2-(2-[4-(1,1,3,3-Tetramethylbutyl)phenoxy]ethoxy)ethanol Property

Melting point:

0.05°C

Boiling point:

586.77°C (rough estimate)

Density 

1.06 g/mL at 20 °C

vapor pressure 

<0.01 hPa (20 °C)

refractive index 

n20/D 1.492

Flash point:

>230 °F

storage temp. 

Store at +5°C to +30°C.

solubility 

Chloroform (Slightly), DMSO (Slightly), Methanol (Slightly), Water (Slightly)

form 

Liquid

color 

Yellow

PH

5.0-8.0 (10g/l, H2O, 20℃)

Water Solubility 

Miscible with water.

Merck 

13,6793

BRN 

2315025

Dielectric constant

9.8（29.0℃）

CAS DataBase Reference

9036-19-5(CAS DataBase Reference)

EPA Substance Registry System

Polyethylene glycol mono(1,1,3,3-tetramethylbutyl)phenyl ether (9036-19-5)

Safety

Hazard Codes 

Xn,Xi,N

Risk Statements 

41-38-22-51/53-52/53

Safety Statements 

26-39-61

RIDADR 

UN 3082 9 / PGIII

WGK Germany 

2

RTECS 

MD0907600

F 

3-8-10-23

TSCA 

Yes

HS Code 

34021300

Hazardous Substances Data

9036-19-5(Hazardous Substances Data)

Toxicity

LD50 orally in Rabbit: 1900 - 5000 mg/kg LD50 dermal Rabbit > 3000 mg/kg

Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Danger

Hazard statements

H315Causes skin irritation

H318Causes serious eye damage

H410Very toxic to aquatic life with long lasting effects

Precautionary statements

P273Avoid release to the environment.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P302+P352IF ON SKIN: wash with plenty of soap and water.

2-(2-[4-(1,1,3,3-Tetramethylbutyl)phenoxy]ethoxy)ethanol Chemical Properties,Usage,Production

Uses

Triton^? X-100 has been used:

• In immunohistochemistry for staining the Flat-mount retinas
• Along with ice-cold PBS (phosphate buffered saline) in suspension of cells for cell DNA analysis and Annexin V assay
• To permeabilise cells during Immunofluorescent microscopic studies
• As a positive control in LDH assay to determine the cell membrane integrity
• For estimating the lipase activity in postheparin plasma by using modified Belfrage and Vaughan radioenzymatic procedure
• For the preparation of outer membrane protein exctract
• As a component of extraction buffer along with tris-HCl, NaCl, CaCl₂, ZnCl₂, Brij 35 for homogenization of mice lung cells
• In the treatment of tissue sections for Immunofluorescence labeling

Uses

0·3% Triton^? X-100 in TBS has been used for permeabilization of retinas before immunostaining. 0.5% Triton X-100 has been used for astrocyte cell lysis.

Uses

Triton^?-X has been used:

• In the permeabilization of cells for immunofluorescence staining
• As a component of lysis buffer in western blot analysis
• As a component of Tris-buffered saline for the preparation of cell sections in Immunogold labelling for electron microscope

General Description

Octophenolpoly(ethyleneglycolether)_n
Especially used for purification in membrane research
Triton X-100 is a water soluble, non-ionic surfactant. It exhibits a strong hydrogen bonding with water molecule in temperature dependent manner. It has a wide application area such as in isolation and solubilization of protein, DNA extraction and emulsification.

Biochem/physiol Actions

Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.

Safety Profile

Moderately toxic by ingestion.When heated to decomposition it emits acrid smoke andirritating fumes.

Safety Profile

Moderately toxic by ingestion. Aneye irritant. When heated to decomposition it emits acridsmoke and irritating fumes.