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NHI 2

Product Name
NHI 2
CAS No.
1269802-97-2
Chemical Name
NHI 2
Synonyms
NHI 2;NHI-2 >=98% (HPLC);Methyl 1-hydroxy-6-phenyl-4-(trifluoromethyl)-1H-indole-2-carboxylate;1H-Indole-2-carboxylic acid, 1-hydroxy-6-phenyl-4-(trifluoromethyl)-, methyl ester
CBNumber
CB73146340
Molecular Formula
C17H12F3NO3
Formula Weight
335.28
MOL File
1269802-97-2.mol
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NHI 2 Property

Boiling point:
495.7±55.0 °C(Predicted)
Density 
1.36±0.1 g/cm3(Predicted)
storage temp. 
-20°
solubility 
Soluble in DMSO (up to 25 mg/ml).
form 
solid
pka
10+-.0.58(Predicted)
color 
Off-white
Stability:
Stable for 1 year from date of purchase as supplied. Solutions in DMSO or ethanol may be stored at -20° for up to 1 month.
InChI
1S/C17H12F3NO3/c1-24-16(22)15-9-12-13(17(18,19)20)7-11(8-14(12)21(15)23)10-5-3-2-4-6-10/h2-9,23H,1H3
InChIKey
YPPFWRWCZNXINO-UHFFFAOYSA-N
SMILES
ON(C(C(OC)=O)=C1)C2=C1C(C(F)(F)F)=CC(C3=CC=CC=C3)=C2
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Safety

WGK Germany 
WGK 3
Storage Class
11 - Combustible Solids
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Hazard and Precautionary Statements (GHS)

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
SML1463
Product name
NHI-2
Purity
≥98% (HPLC)
Packaging
5 mg
Price
$134
Updated
2025/07/31
Sigma-Aldrich
Product number
SML1463
Product name
NHI-2
Purity
≥98% (HPLC)
Packaging
25 mg
Price
$535
Updated
2025/07/31
Cayman Chemical
Product number
30201
Product name
NHI 2
Packaging
1mg
Price
$32
Updated
2024/03/01
Cayman Chemical
Product number
30201
Product name
NHI 2
Packaging
5mg
Price
$93
Updated
2024/03/01
Cayman Chemical
Product number
30201
Product name
NHI 2
Packaging
10mg
Price
$122
Updated
2024/03/01
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NHI 2 Chemical Properties,Usage,Production

Description

NHI 2 is an inhibitor of lactate dehydrogenase A (LDHA; IC50 = 14.7 μM). It is selective for LDHA over LDHB (IC50 = 55.8 μM). It inhibits lactate production in HeLa cells when used at concentrations of 100 and 200 μM. NHI 2 is cytotoxic to HeLa cells (IC50 = 33.4 μM).

Uses

NHI-2 is a lactate dehydrogenase A (LDHA) inhibitor with an IC50 of 14.7 μM. NHI-2 shows selective for LDHA over LDHB (IC50 = 55.8 μM). NHI-2 is an efficient anti-glycolytic agent. NHI-2 enhances apoptosis, induces cell cycle arrest at S/G2 phases. NHI-2 has a broad spectrum anti-proliferative activity in cancer cells. NHI-2 affects extracellular acidification rate and ATP production. NHI-2 suppress tumor growth in mice[1][2].

in vivo

NHI-2 (0.9 mg/kg; Intra-tumoral; once daily for 15 days) can suppress tumor volume when combined with immune checkpoint inhibitors (ICIs) in murine B78 melanoma tumor model[2].

Animal Model:C57BL/6 female mice (6-8 weeks) were intradermally implanted with B78 syngeneic melanoma cells[2]
Dosage:0.9 mg/kg
Administration:Intra-tumoral (IT) via percutaneous needle puncture; once daily for 15 days
Result:Suppressed tumor volume when combined with checkpoint inhibitors (ICIs).

storage

Store at +4°C

References

[1] GRANCHI C, CALVARESI E C, TUCCINARDI T, et al. Assessing the differential action on cancer cells of LDH-A inhibitors based on the N-hydroxyindole-2-carboxylate (NHI) and malonic (Mal) scaffolds†[J]. Organic & Biomolecular Chemistry, 2013, 38: 6588-6596. DOI:10.1039/c3ob40870a
[2] M MAFTOUH. Synergistic interaction of novel lactate dehydrogenase inhibitors with gemcitabine against pancreatic cancer cells in hypoxia[J]. British Journal of Cancer, 2013, 110 1: 172-182. DOI:10.1038/bjc.2013.681
[3] CARLOTTA GRANCHI. Discovery of N-Hydroxyindole-Based Inhibitors of Human Lactate Dehydrogenase Isoform A (LDH-A) as Starvation Agents against Cancer Cells[J]. Journal of Medicinal Chemistry, 2011, 54 6: 1599-1612. DOI:10.1021/jm101007q
[4] S J ALLISON. Identification of LDH-A as a therapeutic target for cancer cell killing via (i) p53/NAD(H)-dependent and (ii) p53-independent pathways[J]. Oncogenesis, 2014, 3 5: e102-e102. DOI:10.1038/oncsis.2014.16
[5] MICHAEL A LEA  Charles D  Yolanda Guzman. Inhibition of Growth by Combined Treatment with Inhibitors of Lactate Dehydrogenase and either Phenformin or Inhibitors of 6-Phosphofructo-2-kinase/Fructose-2,6-bisphosphatase 3.[J]. Anticancer research, 2016, 36 4: 1479-1488.

NHI 2 Preparation Products And Raw materials

Raw materials

Preparation Products

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NHI 2 Suppliers

China 19 United Kingdom 1 United States 7 Global 27
Guangzhou Isun Pharmaceutical Co., Ltd
Tel
020-39119399 18927568969
Fax
020-39119999
Email
isunpharm@qq.com
Country
China
ProdList
4720
Advantage
55
Shanghai ChuangYan Chemical Technology Co., Ltd.
Tel
021-11111111 11111111111
Fax
qq:546919421
Email
546919421@qq.com
Country
China
ProdList
3601
Advantage
55
Shanghai EFE Biological Technology Co., Ltd.
Tel
021-65675885 18964387627
Fax
021-65675885
Email
info@efebio.com
Country
China
ProdList
9803
Advantage
58
Shanghai YuanYe Biotechnology Co., Ltd.
Tel
021-61312847; 18021002903
Fax
QQ:3008007432
Email
3008007409@qq.com
Country
China
ProdList
71826
Advantage
60
Shanghai Hongye Biotechnology Co. Ltd
Tel
400-9205774
Email
sales@glpbio.cn
Country
China
ProdList
6777
Advantage
58
TargetMol Chemicals Inc.
Tel
+1-781-999-5354; +17819995354
Email
marketing@targetmol.com
Country
United States
ProdList
32470
Advantage
58
InvivoChem
Tel
+1-708-310-1919 +1-13798911105
Fax
708-557-7486
Email
sales@invivochem.cn
Country
United States
ProdList
6391
Advantage
58
ChemeGen(Shanghai) Biotechnology Co.,Ltd.
Tel
18818260767
Fax
QQ 3610331285
Email
sales@chemegen.com
Country
China
ProdList
11218
Advantage
58
Energy Chemical
Tel
021-58432009 400-005-6266
Fax
021-58436166
Email
marketing1@energy-chemical.com
Country
China
ProdList
44894
Advantage
58
Jinan Jiuli Biotechnology Co. , Ltd.
Tel
15865264761
Email
486064515@qq.com
Country
China
ProdList
4858
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58

1269802-97-2, NHI 2Related Search:

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  • NHI 2
  • Methyl 1-hydroxy-6-phenyl-4-(trifluoromethyl)-1H-indole-2-carboxylate
  • 1H-Indole-2-carboxylic acid, 1-hydroxy-6-phenyl-4-(trifluoromethyl)-, methyl ester
  • NHI-2 >=98% (HPLC)
  • 1269802-97-2