DL-Dithiothreitol
- Product Name
- DL-Dithiothreitol
- CAS No.
- 3483-12-3
- Chemical Name
- DL-Dithiothreitol
- Synonyms
- DTT;DITHIOTHREITOL;DL-1,4-DITHIOTHREITOL;1,4-DITHIOTHREITOL;1,4-DITHIO-DL-THREITOL;Sputolysin;Cleland's reagent;DTT-MBG;DITHIOTHRIETOL;cis-2,3-Dihydroxy-1,4-butanedithiol
- CBNumber
- CB6342919
- Molecular Formula
- C4H10O2S2
- Formula Weight
- 154.25
- MOL File
- 3483-12-3.mol
DL-Dithiothreitol Property
- Melting point:
- 41-44 °C(lit.)
- Boiling point:
- 125 °C
- alpha
- -0.2～+0.2°(20℃/D)(c=5,H2O)
- Density
- 1.04 g/mL at 20 °C
- vapor density
- 5.3 (vs air)
- vapor pressure
- 0.019-0.29Pa at 20-50℃
- refractive index
- 1.5200 (estimate)
- Flash point:
- >230 °F
- storage temp.
- -20°C (or 4°C short term).
- solubility
- H2O: 50 mg/mL, clear, colorless
- form
- Powder
- pka
- pK1:8.9 (25°C)
- color
- White
- PH
- 4.0-6.0 (20-25℃, 0.1m in H2O)
- Odor
- Unpleasant Odor
- PH Range
- 4 - 6 at 15,4 g/l at 25 °C
- Water Solubility
- freely soluble
- Sensitive
- Air Sensitive
- λmax
- λ: 260 nm Amax: 0.400
λ: 280 nm Amax: 0.100 - Merck
- 14,3376
- BRN
- 1719757
- Stability:
- Stability Stable, but heat sensitive. Incompatible with strong oxidizing agents. Keep frozen at -20 to -10 C.
- InChIKey
- VHJLVAABSRFDPM-UHFFFAOYSA-N
- LogP
- 0.07 at 25℃ and pH5
- CAS DataBase Reference
- 3483-12-3(CAS DataBase Reference)
- NIST Chemistry Reference
- 2,3-Butanediol, 1,4-dimercapto-, (r*,r*)-(3483-12-3)
- EPA Substance Registry System
- 1,4-Dithiothreitol (3483-12-3)
Safety
- Hazard Codes
- Xn
- Risk Statements
- 25-36/37/38-22-20/21/22
- Safety Statements
- 26-45-37/39-36
- RIDADR
- UN 3335
- WGK Germany
- 3
- RTECS
- EK1610000
- F
- 3-10-16-23
- TSCA
- Yes
- HS Code
- 29309099
- Hazardous Substances Data
- 3483-12-3(Hazardous Substances Data)
Hazard and Precautionary Statements (GHS)
- Symbol(GHS)
-
- Signal word
- Danger
- Hazard statements
-
H302Harmful if swallowed
H315Causes skin irritation
H318Causes serious eye damage
- Precautionary statements
-
P264Wash hands thoroughly after handling.
P264Wash skin thouroughly after handling.
P270Do not eat, drink or smoke when using this product.
P280Wear protective gloves/protective clothing/eye protection/face protection.
P301+P312IF SWALLOWED: call a POISON CENTER or doctor/physician IF you feel unwell.
P302+P352IF ON SKIN: wash with plenty of soap and water.
P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.
N-Bromosuccinimide Price
- Product number
- DTT-RO
- Product name
- DTT
- Purity
- 1,4-Dithiothreitol
- Packaging
- 2g
- Price
- $89.3
- Updated
- 2024/03/01
- Product number
- DTT-RO
- Product name
- DTT
- Purity
- crystalline powder, =97% (Ellman′s reagent), Mr 154.3
- Packaging
- 10G
- Price
- $251
- Updated
- 2024/03/01
- Product number
- DTT-RO
- Product name
- DTT
- Purity
- 1,4-Dithiothreitol
- Packaging
- 25g
- Price
- $547
- Updated
- 2024/03/01
- Product number
- 646563
- Product name
- DL-Dithiothreitol solution
- Purity
- 1?M in H2O
- Packaging
- 10x0.5mL
- Price
- $107
- Updated
- 2024/03/01
- Product number
- 233155
- Product name
- Cleland’s Reagent - CAS 3483-12-3 - Calbiochem
- Packaging
- 5G
- Price
- $149
- Updated
- 2024/03/01
DL-Dithiothreitol Chemical Properties,Usage,Production
Chemical Properties
Dithiothreitol (DTT), also known as Cleland's reagent, is a small-molecule redox reagent . Its oxidized form is a disulfide-bonded 6-membered ring. DTT has an epimeric ('sister') compound, dithioerythritol (DTE).
DTT is a white crystalline powder. It is highly soluble in water (clear solution, OD<0.05 at 0.02M), but also in ethanol, chloroform, ether and ethyl acetate.
DTT is an unusually strong reducing agent, with a redox potential of -0.33 V at pH 7. The pKa of thiol groups is typically ~8.3.
The reduction of a typical disulfide bond proceeds by two sequential thiol-disulfide exchange reactions.
Uses
Dithiothreitol is a redox reagent commonly used as a reducing agent for thiolated DNA. Dithiothreitol is also used to reduce the disulfide bonds of proteins.
Uses
DL-dithiothreitol (DTT) is a sulfhydryl compound that acts both as a reagent reducing disulfide bonds and as a protein denaturant on staphylococcal biofilm (Wu et al. 2011). Thanks to these properties, DTT is routinely used in clinical microbiology to liquefy respiratory specimens, but it has also been proven effective to detach biofilm from orthopaedic prosthesis (Drago et al. 2012, 2013). Since it is crucial to discriminate implant-related infections from aseptic loosening in orthopaedics, and because of the difficulty to diagnose subclinical infections, a prompt diagnosis of indwelling device-related infection is important for successful treatments (Borens et al. 2013). A fast microbiological diagnosis and bacterial identification should be recommended in order to set up a specific antimicrobial therapy. According to this strategy, the bacterial detachment from biofilm on explants should be accelerated by supporting the pathogen viability.
Definition
ChEBI: 1,4-dithiothreitol is the threo-diastereomer of 1,4-dimercaptobutane-2,3-diol. It has a role as a reducing agent, a chelator and a human metabolite. It is a dithiol and a 1,4-dimercaptobutane-2,3-diol.
Application
Dithiothreitol (DTT) is a water-soluble reducing reagent used for various applications in biotechnology, biology and biochemistry :
reduces quantitatively disulfides, generating sulfhydryls (used typically at 1-10mM for protein SS reduction)
reduction of proteins before SDS-PAGE analysis, studies of protein structure and function (Kaji 1993)
keep sulfhydryl groups of biomolecules in the reduced state - protects biomolecules in various applications (enzymes or receptors, living cells under ionizing radiations)
prevents the fading of fluorescence such as FITC labeled conjugates (Picciolo 1984)
General Description
1,4-Dithiothreitol (DTT) is the threo isomer of 2,3-dihydroxy-1,4-dithiolbutane, and an isomer of 1,4-dithioerythritol. DTT is used in molecular biology to maintain sulfhydryl (-SH) groups in the reduced state and for quantitative reduction of disulfide (-S-S-) groups, as described by Cleland in his pioneering publication from the 1960's. Its usefulness as an reducing agent stems from its water solubility and reduced odor compared to previous thiol compounds.
DTT is oxidized to the cyclic disulfide, and thereby ensures the reduction of other disulfides in solution. The disulfide reduction is complete in minutes at pH 8. DTT is less pungent and less toxic than 2-mercaptoethanol. Typically, a 7-fold lower concentration of DTT (100 mM) is required compared to 2-mercaptoethanol (5% v/v, 700 mM).
Biochem/physiol Actions
DL-Dithiothreitol denatures proteins by reducing the disulphide bonds and reducing them to SH groups. It also prevents intramolecular and intermolecular disulfide bond formation between cysteine residues of proteins.
Toxicology
The thiol-containing compound Dithiothreitol (DTT) is toxic to cultured cells by inducing the generation of reactive oxygen species that ultimately cause cell death. DTT treatment triggers the transcription of the canonical apoptosis regulators grim, hid, and rpr at low amounts. The amplitude of this induction declines with elevating DTT amounts. Through Live microscopy, Wang et al. have observed apoptotic cells, especially in the gut of DTT-treated flies. In parallel, low DTT amounts also activate the expression of the cuticle barrier component gene snsl. This indicates that a physical defense response is launched upon DTT contact. This combined measure is seemingly successful in preventing fly death. The expression of several known detoxification genes, including cyp6a2, cyp6a8, cyp12d1, and GstD2, is also enhanced through DTT contact[1].
References
[1] Yiwen Wang . “Toxicity of Dithiothreitol (DTT) to Drosophila melanogaster.” Toxicology Reports 8 (2021): Pages 124-130.
Synthesis
The most widely used DL-Dithiothreitol (DTT) synthetic method is to be raw material with Soviet Union's moss sugar alcohol, use potassium permanganate oxidation earlier, obtains the sulfo-intermediate again under the thioacetic acid effect, and this sulfo-intermediate of hydrolysis obtains DTT at last.
Yu discloses a synthesizing method of DL-Dithiothreitol.1,4-butylene glycol is used as an initiating raw material and undergoes an addition reaction with bromine at first to obtain 2,3-dibromo-1,4-butylene glycol; hydrolyzation under the catalyzation of alkali is carried out to obtain dioxirane; addition reaction with thioacetic acid is carried out to obtain dithiothreitol diacetate; and finally hydrolyzation under the catalyzation of alkali is carried out to obtain the DL-Dithiothreitol.
storage
Store lyophilized at 4°C, desiccated. In lyophilized form, the chemical is stable for 12 months. Once in solution, store at -20°C and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.
DTT solutions should be prepared fresh daily. If improperly stored (including room temperature and solution forms) its reducing ability may be reduced. Exposure to air should be minimized, even though DTT has a lower tendency to be oxidized directly by air than other reducing agents.
Recorded half-life of DTT solutions at various pH and temperatures (all are in M potassium phosphate buffer):
pH 6.5 @ 20°C= 40 hours
pH 7.5 @ 20°C = 10 hours
pH 8.5 @ 20°C = 1.4 hours
pH 8.5 @ 0°C = 11 hours
pH 8.5 @ 40°C = 0.2 hours
pH 8.5 @20°C (+0.1 mM Cu2+) = 0.6 hours
pH 8.5 @20°C (+0.1 mM EDTA) = 4 hours
DL-Dithiothreitol Preparation Products And Raw materials
Raw materials
Preparation Products
DL-Dithiothreitol Suppliers
- Tel
- 0512-68311833 13962195826
- Fax
- 0512-68417696
- wuhao@highfine.com
- Country
- China
- ProdList
- 499
- Advantage
- 75
- Tel
- 021-69955236-807 13918189704
- Fax
- 021-65211385
- chinaruji@chinaruji.com
- Country
- China
- ProdList
- 5176
- Advantage
- 55
- Tel
- 4009004166/18616739031 18616739031
- 3007523370@qq.com
- Country
- China
- ProdList
- 52693
- Advantage
- 58
- Tel
- 400-400-6206333 18521732826
- Fax
- 021-50323701
- market@aladdin-e.com
- Country
- China
- ProdList
- 25003
- Advantage
- 65
- Tel
- 400-910-1997 17560619001
- 3243204476@qq.com
- Country
- China
- ProdList
- 9959
- Advantage
- 58
- Tel
- 133-0845-3923 13319523173
- ivy@hnhbsj.com
- Country
- China
- ProdList
- 456
- Advantage
- 60
- Tel
- 0731-85561023 18973109536
- hengkangapi@hengkangapi.com
- Country
- China
- ProdList
- 394
- Advantage
- 58
- Tel
- 027-88878869 18162384755
- 969094702@qq.com
- Country
- China
- ProdList
- 59
- Advantage
- 58
- Tel
- 027-88878869 18162384755
- Fax
- 027-88878869
- xhkhu2014@163.com
- Country
- China
- ProdList
- 239
- Advantage
- 58
- Tel
- 0731-85526065 13308475853
- ivy@hnhbsj.com
- Country
- China
- ProdList
- 4552
- Advantage
- 62
- Tel
- 13681955282
- gyd@joiny-pharma.com
- Country
- China
- ProdList
- 208
- Advantage
- 58
- Tel
- 010-82848833 400-666-7788
- Fax
- 86-10-82849933
- jkinfo@jkchemical.com
- Country
- China
- ProdList
- 96815
- Advantage
- 76
- Tel
- 025-86918202 4000255188
- Fax
- 86-025-86918232
- sales@pharmablock.com
- Country
- China
- ProdList
- 4986
- Advantage
- 55
- Tel
- 021-61259108 18621169109
- Fax
- 86-21-61259102
- market03@meryer.com
- Country
- China
- ProdList
- 40228
- Advantage
- 62
- Tel
- 821-50328103-801 18930552037
- Fax
- 86-21-50328109
- 3bsc@sina.com
- Country
- China
- ProdList
- 15839
- Advantage
- 69
- Tel
- 400-6106006
- Fax
- 021-67582001/03/05
- saleschina@alfa-asia.com
- Country
- China
- ProdList
- 30123
- Advantage
- 84
- Tel
- 021-67121386
- Fax
- 021-67121385
- Sales-CN@TCIchemicals.com
- Country
- China
- ProdList
- 24529
- Advantage
- 81
- Tel
- 010-89508211 18501085097
- Fax
- 010-89508210
- sales.bj@hwrkchemical.com
- Country
- China
- ProdList
- 8418
- Advantage
- 55
- Tel
- 021-021-58432009 400-005-6266
- Fax
- 021-58436166
- sales8178@energy-chemical.com
- Country
- China
- ProdList
- 44688
- Advantage
- 61
- Tel
- 010-82967028 13552068683
- Fax
- +86-10-82967029
- 2355560935@qq.com
- Country
- China
- ProdList
- 12390
- Advantage
- 60
- Tel
- +86-025-81682922 +86-13515111588
- Fax
- 025-85860962
- sales@searchbio.com.cn
- Country
- China
- ProdList
- 135
- Advantage
- 68
- Tel
- 0523-86200218 18994667082
- Fax
- +86 523-86200613
- sale1_cmd@chemedir.com
- Country
- China
- ProdList
- 65
- Advantage
- 64
- Tel
- 13817811078
- Fax
- 86-021-50426522,50426273
- sales@jingyan-chemical.com
- Country
- China
- ProdList
- 9976
- Advantage
- 60
- Tel
- 400-6009262 16621234537
- Fax
- 021-64823266
- chenyj@titansci.com
- Country
- China
- ProdList
- 14103
- Advantage
- 59
- Tel
- 021-54306202 13764082696
- info@hanhongsci.com
- Country
- China
- ProdList
- 42958
- Advantage
- 64
- Tel
- 021-50135380
- shchemsky@sina.com
- Country
- China
- ProdList
- 32321
- Advantage
- 50
- Tel
- 025-58619198
- Fax
- 025-58619197
- sales@mbbio.com
- Country
- China
- ProdList
- 319
- Advantage
- 60
- Tel
- 010-82954736 13391601435
- sales@isomersyn.com
- Country
- China
- ProdList
- 3295
- Advantage
- 57
- Tel
- 4009903999 13355009207
- Fax
- 0539-6365991
- 3007715519@qq.com
- Country
- China
- ProdList
- 18738
- Advantage
- 57
- Tel
- Fax
- E-Mail Inquiry
- info@syntechem.com
- Country
- China
- ProdList
- 12984
- Advantage
- 57
- Tel
- 0315-8309571 15633399667
- Fax
- +86-315-7726572
- sales@moneidechem.com
- Country
- China
- ProdList
- 704
- Advantage
- 57
- Tel
- 400-1166-196 18981987031
- Fax
- 028-84555506 800101999
- cdhxsj@163.com
- Country
- China
- ProdList
- 11707
- Advantage
- 57
- Tel
- 400 638 7771
- sales@heowns.com
- Country
- China
- ProdList
- 14435
- Advantage
- 57
- Tel
- 86-21-63210123
- Fax
- 86-21-63290778 86-21-63218885
- sj_scrc@sinopharm.com
- Country
- China
- ProdList
- 9815
- Advantage
- 79
- Tel
- 0571-82693216
- Fax
- +86-571-82880190
- info@yuhaochemical.com
- Country
- China
- ProdList
- 9387
- Advantage
- 52
- Tel
- 021-021-021-67601398-809-809-809 15221380277
- Fax
- 021-57711696
- marketing_china@spectrumchemical.com
- Country
- China
- ProdList
- 9658
- Advantage
- 60
- Tel
- 027-59207852 13308628970
- Fax
- QQ3130921841
- buy@fortunachem.com
- Country
- China
- ProdList
- 2871
- Advantage
- 58
- Tel
- 0411-62910999 13889544652
- meilunui@163.com
- Country
- China
- ProdList
- 4727
- Advantage
- 58
- Tel
- +86 (25) 82263158
- Fax
- +86 (25) 82263168
- Country
- China
- ProdList
- 262
- Advantage
- 60
- Tel
- 021-61312847 13636370518
- Fax
- 021-55068248
- shyysw007@163.com
- Country
- China
- ProdList
- 4940
- Advantage
- 60
- Tel
- 4008-755-333 18080918076
- Fax
- 028-86757656
- 800078821@qq.com
- Country
- China
- ProdList
- 9718
- Advantage
- 55
- Tel
- 86-21-34053660
- Fax
- 86-21-34053661
- sale@labgogo.com
- Country
- China
- ProdList
- 9865
- Advantage
- 52
- Tel
- 0512-65617459 18915409046
- Fax
- 0512-65617459
- sales@sibian-chem.com
- Country
- China
- ProdList
- 1922
- Advantage
- 55
- Tel
- 800-810-5118
- Fax
- +86-10-84193589
- cnchemical@thermofisher.com
- Country
- China
- ProdList
- 17770
- Advantage
- 75
- Tel
- 010-56205725
- Fax
- 010-65763397
- waley188@sohu.com
- Country
- China
- ProdList
- 12335
- Advantage
- 58
- Tel
- 400-810-7969 010-59572699
- Fax
- 010-59572688
- ningzi.li@inno-chem.com.cn
- Country
- China
- ProdList
- 6134
- Advantage
- 55
- Tel
- +86-21-60341587
- Fax
- +86-21-61294319
- sales@topbiochem.com
- Country
- China
- ProdList
- 6175
- Advantage
- 58
- Tel
- 4000-027-021 |24 +86-13986109188 | +86-15623472865 | +81-08033611988
- Fax
- +86-27-87599188
- Country
- China
- ProdList
- 1493
- Advantage
- 55
- Tel
- +86 21 61551611
- Fax
- +86 21 50676805
- Country
- China
- ProdList
- 9891
- Advantage
- 58
- Tel
- 021-58950125
- Fax
- (86) 21-58955996
- info@chemexpress.com
- Country
- China
- ProdList
- 7552
- Advantage
- 61
View Lastest Price from DL-Dithiothreitol manufacturers
- Product
- DL-Dithiothreitol 3483-12-3
- Price
- US $100.00-75.00/kg
- Min. Order
- 1kg
- Purity
- 99%
- Supply Ability
- 5000Ton
- Release date
- 2024-08-12
- Product
- DL-1,4-Dithiothreitol 3483-12-3
- Price
- US $10.00/kg
- Min. Order
- 1kg
- Purity
- 99%
- Supply Ability
- 20ton
- Release date
- 2024-05-21
- Product
- DL-Dithiothreitol 3483-12-3
- Price
- US $1.00/g
- Min. Order
- 1g
- Purity
- 99%
- Supply Ability
- 200kg/month
- Release date
- 2021-06-10