HEPES

ChemicalBook > CAS DataBase List > HEPES

Product Name: HEPES
CAS No.: 7365-45-9
Chemical Name: HEPES
Synonyms: HEPES BUFFER;4-(2-HYDROXYETHYL)-1-PIPERAZINEETHANESULFONIC ACID;hepes solution;HYDROXYETHYLPIPERAZINE ETHANE SULFONIC ACID;Biological Buffer;HEPES buffer Solution;4-(2-HYDROXYETHYL)PIPERAZINE-1-ETHANESULFONIC ACID;4-(2-hydroxyethyl)piperazin-1-ylethanesulphonic acid;2-[4-(2-HYDROXYETHYL)-1-PIPERAZINYL]ETHANESULFONIC ACID;EPES
CBNumber: CB5408557
Molecular Formula: C8H18N2O4S
Formula Weight: 238.3
MOL File: 7365-45-9.mol

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HEPES Property

Melting point:: 234-238 °C
Boiling point:: 408℃[at 101 325 Pa]
Density: 1.07 g/mL at 20 °C
vapor pressure: 0Pa at 25℃
refractive index: n20/D 1.339
storage temp.: 2-8°C
solubility: H₂O: 1 M at 20 °C, clear, colorless
form: powder
color: White
PH Range: 7.0 - 7.6
PH: 5.0-6.5 (1Maqueous solution)
pka: 7.5(at 25℃)
Water Solubility: Soluble
λmax: λ: 260 nm Amax: 0.06
λ: 280 nm Amax: 0.04
Merck: 14,4654
BRN: 883043
Stability:: Stable. Combustible. Incompatible with strong oxidizing agents. Protect from moisture.
InChIKey: JKMHFZQWWAIEOD-UHFFFAOYSA-N
LogP: -3.85 at 20℃
CAS DataBase Reference: 7365-45-9(CAS DataBase Reference)
EPA Substance Registry System: 1-Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)- (7365-45-9)

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Safety

Hazard Codes: Xi
Risk Statements: 36/37/38
Safety Statements: 24/25-22-36-26
WGK Germany: 1
RTECS: TL6809000
TSCA: Yes
HS Code: 29335995

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Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Warning

Hazard statements

H302Harmful if swallowed

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P271Use only outdoors or in a well-ventilated area.

P280Wear protective gloves/protective clothing/eye protection/face protection.

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N-Bromosuccinimide Price

Sigma-Aldrich

Product number: PHG0001
Product name: HEPES
Packaging: 1KIT
Price: $978
Updated: 2024/03/01

Sigma-Aldrich

Product number: H4034
Product name: HEPES
Purity: BioPerformance Certified, ≥99.5% (titration), suitable for cell culture
Packaging: 25KG
Price: $6140
Updated: 2024/03/01

Sigma-Aldrich

Product number: H3375
Product name: HEPES
Purity: ≥99.5% (titration)
Packaging: 25KG
Price: $5870
Updated: 2024/03/01

Sigma-Aldrich

Product number: H3375
Product name: HEPES
Purity: ≥99.5% (titration)
Packaging: 50KG
Price: $11060
Updated: 2024/03/01

Sigma-Aldrich

Product number: 83264
Product name: HEPES buffer solution
Purity: 1?M in H2O
Packaging: 100ML
Price: $67.1
Updated: 2024/03/01

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HEPES Chemical Properties,Usage,Production

Description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). It is typically used in cell culture at concentration between 5mM to 30 mM. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Chemical Properties

White crystalline powder

Uses

Non-toxic to cell. It is used as a hydrogen ion buffer, which can control the constant pH range for long term. The concentration is 10-50mmol/L. Generally, 20mmol/LHEPES in nutrient solution can get buffer ability.

Uses

HEPES is common buffer for biological sciences, particularly used in cell culture to maintain physiological pH. It acts a buffering component, which is used in the preparation of buffers. It is described as one of the best all-purpose buffers available for use in biological research.
HEPES has been used as a component of:
Hank′s balanced salt solution, Dulbecco′s modified eagle′s medium and no-glucose DMEM, which are used for the preparation of tissue slices.
Buffers used in localized conductance measurements on the basolateral side of culture inserts to examine cation selectivity.

Uses

HEPES is a biological buffer. It may be used in isoelectric focusing applications and as a buffer in the quantitative and selective measurement of antigen-antibody reactions. HEPES is reportedly superior to NaHCO3 in controlling pH in tissue and organ culture. HEPES is not recommended for use with the Folin-Ciocalteu protein assay.

Definition

ChEBI: A HEPES that is ethanesulfonate substituted by a 4-(2-hydroxyethyl)piperazin-4-ium-1-yl group at position 2.

General Description

HEPES buffered saline is a tissue culture buffer, which is used to maintain the pH of cell culture. It is water soluble and atmosphere independent. HEPES destroys magnesium in sodium chloride solutions.

Biological Activity

HEPES is a multi purpose HEPES buffer used in cell culture and other biological research. Working pH range in aqueous solution: 6.8 - 8.2. Does not form complexes with metal ions. Used in cell culture media.

Biological Activity

Multi purpose buffer used in biological research.

storage

Room temperature

Purification Methods

Crystallise the acid from hot EtOH and water. It is a useful buffer. [Beilstein 23 V 376.]

HEPES Preparation Products And Raw materials

Raw materials

Preparation Products

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View Lastest Price from HEPES manufacturers

Jinan Finer Chemical Co., Ltd

Product: 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid 7365-45-9
Price: US $0.00/Kg/Drum
Min. Order: 1KG
Purity: 99%
Supply Ability: 200mt
Release date: 2021-11-24

Hebei Zhuanglai Chemical Trading Co.,Ltd

Product: HEPES 7365-45-9
Price: US $25.00/kg
Min. Order: 1kg
Purity: 99%
Supply Ability: 5000kg/week
Release date: 2024-04-26

Chongqing Zhihe Biopharmaceutical Co., Ltd.

Product: HEPES 7365-45-9
Price: US $0.00-0.00/kg
Min. Order: 0.10000000149011612kg
Purity: ≥99%
Supply Ability: 20tons
Release date: 2024-04-18

7365-45-9, HEPESRelated Search:

HEPES 2-[4-(2-HYDROXYETHYL)-1-PIPERAZINYL]ETHANESULFONIC ACID MOLECULAR BIOLOGY GRADE

HEPES 2-[4-(2-HYDROXYETHYL)-1-PIPERAZINYL]ETHANESULFONIC ACID 99+%

N-(2-HYDROXYETHYL)-PIPERAZINE-N'-2-ETHANESULFONIC ACID(4-(2-HYDROXYETHYL)PIPERAZINE-1-ETHANESULFONIC ACID(HEPES-ACID))

Hepes,FreeAcid(N-[2-Hydroxyethyl]Piperaz

1-HeptaneSulfonicAcidSod.SaltGr

N-(2-Hydroxyethyl)Piperazine-N'-(3-EthanesulfonicAcid)

N-(2-Hydroxyethyl)Piperazine-N'-(2-EthanesulfonicAcid)(Hepes)

Hepes,LowSodium,FreeAcid

HEPES free acid, DNase, Rnase and Protease free, for molecular biology, 99+%

Hydroxyethyl)-1-piperazineethyanesulfonic acid, 4-(2-

4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid(HEPES)

2-(4-(2-Hydroxyethyl)piperazin-1-yl)ethanesulfonic acid

4-(2-hydroxyerhyl)piperazine-1-erhaesulfonic acid

HEPES, for biochemistry, 99%

4-(2-Hydroxyethyl)-1-Piperazin

Hydroxyethyl)piperazine-N'-2-ethane sulfonic acid

1-Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)-

hepes solution

HEPES,BIOLOGICALBUFFER

HEPES,BIOLOGICALBUFFER,ULTRAPURE

N-(2-hydroxymethyl)piperazine-N｀-(2-ethanesulfonic acid)(HEPES)

N-2-HYDROXYETHYLPIPERAZINE-N-2-ETHANSULFONICACID(HEPES)

4-(2-Hydroxyethyl)piperazin-1-ylethansulfonsure

2-[4-(2-Hydroxyethyl)-1-piperazine]ethanesulfonic acid HEPES

HEPES / 4-(2-HYDROXYETHYL)-1-PIPERAZINEETHANESULPHONIC ACID

HEPES (4-(2-HYDROXYETHYL)PIPERAZINE-1-ETHANESULFONIC ACID)

HEPES ULTRAPURE

N-2-HYDROXYETHYLPIPERAZINE-N''-2-ETHANE SULPHONIC ACID >99.5%

N-[2-HYDROXYERHYL]PIPERAZINE-N''-ETHANESULFONICACID(HEPES)

HEPES free acid ultrapure

N-2-Hydroxyethylpiperazine-N'-2-ethanesulphonic acid, low sodium >99.5%

2-[4-(2-Hydroxyethyl)-1-piperazinyl]ethanesulfonic Acid [Good's buffer component for biological research]

HEPES(N-[2-Hydroxyethyl]piperazine-N'-[Ethanesulfonic acid]),Free Acid

N'-2-N-2-hydroxyethyl piperazineethanesulfonic acid

HEPES, >=99.5% (titration)

HEPES SynonyMs 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid

HEPES Buffer Solution (1M)

HEPES,4-(2-hydroxyerhyl)piperazine-1-erhanesulfonic acid

HEPES Vetec(TM) reagent grade, 99.5%

4-CHLORO-1-NAPHTHOL ULTRA PURE GRADE

POTASSIUM HYDROXIDE 0,05 MOL DUTY PAID

POTASSIUM HYDROXIDE 0,5MOL/L DUTY PAID

POTASSIUM HYDROXIDE 1 MOL/L DUTY PAID

SELENIUM STAND

MALATE DEHYDROGENASE ULTRA PURE GRADE

PHOSPHOTUNGSTIC ACID HIGH PURITY GRADE

N-[2-HYDROXYETHYL]PIPERAZINE-N'-[2-ETHANESULFONIC ACID];4-(2-HYDROXYETHYL)PIPERAZINE-1-ETHANESULFONIC ACID

4-(2-hydroxyethyl)-1-piperazineethanesulfonicaci

hepesfreeacid

4-(2-HYDROXYETHYL)-1-PIPERAZINEETHANESULFONIC ACID

4-(2-HYDROXYETHYL)PIPERAZINE-1-ETHANESULFONIC ACID

2-[4-(2-HYDROXYETHYL)-1-PIPERAZINE]ETHANESULFONIC ACID

2-[4-(2-HYDROXYETHYL)-1-PIPERAZINYL]ETHANESULFONIC ACID

LABOTEST-BB LT00138109

LABOTEST-BB LT00233156

HEPES

HEPES-ACID

HEPES BUFFER