PIPES was used to buffer the glutaraldehyde fixative.
Alkaline phosphatase activity is lost selectively from certain rat hepatocyte organelles when fixed for ultracytochemistry with cacodylate-buffered glutaraldehyde. When PIPES was used as buffer, retention of activity was 60% greater.
Fixation of fungal zoospores for fluorescence microscopy and electron microscopy was optimal with a combination of glutaraldehyde and formaldehyde in PIPES buffer.

General Description

PIPES is a member of the ethanesulfonic acid buffer series, first introduced by Good et al., developed to meet certain criteria: midrange pKa, maximum water solubility and minimum solubility in all other solvents, minimal salt effects, minimal change in pKa with temperature, chemically and enzymatically stable, minimal absorption in visible or UV spectral range and easily synthesized. Since its pKa at 37 °C is near physiological pH, PIPES has applications in cell culture work.

Flammability and Explosibility

Not classified

Purification Methods

Purify PIPES from boiling water (maximum solubility is about 1g/L) or as described for ADA [N-(2-acetamido)iminodiacetic acid above]. [Good et al. Biochemistry 5 469 1966, Beilstein 23/12 V 380.]

PIPES Preparation Products And Raw materials

Raw materials

Preparation Products

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View Lastest Price from PIPES manufacturers

WUHAN FORTUNA CHEMICAL CO., LTD
Product
PIPES 5625-37-6
Price
US $0.00/KG
Min. Order
1KG
Purity
99%
Supply Ability
3500kg/month
Release date
2021-09-09
Hebei Weibang Biotechnology Co., Ltd
Product
PIPES 5625-37-6
Price
US $30.00-10.00/KG
Min. Order
50KG
Purity
99%
Supply Ability
500000kg
Release date
2022-09-21
Hebei Chuanghai Biotechnology Co,.LTD
Product
PIPES 5625-37-6
Price
US $10.00/kg
Min. Order
1kg
Purity
99%
Supply Ability
10000kg
Release date
2024-08-23

5625-37-6, PIPESRelated Search:

Piperazine Sodium diphenylamine-4-sulfonate Sodium xylenesulfonate Ethanesulfonic acid Dihydroergotoxine mesylate Ethyl 2-(Chlorosulfonyl)acetate Indigo Carmine Folic acid BUPIRIMATE Ascoric Acid POLYVINYL CHLORIDE, AMINATED Disodium piperazine-1,4-diethanesulphonate PIPES DIPOTASSIUM,PIPES-K2,PIPES DIPOTASSIUM SALT,PIPES 2K MOPS PIPES sesquisodium salt Polyperfluoro ethylene-propylene pipes, heat-shrinkable Sodium hydrogen piperazine-1,4-diethanesulphonate Sodium bis(trimethylsilyl)amide

  • PIPES,98%
  • PIPES Piperazine-1,4-bisethanesulfonic acid
  • PIPES Vetec(TM) reagent grade, >=99%
  • ABTS DIAMMONIUM SALT ULTRA PURE GRADE
  • MOLYBDENE STAND
  • MOLYBDENUM STAND
  • PIPES BIOXTRA, FOR MOLECULAR BIOLOGY
  • PIPES, BIOPERFORMANCE CERTIFIED&
  • LABOTEST-BB LT00138111
  • 1,4-PIPERAZINEBIS(ETHANESULFONIC ACID)
  • 1,4-PIPERAZINEDIETHANESULFONIC ACID
  • PIPERAZINE-1,4-BIS(2-ETHANESULFONIC ACID)
  • PIPERAZINE-NN'-BIS-2-ETHANESULPHONIC ACID
  • PIPERAZINE-N,N'-BIS(2-ETHANESULFONIC ACID)
  • PIPES
  • TIMTEC-BB SBB008973
  • 2,2-(piperazine-1,4-diyl)bis(ethanesulphonic) acid
  • PIPES Piperazine-N,N-bis(2-ethanesulfonic acid)
  • PIPES, Free Acid, MB Grade (1.12030)
  • PIPES, Free Acid, ULTROL Grade
  • PIPES, Molecular Biology Grade Piperazine-N,N-bis(2-ethanesulfonic acid), Molecular Biology Grade
  • Piperazine-1,4-bis(ethanesulfonic acid)
  • PIPERAZINE-1 4-BIS(2-ETHANESULFONIC ACI&
  • PIPES FREE ACID SIGMAULTRA
  • PIPES FREE ACID, BIOTECHNOLOGY PERFORMAN CE CERTIFIE
  • PIPES FREE ACID
  • PIPERAZINE-1,4-BIS(2-ETHANESULFONIC ACID ), F. MOL.BIOLOGY
  • SODIUM BIS(TRIMETHYLSILYL)AMIDE SOLUTION, WACKER QUALITY
  • Pipes(Buffer)-(Piperazine-N,N-Bis-2-EtheneSulfonicAcid)
  • 1,4-Piperazinebis(ethanesulphonic acid)
  • 1;4-PIPERAZINEDIETHANESULFONIC ACID;PIPERAZINE-N;N'-BIS(2-ETHANESULFONIC ACID);2;2'-(PIPERAZINE-1;4-DIYL)DIETHANESULFONIC ACID
  • 2'-(Piperazine-1
  • 4-diyl)diethanesulfonic acid
  • 4-Piperazinediethanesulfonic acid
  • N'-bis(2-ethanesulfonic acid)
  • PIPES0215
  • Piperazine -N'- bis (2-ethanesulfonic acid)
  • PIPERAZINE-N,N'-BIS(2-ETHANESULFONIC ACID)PIPERAZINE-N,N'-BIS(2-ETHANESULFONIC ACID)PIPERAZINE-N,N'-BIS(2-ETHANESULFONIC ACID)PIPERAZINE-N,N'-BIS(2-ETHANESULFONIC ACID)
  • 1,4-Piperazinediethanesulfonic acid(PIPES), 99.5%
  • PIPES solution
  • 2-[4-(2-sulfoethyl)-1-piperazinyl]ethanesulfonic acid
  • 1,4-Piperazinediethanesulfonic acid(PIPES)
  • Piperazine-1,4-diethyl acid
  • PIPES (Piperazin-1,4-bis(2-ethansulfonic acid)
  • Piperazine-N,N'-bis-2-ethanesulphonic acid ,99%
  • PIPERAZINE-N,N'-BIS(2-ETHANESULFONIC ACID) ANALYTICAL GRADE
  • PIPERAZINE-N,N'-BIS(2-ETHANE SULFONIC ACID)MOLECULAR BIOLOGY GRADE
  • PIPES,1,4-Piperazinediethanesulfonic acid, Piperazine-1,4-bis(2-ethanesulfonic acid), Piperazine-N,N′-bis(2-ethanesulfonic acid)
  • PIPES free acid, molecular biology grade
  • 1,4-Piperazinedietha
  • PIPES, Free Acid, ULTROL
  • PIPES (PIPERAZINE-1,4-BIS(2-ETHANESULFONIC ACID))
  • PIPES ULTRAPURE
  • PIPERAZINE-N,N-BIS(2-ETHANESULFONICACID)(PIPES)
  • Piperazine-N,N'-bis-(2-ethanesulphonic acid) ultrapure >99.5%
  • 1,4-Piperazinediethanesulfonic acid, Piperazine-1,4-bis(2-ethanesulfonic acid), Piperazine-N,Nμ-bis(2-ethanesulfonic acid)
  • 1,4-Piperazinebis(ethansulfonic acid)
  • 2,2'-(Piperazine-1,4-diyl)bis(ethanesulfonic) acid