Z-LEU-LEU-GLU-AMC
- Product Name
- Z-LEU-LEU-GLU-AMC
- CAS No.
- 348086-66-8
- Chemical Name
- Z-LEU-LEU-GLU-AMC
- Synonyms
- Z-ILE-AMC;Z-LLE-AMC;Z-LLG-AMC;Z-LEU-LEU-GLU-MCA;Z-LEU-LEU-GLU-AMC;Z-LEU-LEU-GLY-AMC;CBZ-LEU-LEU-GLU-AMC;Proteasome Substrate II;Cbz-Leu-Leu-Glu-AMC, Z-LLE-AMC;PROTEASOME SUBSTRATE II (FLUOROGENIC)
- CBNumber
- CB6298942
- Molecular Formula
- C35H44N4O9
- Formula Weight
- 664.75
- MOL File
- 348086-66-8.mol
Z-LEU-LEU-GLU-AMC Property
- Boiling point:
- 967.8±65.0 °C(Predicted)
- Density
- 1.253±0.06 g/cm3(Predicted)
- storage temp.
- -20°C
- solubility
- DMSO: soluble
- form
- solid
- pka
- 4.43±0.10(Predicted)
- color
- Solid lyophilized powder
- Sequence
- {Z}-Leu-Leu-Glu-{AMC}
Safety
- Safety Statements
- 22-24/25
- WGK Germany
- 3
N-Bromosuccinimide Price
- Product number
- C0483
- Product name
- Z-Leu-Leu-Glu-7-amido-4-methylcoumarin
- Purity
- ≥95%, solid
- Packaging
- 5MG
- Price
- $426
- Updated
- 2025/07/31
- Product number
- 539141
- Product name
- Proteasome Substrate II, Fluorogenic
- Purity
- Fluorogenic proteasome substrate.
- Packaging
- 5 mg
- Price
- $288
- Updated
- 2025/07/31
- Product number
- 10008117
- Product name
- Z-LLE-AMC
- Purity
- ≥95%
- Packaging
- 1mg
- Price
- $44
- Updated
- 2024/03/01
- Product number
- 10008117
- Product name
- Z-LLE-AMC
- Purity
- ≥95%
- Packaging
- 5mg
- Price
- $130
- Updated
- 2024/03/01
- Product number
- 10008117
- Product name
- Z-LLE-AMC
- Purity
- ≥95%
- Packaging
- 10mg
- Price
- $235
- Updated
- 2024/03/01
Z-LEU-LEU-GLU-AMC Chemical Properties,Usage,Production
Description
Z-LLE-AMC is a fluorogenic substrate for the caspase-like post-glutamate peptide hydrolase of the 26S proteasome or 20S proteolytic core. Caspase-like activity can be quantified by fluorescent detection of free AMC (also known as 7-amino-4-methylcoumarin), which is excited at 340-360 nm and emits at 440-460 nm. Z-LLE-AMC is typically used in cell lysates after experimental treatment.
Uses
Z-Leu-Leu-Glu-7-amido-4-methylcoumarin has been used as a fluorogenic substrate for peptidyl glutamyl peptide hydrolase (PGPH) in rat soleus muscle, neonatal cardiomyocytes and rat brain samples.
Uses
A fluorogenic substrate for the peptidylglutamyl-peptide hydrolysing (caspase-like) activity of the proteasome which may be stimulated in the presence of Mg2+ ions and rapidly inactivated by N-acetylimidazole
General Description
Z-Leu-Leu-Glu-7-amido-4-methylcoumarin is a fluoropeptide. It acts as a substrate for caspase-like activity.
Biochem/physiol Actions
Fluorogenic substrate for the measurement of the peptidylglutamyl-peptide hydrolyzing activity of the 20S proteasome.
storage
+4°C
References
[1] MARIAN ORLOWSKI. Regulation of the peptidylglutamyl-peptide hydrolyzing activity of the pituitary multicatalytic proteinase complex[J]. Biochemistry Biochemistry, 1991, 30 24: 5999-6005. DOI: 10.1021/bi00238a025
[2] ELKE GEIER. A Giant Protease with Potential to Substitute for Some Functions of the Proteasome[J]. Science, 1999, 283 5404. DOI: 10.1126/science.283.5404.978
[3] MOHAMED-AMINE HAMOUDA. The small heat shock protein B8 (HSPB8) confers resistance to bortezomib by promoting autophagic removal of misfolded proteins in multiple myeloma cells.[J]. Oncotarget, 2014, 5 15: 6252-6266. DOI: 10.18632/oncotarget.2193
[4] LAURA TADLOCK. Transforming growth factor-beta inhibition of proteasomal activity: a potential mechanism of growth arrest.[J]. American journal of physiology. Cell physiology, 2003, 285 2: C277-85. DOI: 10.1152/ajpcell.00550.2002
Z-LEU-LEU-GLU-AMC Preparation Products And Raw materials
Raw materials
Preparation Products
Z-LEU-LEU-GLU-AMC Suppliers
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- United Kingdom
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