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Tricine

Descriptioin Reference

Product Name: Tricine
CAS No.: 5704-04-1
Chemical Name: Tricine
Synonyms: TRIS-HCL BUFFER;1389475;TROMETHAMINE HYDROCHLORIDE;Ticine;Tricine buffer, free acid;TRI(HYDROXYMETHYL) AMINO METHANE HYDROCHLORIDE;TRICINE;TRIClNE;n-Tricine-D8;Tricine >99%
CBNumber: CB5853856
Molecular Formula: C6H13NO5
Formula Weight: 179.17
MOL File: 5704-04-1.mol

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Tricine Property

Melting point:: 186-188 °C (dec.)
Boiling point:: 311.69°C (rough estimate)
Density: 1.05 g/mL at 20 °C
bulk density: 500kg/m3
vapor pressure: 0.002-0.004Pa at 20-25℃
refractive index: 1.4240 (estimate)
storage temp.: Keep in dark place,Inert atmosphere,Room temperature
solubility: H₂O: 4 M at 20 °C, clear, colorless
form: crystalline
color: clear colorless (40 % (w/w) in H₂O) solution
PH Range: 7.4 - 8.8
Odor: Odorless
PH: 4.0-6.0 (1M in H2O)
pka: 8.1(at 25℃)
biological source: synthetic
Water Solubility: soluble
Merck: 14,9651
BRN: 1937804
Stability:: Stable.
LogP: -2.87 at 25℃
CAS DataBase Reference: 5704-04-1(CAS DataBase Reference)
EPA Substance Registry System: Glycine, N-[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]- (5704-04-1)
Absorption: ≤0.05 at 280 in H2O at 1M
≤0.1 at 260 in H2O at 1M

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Safety

Hazard Codes: Xi
Risk Statements: 36/37/38
Safety Statements: 26-36-36/37/39
WGK Germany: 3
F: 3
TSCA: Yes
HS Code: 29052900

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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word: Warning
Hazard statements: H320Causes eye irritation
Precautionary statements: P403Store in a well-ventilated place.

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N-Bromosuccinimide Price

Sigma-Aldrich

Product number: T17917
Product name: Tricine
Purity: ≥99% (titration), reagent grade, crystalline
Packaging: 100G
Price: $81.4
Updated: 2025/07/31

Sigma-Aldrich

Product number: T17917
Product name: Tricine
Purity: ≥99% (titration), reagent grade, crystalline
Packaging: 500G
Price: $375
Updated: 2025/07/31

Sigma-Aldrich

Product number: T17917
Product name: Tricine
Purity: ≥99% (titration), reagent grade, crystalline
Packaging: 1KG
Price: $498
Updated: 2025/07/31

Sigma-Aldrich

Product number: T17917
Product name: Tricine
Purity: ≥99% (titration), reagent grade, crystalline
Packaging: 5KG
Price: $1681.16
Updated: 2025/07/31

Sigma-Aldrich

Product number: T17917
Product name: Tricine
Purity: ≥99% (titration), reagent grade, crystalline
Packaging: 10KG
Price: $3232.59
Updated: 2025/07/31

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Tricine Chemical Properties,Usage,Production

Descriptioin

Tricine is a zwitterionic amino acid. It is a white crystalline powder. It was first prepared by Good to buffer chloroplast reaction.1 Tricine is a commonly used electrophoresis buffer and is also used in resuspension of cell pellets^.1,2 Attributing to its higher negative charge than glycine, it can migrate faster. At the same time, its high ionic strength results in more ion movement and less protein movement. Therefore, it works as a buffer component for separation of low molecular weight peptides.³

Reference

S. R. Haider, B. L. Sharp, H. J. Reid, A comparison of Tris‐glycine and Tris‐tricine buffers for the electrophoretic separation of major serum proteins, Journal of Separation Science, 2011, vol. 34, pp. 2463-2467
H. Schägger, G. von Jagow, Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa, Analytical Biochemistry, 1987, vol. 166, pp. 368-379

Chemical Properties

White/clear crystalline powder

Uses

Good's buffers Tricine is used as an electrophoresis buffer and involved in the separation of low molecular weight proteins and peptides. It operates in the pH range between 7.4 and 8.8. It acts as an effective scavenger of hydroxyl radicals during the study of radiation-induced membrane damage. It is also used in resuspension of cell pellets. It is a zwitterionic amino acid commonly useful in the pH range of 7.4-8.8 and the pKa is 8.05 at 25°C. This buffer is also used as an eluent in asymmetrical flow field-flow fractionation (AFFFF).

Definition

ChEBI: N-tris(hydroxymethyl)methylglycine is a Good's buffer substance, pKa = 8.15 at 20 ℃. It is functionally related to a member of tris and a glycine. It is a tautomer of a N-tris(hydroxymethyl)methylammonioacetate.

General Description

Tricine and its salts are used as biological buffers to control the pH of body fluids in vitro and in vivo. It acts as a precursor for polymers, oxazolones (with carboxylic acids), and oxazolidines (with aldehydes). Trizma can serve as an alkalizing agent in treating acidosis of the blood.

Biological Activity

Electrophoresis buffer. Working pH range: 7.4 - 8.8. Commonly used in SDS-PAGE to seperate peptides. Also used in other electrophoresis methods, high performance liquid chromatography and ion exchange chromatopgraphy.

Purification Methods

Crystallise Tricine from EtOH and water. [Good et al. Methods Enzymol 24B 53 1968, McGothlin & Jordan Analyt Lett 9 245 1976, Beilstein 18 III/IV 3454.]

Tricine Preparation Products And Raw materials

Raw materials

Preparation Products

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View Lastest Price from Tricine manufacturers

Shaanxi Dideu Medichem Co. Ltd

Product: 10x Tris-Tricine-SDS Premixed Powder 5704-04-1
Price: US $0.00-0.00/G
Min. Order: 100G
Purity: 10x Tris-Tricine-SDS Premixed Powder
Supply Ability: KG
Release date: 2025-09-10

Shaanxi Dideu New Materials Co. Ltd

Product: Tricine 5704-04-1
Price: US $0.00/kg
Min. Order: 1kg
Purity: 99%
Supply Ability: 10000KGS
Release date: 2025-04-29

WUHAN FORTUNA CHEMICAL CO., LTD

Product: Tricine 5704-04-1
Price: US $0.00-0.00/Kg/Drum
Min. Order: 1KG
Purity: 99%-101%
Supply Ability: 5 tons
Release date: 2021-05-27

5704-04-1, TricineRelated Search:

CHLOROPHOSPHONAZO III Bensulfuron methyl Methylparaben Ethylparaben Ethanol Sarcosine Di(trimethylol propane) Trimethylolpropane Kresoxim-methyl ISOXADIFEN-ETHYL Ethyl acetate Ethyl acrylate 1,1,1-Tris(hydroxymethyl)ethane Methyl acrylate Sodium hydroxymethylglycinate Tris(hydroxymethyl)aminomethane Methyl TRIS-TRICINE BUFFER

LABOTEST-BB LT00454966

n-[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]-glycin

TRICINE

TRI(HYDROXYMETHYL) AMINO METHANE HYDROCHLORIDE

TRIMETHYLOLAMINOMETHANE HYDROCHLORIDE

TRIClNE

E-Z 96 SIZE SELECT-IT (1 X 96)

TRIZMA(R) HYDROCHLORIDE

TROMETHANE HCL

TROMETHAMINE HYDROCHLORIDE

N-[2-HYDROXY-1,1-BIS(HYDROXYMETHYL)ETHYL]GLYCINE

MOLE-READY-TRIS

N-[TRIS(HYDROXYMETHYL)METHYL]GLYCINE

SPECTRIS(R) HYDROCHLORIDE

SPECTRIS(TM) HYDROCHLORIDE

N-(tri(hydroxymethyl)methyl)glycine

Tricine, Molecular Biology Grade N-Tris(hydroxymethyl)methylglycine, Molecular Biology Grade

Tricine, ULTROL Grade

TRICINE, BIOTECHNOLOGY PERFORMANCE CERTI FIED

TRICINE SIGMAULTRA

TRICINE 1M SOLUTION, BIOTECHNOLOGY PERFO

Tricine(N-Tris-(Hydroxymethyl)

Tricine, 98+%

Tricine, for biochemistry, 98%

TRICINE, BIOPERFORMANCE CERTIFIED, C

Tricine, for biochemistry, 99+%

n-Tricine-D8

N-[2-hydroxy-1,1-bis(hydroxyme

Glycine, N-2-hydroxy-1,1-bis(hydroxymethyl)ethyl-

N-tris(Hydroxymethyl)methylglycine (TRICINE)

TRICINE ULTRAPURE

Tricine >99%

N-TRIS(HYDROXYMETHYL) METHLGLYCINE extrapure

tris hydrochloride solution

Trizma(R) hydrochloride solution

1,3-Dihydroxy-2-(hydroxymethyl)propan-2-ylaminoacetic acid

Carboxymethylaminomethylidynetrimethanol

1389475

OmniPur TRICINE - CAS 1389475 - Calbiochem

Tricine, ULTROL Grade - CAS 1389475 - Calbiochem

2-{[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino}acetic acid

2-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]ammonio]acetate

TRIS(HYDROXYMETHYL)AMINOMETHANE, HCL

TRIS(HYDROXYMETHYL)METHYLAMINE HYDROCHLORIDE

TRIS(HYDROXYMETHYL)METHYLAMMONIUM CHLORIDE

TRIS(HYDROXYMETHYL) METHYLGLYCINE

TRISMAT(R) HYDROCHLORIDE

TRISMAT(TM) HYDROCHLORIDE

TROMETAMOL HYDROCHLORIDE

N-[1,1-Bis(hydroxymethyl)-2-hydroxyethyl]glycine

N-[1,3-Dihydroxy-2-(hydroxymethyl)propan-2-yl]glycine

Tricine Vetec(TM) reagent grade, >=99%

Tricine,99+%,for biochemistry

Tricine,98%,for biochemistry

Tricine&nbsp

Ticine

Tricine, for biocheMistry, 98% 100GR

Tricine, for biocheMistry, 98% 250GR