ChemicalBook > CAS DataBase List > HEPES

HEPES

Product Name
HEPES
CAS No.
7365-45-9
Chemical Name
HEPES
Synonyms
HEPES BUFFER;4-(2-HYDROXYETHYL)-1-PIPERAZINEETHANESULFONIC ACID;hepes solution;HYDROXYETHYLPIPERAZINE ETHANE SULFONIC ACID;Biological Buffer;HEPES buffer Solution;4-(2-HYDROXYETHYL)PIPERAZINE-1-ETHANESULFONIC ACID;4-(2-hydroxyethyl)piperazin-1-ylethanesulphonic acid;2-[4-(2-HYDROXYETHYL)-1-PIPERAZINYL]ETHANESULFONIC ACID;EPES
CBNumber
CB5408557
Molecular Formula
C8H18N2O4S
Formula Weight
238.3
MOL File
7365-45-9.mol
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HEPES Property

Melting point:
234-238 °C
Boiling point:
408℃[at 101 325 Pa]
bulk density
560kg/m3
Density 
1.07 g/mL at 20 °C
vapor pressure 
0Pa at 25℃
refractive index 
n20/D 1.339
storage temp. 
2-8°C
solubility 
H2O: 1 M at 20 °C, clear, colorless
form 
powder
color 
White
PH
5.0-6.5 (1Maqueous solution)
pka
7.5(at 25℃)
PH Range
7.0 - 7.6
biological source
synthetic
Water Solubility 
Soluble
λmax
λ: 260 nm Amax: 0.06
λ: 280 nm Amax: 0.04
Merck 
14,4654
BRN 
883043
Stability:
Stable. Combustible. Incompatible with strong oxidizing agents. Protect from moisture.
InChIKey
JKMHFZQWWAIEOD-UHFFFAOYSA-N
LogP
-3.85 at 20℃
CAS DataBase Reference
7365-45-9(CAS DataBase Reference)
EPA Substance Registry System
1-Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)- (7365-45-9)
Absorption
≤0.05 at 290 in H2O at 33%
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Safety

Hazard Codes 
Xi
Risk Statements 
36/37/38
Safety Statements 
24/25-22-36-26
WGK Germany 
1
RTECS 
TL6809000
TSCA 
Yes
HS Code 
29335995
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Warning
Hazard statements

H302Harmful if swallowed

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P271Use only outdoors or in a well-ventilated area.

P280Wear protective gloves/protective clothing/eye protection/face protection.

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
PHG0001
Product name
HEPES
Packaging
1KIT
Price
$1008
Updated
2025/07/31
Sigma-Aldrich
Product number
RES6003H-B7
Product name
HEPES
Packaging
50kg
Price
$17778
Updated
2025/07/31
Sigma-Aldrich
Product number
H4034
Product name
HEPES
Purity
BioPerformance Certified, ≥99.5% (titration), suitable for cell culture
Packaging
25KG
Price
$6600
Updated
2025/07/31
Sigma-Aldrich
Product number
H3375
Product name
HEPES
Purity
≥99.5% (titration)
Packaging
25KG
Price
$6100
Updated
2025/07/31
Sigma-Aldrich
Product number
H3375
Product name
HEPES
Purity
≥99.5% (titration)
Packaging
50KG
Price
$11500
Updated
2025/07/31
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HEPES Chemical Properties,Usage,Production

Description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). It is typically used in cell culture at concentration between 5mM to 30 mM. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Chemical Properties

White crystalline powder

Uses

Non-toxic to cell. It is used as a hydrogen ion buffer, which can control the constant pH range for long term. The concentration is 10-50mmol/L. Generally, 20mmol/LHEPES in nutrient solution can get buffer ability.

Uses

HEPES is common buffer for biological sciences, particularly used in cell culture to maintain physiological pH. It acts a buffering component, which is used in the preparation of buffers. It is described as one of the best all-purpose buffers available for use in biological research.
HEPES has been used as a component of:
Hank′s balanced salt solution, Dulbecco′s modified eagle′s medium and no-glucose DMEM, which are used for the preparation of tissue slices.
Buffers used in localized conductance measurements on the basolateral side of culture inserts to examine cation selectivity.

Uses

HEPES is a biological buffer. It may be used in isoelectric focusing applications and as a buffer in the quantitative and selective measurement of antigen-antibody reactions. HEPES is reportedly superior to NaHCO3 in controlling pH in tissue and organ culture. HEPES is not recommended for use with the Folin-Ciocalteu protein assay. 

Definition

ChEBI: A HEPES that is ethanesulfonate substituted by a 4-(2-hydroxyethyl)piperazin-4-ium-1-yl group at position 2.

General Description

HEPES buffered saline is a tissue culture buffer, which is used to maintain the pH of cell culture. It is water soluble and atmosphere independent. HEPES destroys magnesium in sodium chloride solutions.

Biological Activity

HEPES is a multi purpose HEPES buffer used in cell culture and other biological research. Working pH range in aqueous solution: 6.8 - 8.2. Does not form complexes with metal ions. Used in cell culture media.

Biological Activity

Multi purpose buffer used in biological research.

Synthesis

103-76-4

1562-00-1

7365-45-9

The general procedure for the synthesis of 4-hydroxyethylpiperazineethanesulfonic acid from N-hydroxyethylpiperazine and sodium 2-hydroxyethylsulfonate is as follows: 0.5 mole of N-hydroxyethylpiperazine, 0.565 mole of sodium 2-hydroxyethylsulfonate, and 500 ml of methanol were added to a 1000 ml four-necked flask fitted with a refluxing condenser tube, and the condensation reaction was carried out under sufficient stirring. The reaction was first carried out at 50 °C for 2.5 minutes, then gradually heated to reflux and continued for 3.0 hours. The reaction was cooled after completion of the reaction to give a reaction mixture containing 4-hydroxyethylpiperazine ethanesulfonate. The yield of 4-hydroxyethylpiperazine ethanesulfonate was 93.6% as analyzed by high performance liquid chromatography. A 20 liter solution of 4-hydroxyethylpiperazine ethanesulfonate at a mass concentration of 15 wt% was diluted to 5 wt% with deionized water under stirring, followed by removal of sodium chloride by dialysis. Then the purified HEPES product was obtained by evaporation concentration, cooling crystallization and filtration. Finally, the product was washed once with anhydrous methanol and dried under vacuum to obtain purified HEPES.A sample of purified HEPES was dissolved and tested for purity, which showed a purity of 99.9%, a yield of 85.6%, a pH of 6.46, and a concentration of chloride ions of 6.8 μg/g.

storage

Room temperature

Purification Methods

Crystallise the acid from hot EtOH and water. It is a useful buffer. [Beilstein 23 V 376.]

References

[1] Patent: CN106905262, 2017, A. Location in patent: Paragraph 0034; 0035; 0047

HEPES Preparation Products And Raw materials

Raw materials

Preparation Products

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HEPES Suppliers

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View Lastest Price from HEPES manufacturers

Hebei Chuanghai Biotechnology Co,.LTD
Product
HEPES 7365-45-9
Price
US $5.00-2.00/KG
Min. Order
1KG
Purity
99%
Supply Ability
10000kg
Release date
2025-06-10
Hebei Chuanghai Biotechnology Co., Ltd
Product
HEPES 7365-45-9
Price
US $10.00/KG
Min. Order
1KG
Purity
99%
Supply Ability
10 mt
Release date
2024-11-13
Jinan Finer Chemical Co., Ltd
Product
4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid 7365-45-9
Price
US $0.00/Kg/Drum
Min. Order
1KG
Purity
99%
Supply Ability
200mt
Release date
2021-11-24

7365-45-9, HEPESRelated Search:

2-Ethylpiperazine 1-Ethylpiperazine 2-Hydroxyethanesulphonic acid 2-(METHYLSULFONYL)ETHANOL Ethyl 2-(Chlorosulfonyl)acetate N-Aminoethylpiperazine 2-Piperidinoethanol Hydroxyethyl starch Piperazine Sodium 2-bromoethanesulphonate Hydroxyethyl Cellulose Calcium dobesilate 1-(2-Hydroxyethyl)piperazine 2-(2-Aminoethylamino)ethanol Sodium isethionate Folic acid Piperazine citrate Ascoric Acid Hepes Buffer Hepes Buffered Saline Hepes Solution

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