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β-Galactosidase

Product Name
β-Galactosidase
CAS No.
9031-11-2
Chemical Name
β-Galactosidase
Synonyms
s2107;sl396;LACTASE;oryzatym;maxilact;lactozym;sumiklat;hydrolact;tilactase;LACTASE F
CBNumber
CB6123518
Molecular Formula
NULL
Formula Weight
0
MOL File
Mol file
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β-Galactosidase Property

Melting point:
100 °C
Boiling point:
100 °C(Press: 800 Torr)
Density 
1.14 g/cm3(Temp: 4 °C)
storage temp. 
-20°C
solubility 
1% acetic acid: 1 mg/mL
form 
powder
color 
slightly yellow
Water Solubility 
Soluble in water
EPA Substance Registry System
Galactosidase, .beta.- (9031-11-2)
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Safety

Hazard Codes 
Xn
Risk Statements 
22
Safety Statements 
24/25
WGK Germany 
3
RTECS 
LW5840000
3-10
HS Code 
35079090
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Danger
Hazard statements

H334May cause allergy or asthma symptoms or breathing difficulties if inhaled

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P285In case of inadequate ventilation wear respiratory protection.

P304+P341IF INHALED: If breathing is difficult, remove victim to fresh air and keep at rest in a position comfortable for breathing.

P342+P311IF experiencing respiratory symptoms: call a POISON CENTER or doctor/physician.

P501Dispose of contents/container to..…

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
G6008
Product name
β-Galactosidase from Escherichia coli
Purity
Grade VI, lyophilized powder, ≥250?units/mg protein
Packaging
1000units
Price
$99.7
Updated
2024/03/01
Sigma-Aldrich
Product number
G6008
Product name
β-Galactosidase from Escherichia coli
Purity
Grade VI, lyophilized powder, ≥250?units/mg protein
Packaging
5000units
Price
$294
Updated
2024/03/01
Sigma-Aldrich
Product number
G5635
Product name
β-Galactosidase from Escherichia coli
Purity
Grade VIII, lyophilized powder, ≥500?units/mg protein
Packaging
1000units
Price
$114
Updated
2024/03/01
Sigma-Aldrich
Product number
G6008
Product name
β-Galactosidase from Escherichia coli
Purity
Grade VI, lyophilized powder, ≥250?units/mg protein
Packaging
10000units
Price
$504
Updated
2024/03/01
Sigma-Aldrich
Product number
G5635
Product name
β-Galactosidase from Escherichia coli
Purity
Grade VIII, lyophilized powder, ≥500?units/mg protein
Packaging
3000units
Price
$258
Updated
2024/03/01
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β-Galactosidase Chemical Properties,Usage,Production

Chemical Properties

White to tan powder

Uses

β-Galactosidase is conjugated to an antibody which specifically recognizes a target molecule (enzyme immunoassay or EIA). 1 β-Galactosidase is also used as a reporter enzyme to monitor the level of gene expression of a promoter. 2 It may be used as a positive control protein with anti-β-galactosidase antibodies.

Uses

β-Galactosidase is used in the enzymatic assays in the synthesis of imidazolo-pyrrolidinoses.

Uses

β-Galactosidase may be used for derivatization, such as an enzyme label for IgG, without prior dialysis or gel filtration.

Uses

β-Galactosidase from bovine testes was used for coupling to Sepharose.

Uses

β-Galactosidase was used for reversed-phase (RP) adsorption. 1 It was also used in the hydrolysis of whey lactose.

Uses

β-galactosidase was used in the production of a stabilized, single reagent for alcohol analysis.

Definition

ChEBI: Beta-D-galactose is a D-galactopyranose having beta-configuration at the anomeric centre. It has a role as an epitope and a mouse metabolite. It is an enantiomer of a beta-L-galactose.

brand name

Replagal (Transkaryotic Therapies).

Biotechnological Production

b-D-Galactosidase (b-D-galactoside galactohydrolase, EC 3.2.1.23) hydrolyses the glycosidic bond of the disaccharide lactose into its monomers glucose and galactose. Therefore, b-D-galactosidase is also known as lactase. b-D-Galactosidases can be found throughout nature. Commercial enzymes are mainly of fungal origin of the genera Kluyveromyces (yeast) and Aspergillus (filamentous ascomycete). Although several b-D-galactosidases are available on the market, new efforts are still conducted to improve the enzymatic properties by site-directed mutagenesis or to find new b-D-galactosidase genes by screening metagenome databases.
From a technological point of view, the milk sugar lactose is hydrolyzed because of the resulting increase of sweetness and the reduced susceptibility to crystallization during spray drying of milk and whey. Nevertheless, the most apparent application is the production of ‘‘lactose-free’’ milk products, which enables the consumption of dairy products by lactose-intolerant people.
For industrial processes, b-D-galactosidases can be applied by immobilization of the enzyme on carriers, such as cellulose, alginate, or other polymers, for hydrolysis of milk or whey products. Conversion rates of lactose in batch and continuous operation mode by immobilized b-D-galactosidase might reach 95 %. To reach even lower lactose concentrations of less than 0.01 %, a combination of b-D-galactosidase activity and ultrafiltration as well as nanofiltration methods can be used. Besides the degradation of substances that lead to food intolerances, the elimination of food allergens by means of a specific degradation of allergenic epitopes is another promising application of enzymes.

General Description

The GLB1 (β-Galactosidase) gene is mapped to human chromosome 3p22.3. The encoded protein belongs to glycosyl hydrolase 35 family and is localized to lysosomes.

Biochem/physiol Actions

Releases β(1→3)- and β(1→6)-linked galactose from the non-reducing end of complex oligosaccharides.

Purification Methods

It is purified 600-fold by (NH4)2SO4 precipitation, acetone fractionation and affinity chromatography on agarose substituted with terminal thio-.-galactopyranosyl residues. [Distlern & Jourdian J Biol Chem 248 6772 1973.]

β-Galactosidase Preparation Products And Raw materials

Raw materials

Preparation Products

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β-Galactosidase Suppliers

Xi'an Kono chem co., Ltd.
Tel
29-84385017-8016 15891718909
Fax
15891718909
Email
sales@konochemical.com
Country
China
ProdList
141
Advantage
58
Shanghai Baoman Biotechnology Co., Ltd.
Tel
021-62130998
Fax
021-54438215
Email
baomanbio@163.com
Country
China
ProdList
108
Advantage
55
Amano Enzyme Manufacturing(China),Ltd. Shanghai Branch
Tel
021-62490810 13917934292
Email
shanghai@amano-enzyme.com.cn
Country
China
ProdList
44
Advantage
58
J & K SCIENTIFIC LTD.
Tel
010-82848833 400-666-7788
Fax
86-10-82849933
Email
jkinfo@jkchemical.com
Country
China
ProdList
94657
Advantage
76
Beijing HwrkChemical Technology Co., Ltd
Tel
010-89508211 18501085097
Fax
010-89508210
Email
sales.bj@hwrkchemical.com
Country
China
ProdList
8418
Advantage
55
Adamas Reagent, Ltd.
Tel
400-6009262 16621234537
Fax
021-64823266
Email
chenyj@titansci.com
Country
China
ProdList
14103
Advantage
59
Shanghai Hanhong Scientific Co.,Ltd.
Tel
021-54306202 13764082696
Email
info@hanhongsci.com
Country
China
ProdList
42958
Advantage
64
Sichuan Kulinan Technology Co., Ltd
Tel
400-1166-196 18981987031
Fax
028-84555506 800101999
Email
cdhxsj@163.com
Country
China
ProdList
11707
Advantage
57
Tianjin heowns Biochemical Technology Co., Ltd.
Tel
400 638 7771
Email
sales@heowns.com
Country
China
ProdList
14435
Advantage
57
Sinopharm Chemical Reagent Co,Ltd.
Tel
86-21-63210123
Fax
86-21-63290778 86-21-63218885
Email
sj_scrc@sinopharm.com
Country
China
ProdList
9815
Advantage
79
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View Lastest Price from β-Galactosidase manufacturers

WUHAN FORTUNA CHEMICAL CO., LTD
Product
β-Galactosidase 9031-11-2
Price
US $0.00/Kg/Drum
Min. Order
1KG
Purity
≥5000ALU/g food grade
Supply Ability
1000kgs
Release date
2021-08-18
BINBO BIOLOGICAL CO.,LTD
Product
Fungal beta-Galactosidase 9031-11-2
Price
US $0.00/kg
Min. Order
1kg
Purity
99.8%
Supply Ability
1000 kg
Release date
2024-03-26
Hebei Weibang Biotechnology Co., Ltd
Product
beta-Galactosidase 9031-11-2
Price
US $0.00-0.00/KG
Min. Order
1KG
Purity
99%
Supply Ability
500000kg
Release date
2024-10-29

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