ChemicalBook > CAS DataBase List > 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride

1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride

Product Name
1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride
CAS No.
25952-53-8
Chemical Name
1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride
Synonyms
EDCI;EDC.HCl;EDAC;EDARAVONE;WSC;1-ETHYL-3-(3-DIMETHYLAMINOPROPYL) CARBODIIMIDE;WSCI;WSCD;1-Ethyl-3-(3-dimethyllaminopropyl)carbodiimide hydrochloride;EDC.HCI
CBNumber
CB7403031
Molecular Formula
C8H18ClN3
Formula Weight
191.7
MOL File
25952-53-8.mol
More
Less

1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride Property

Melting point:
110-115 °C(lit.)
Density 
0.877 g/mL at 20 °C(lit.)
vapor pressure 
0.002Pa at 20℃
refractive index 
n20/D 1.461
storage temp. 
-20°C
solubility 
H2O: soluble1 gm/10 ml, clear to very slightly hazy, colorless to very faintly yellow
form 
Crystalline Powder
color 
White to off-white
Water Solubility 
Soluble
Sensitive 
Hygroscopic
BRN 
5764110
Stability:
Stable, but sensitive to moisture. Incompatible with strong acids, strong oxidizing agents, moisture.
InChIKey
FPQQSJJWHUJYPU-UHFFFAOYSA-N
LogP
-2.98 at 25℃
CAS DataBase Reference
25952-53-8(CAS DataBase Reference)
EPA Substance Registry System
1,3-Propanediamine, N'-(ethylcarbonimidoyl)-N,N-dimethyl-, monohydrochloride (25952-53-8)
More
Less

Safety

Hazard Codes 
C,Xi
Risk Statements 
34-36/37/38-41-37/38-20/21/22
Safety Statements 
26-36/37/39-45-37/39-36
RIDADR 
UN 2735 8/PG 3
WGK Germany 
3
RTECS 
FF2200000
F 
1-3-10
Hazard Note 
Irritant
TSCA 
Y
HS Code 
29252000
Toxicity
LD50 intravenous in mouse: 56mg/kg
More
Less

Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Danger
Hazard statements

H302Harmful if swallowed

H311Toxic in contact with skin

H315Causes skin irritation

H317May cause an allergic skin reaction

H373May cause damage to organs through prolonged or repeated exposure

H410Very toxic to aquatic life with long lasting effects

Precautionary statements

P260Do not breathe dust/fume/gas/mist/vapours/spray.

P273Avoid release to the environment.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P301+P312IF SWALLOWED: call a POISON CENTER or doctor/physician IF you feel unwell.

P314Get medical advice/attention if you feel unwell.

More
Less

N-Bromosuccinimide Price

Sigma-Aldrich
Product number
E6383
Product name
N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride
Purity
crystalline
Packaging
25G
Price
$570
Updated
2024/03/01
Sigma-Aldrich
Product number
8.00907
Product name
N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride
Purity
forsynthesis
Packaging
500mg
Price
$43.9
Updated
2024/03/01
Sigma-Aldrich
Product number
8.00907
Product name
N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride
Purity
forsynthesis
Packaging
1g
Price
$56.6
Updated
2024/03/01
Sigma-Aldrich
Product number
8.00907
Product name
N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride
Purity
forsynthesis
Packaging
5G
Price
$165
Updated
2024/03/01
Sigma-Aldrich
Product number
8.00907
Product name
N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride
Purity
forsynthesis
Packaging
25g
Price
$343
Updated
2024/03/01
More
Less

1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride Chemical Properties,Usage,Production

Chemical Properties

EDC-HCl, or 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, is a white crystalline powder that easily deliquescent and can dissolve in both water and ethanol. It is commonly used as an activating reagent in amide synthesis to activate carboxyl groups. It can also activate phosphate groups, cross-link proteins and nucleic acids, and produce immunocouplers. EDC-HCl is commonly applied in the pH range of 4.0-6.0 and is often used in conjunction with N-Hydroxysuccinimide (NHS) or N-Hydroxysulfosuccinimide sodium salt to improve coupling efficiency.

Uses

1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride is used as a carboxyl activating agent. It plays a vital role for immobilization of large biomolecules in association with N-hydroxysuccinimide. It is also used in the acylation of phosphoranes.

Application

1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride is water-soluble carbodiimide, widely used for peptide coupling.
N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride has been used for the formation of FND (fluorescent nanodiamonds)-transferrin bioconjugates.
It has been used for crosslinking polyethylenimine to gold particles.
It has been used as a carbodiimide linkage agent for coating of carboxylated polystyrene beads with biotinylated BSA (bovine serum albumin).

General Description

EDC hydrochloride is a water-soluble derivative of carbodiimide useful for conjugating haptens to proteins and polypeptides. Used to modify NMDA receptors and as a condensing agent in peptide synthesis. The major advantage of EDAC coupling is the easy removal of excess reagent and the corresponding urea by washing with dilute acid or water. Carbodiimides catalyze the formation of amide bonds, carboxylic acids, and amines by activating the carboxylate to form an O-acylurea. This intermediate can be attacked by an amine directly to form an amide. EDAC is released as a soluble urea derivative.

Flammability and Explosibility

Flammable

Biochem/physiol Actions

1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride(25952-53-8) is a water soluble condensing reagent. EDAC is generally utilized as a carboxyl activating agent for amide bonding with primary amines. Additionally, it reacts with phosphate groups. It has been utilized in peptide synthesis, crosslinking proteins to nucleic acids as well as preparation of immunoconjugates.

Mechanism of action

Carboxyl group activation with EDC. xHCl proceeds similarly to other carbodiimide couplings. The reaction of a carboxylic acid and the carbodiimide forms an O-acylisourea intermediate. The O-acylisourea is a highly reactive species that readily reacts with amines, peptide coupling additives, or reducing agents. EDC. HCl is transformed into the corresponding urea during coupling reactions. It has the advantage over DCU in that it can be removed from the reaction mixture by extraction or be washed out from solid phase synthesis applications. However, the O-acylisourea can rearrange irreversibly to an N-acyl urea and racemise the α-carbon of the amino acid via the formation of an oxazol-4(5H)-one [azlactone]. N-Acylurea formation and racemisation may be reduced by using intermediate nucleophiles, which convert the O-acylurea to an activated ester containing the nucleophile. The mechanism of amide bond formation applies both to EDC and EDC.HCl. The differences are that the free base can be applied as a bifunctional reagent: a coupling agent (carbodiimide) and a base (dimethylamino). At the same time, the HCl salt can form an O-acylisourea cyclic intermediate.

Synthesis

1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride can be used for the synthesis of amides.It is used as a coupling agent in the synthesis of esters from carboxylic acids using dimethylaminopyridine as the catalyst.
Preparation method of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride(CN104193654A)

Purification Methods

It is an excellent H2O-soluble peptide coupling reagent. It is purified by dissolving (ca 1g) in CH2Cl2 (10mL) at room temperature and then add dry Et2O (~110mL) dropwise and the crystals that separate are collected, washed with dry Et2O, recrystallised from CH2Cl2/Et2O and dried in a vacuum over P2O5. It is important to work in a dry atmosphere or work rapidly and then dry the solid as soon as possible. The material is moderately hygroscopic, but once it becomes wet it reacts slowly with H2O. Store it away from moisture at -20o to slow down the hydrolysis process. The free base has b 47-48o/0.27mm, 53-54o/0.6mm, n 1.4582. The methiodide is recrystallised from CHCl3/EtOAc, the crystals are filtered off, washed with dry Et2O, recrystallised from CHCl3/Et2O, and dried in vacuo over P2O5, m 93-95o, 94-95o. [Sheehan et al. J Am Chem Soc 87 2492 1965, Sheehan & Cruickshank Org Synth Coll Vol V 555 1973.] § A polymer bound version is commercially available.

1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride Preparation Products And Raw materials

Raw materials

Preparation Products

2-AMINO-5-BROMOBENZAMIDE 6-BROMO-4-CHLORO-2-METHYL-QUINAZOLINE 6-bromo-2-methylquinazolin-4(3H)-one
More
Less

1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride Suppliers

Americas 1 Belgium 3 Canada 7 China 679 Europe 6 France 4 Germany 15 Hungary 1 India 86 Ireland 1 Israel 1 Japan 20 Poland 1 Russia 1 Slovakia 1 South Korea 3 Switzerland 7 United Kingdom 27 United States 80 Global 944
Pure Chemistry Scientific Inc.
Tel
001-857-928-2050 or 1-888-588-9418
Fax
001-617-206-9595
Email
sales@chemreagents.com
Country
United States
ProdList
10186
Advantage
62
ApexBio Technology LLC
Tel
+1-832-696-8203
Fax
+1-832-641-3177
Email
info@apexbt.com
Country
United States
ProdList
477
Advantage
60
HBCChem, Inc.
Tel
+1-510-219-6317
Fax
+1-650-486-1361
Email
sales@hbcchem.com
Country
United States
ProdList
10648
Advantage
60
CovaChem, LLC.
Tel
+1815.714.8421 (direct)
Fax
+1815.315.1272
Email
tonynooner@covachem.com
Country
United States
ProdList
89
Advantage
58
BOC Sciences
Tel
16314854226; +16314854226
Email
inquiry@bocsci.com
Country
United States
ProdList
19741
Advantage
58
TargetMol Chemicals Inc.
Tel
+1-781-999-5354 +1-00000000000
Email
marketing@targetmol.com
Country
United States
ProdList
32161
Advantage
58
Cato Research Chemicals Inc.
Tel
020-81215950 4000-868-328
Email
customer@uwalab.com
Country
United States
ProdList
10404
Advantage
58
Alchem Pharmtech,Inc.
Tel
8485655694
Email
sales@alchempharmtech.com
Country
United States
ProdList
63687
Advantage
58
AB PharmaTech,LLC
Tel
323-480-4688
Fax
323-480-4688
Email
sales@acrospharmatech.com
Country
United States
ProdList
989
Advantage
55
Protheragen-ING
Tel
+16313385890
Email
info@protheragen-ing.com
Country
United States
ProdList
3868
Advantage
58
Aladdin Scientific
Tel
+1-+1(833)-552-7181
Email
sales@aladdinsci.com
Country
United States
ProdList
57505
Advantage
58
Aceschem Inc.
Tel
+1-817863-6948 +1-（817）863-6948
Email
sales@aceschem.com
Country
United States
ProdList
19632
Advantage
58
Alfa Chemistry
Tel
Email
info@alfa-chemistry.com
Country
United States
ProdList
2344
Advantage
58
Aston Chemical
Tel
13000000000
Email
sales@astonchem.com
Country
United States
ProdList
1634
Advantage
58
Synthonix Inc
Tel
--
Fax
--
Email
info@synthonix.com
Country
United States
ProdList
6872
Advantage
58
ApexBio Technology
Tel
--
Fax
--
Email
sales@apexbt.com
Country
United States
ProdList
6251
Advantage
58
CarboMer, Inc.
Tel
--
Fax
--
Email
sales@carbomer.com
Country
United States
ProdList
4026
Advantage
67
Abcam
Tel
--
Fax
--
Email
us.orders@abcam.com
Country
United States
ProdList
6000
Advantage
50
TCI America, Inc. (Tokyo Chemical Industry Co., Ltd.)
Tel
--
Fax
--
Email
.Crew@tcichemicals.com
Country
United States
ProdList
3164
Advantage
58
Advanced ChemTech (a division of CreoSalus Inc.)
Tel
--
Fax
--
Email
info@advancedchemtech.com
Country
United States
ProdList
177
Advantage
58
Kaneka America LLC (part of Kaneka Corporation Japan)
Tel
--
Fax
--
Email
alexis.mercado@kaneka.com
Country
United States
ProdList
51
Advantage
58
MP Biomedicals, LLC. (Division of Valiant FC Co., Ltd.)
Tel
--
Fax
--
Email
serv@mpbio.com
Country
United States
ProdList
1854
Advantage
58
Redox Scientific
Tel
--
Fax
--
Email
info@redoxsci.com
Country
United States
ProdList
2380
Advantage
58
Chemphtec Corporation
Tel
--
Fax
--
Email
fo@chemphtec.com
Country
United States
ProdList
2181
Advantage
58
Alichem Inc.
Tel
--
Fax
--
Email
sales@alichem.com
Country
United States
ProdList
6167
Advantage
58
MilliporeSigma (Sigma-Aldrich Corp.)
Tel
--
Fax
--
Email
@sial.com
Country
United States
ProdList
5414
Advantage
58
Fluorochem Ltd.
Tel
--
Fax
--
Email
enquiries@fluorochem.co.uk
Country
United States
ProdList
4583
Advantage
58
Pfaltz & Bauer, Inc.
Tel
--
Fax
--
Email
sales@pfaltzandbauer.com
Country
United States
ProdList
6828
Advantage
72
VWR International, LLC.
Tel
--
Fax
--
Email
@sargentwelch.com
Country
United States
ProdList
6262
Advantage
58
Charkit Chemical Corporation
Tel
--
Fax
--
Email
sales@charkit.com
Country
United States
ProdList
2993
Advantage
65
OChem Incorporation
Tel
--
Fax
--
Email
sales@ocheminc.com
Country
United States
ProdList
6501
Advantage
60
Chem-Impex International, Inc. (CII)
Tel
--
Fax
--
Email
Customer@chemimpex.com
Country
United States
ProdList
893
Advantage
58
Spectrum Chemical Mfg. Corp.
Tel
--
Fax
--
Email
marketing@spectrumchemical.com
Country
United States
ProdList
3113
Advantage
60
Combi-Blocks Inc.
Tel
--
Fax
--
Email
sales@combi-blocks.com
Country
United States
ProdList
6618
Advantage
69
SynQuest Laboratories, Inc.
Tel
--
Fax
--
Email
info@synquestlabs.com
Country
United States
ProdList
6871
Advantage
62
Riedel-de Haen AG
Tel
--
Fax
--
Country
United States
ProdList
6773
Advantage
87
TCI America
Tel
--
Fax
--
Email
sales@tciamerica.com
Country
United States
ProdList
6909
Advantage
75
Frontier Scientific, Inc.
Tel
--
Fax
--
Email
sales@frontiersci.com
Country
United States
ProdList
6222
Advantage
86
Matrix Scientific
Tel
--
Fax
--
Email
sales@matrixscientific.com
Country
United States
ProdList
6632
Advantage
80
Richman Chemical Inc.
Tel
--
Fax
--
Email
kac@richmanchemical.com
Country
United States
ProdList
692
Advantage
67
AppliChem, LLC
Tel
--
Fax
--
Email
sales@applichem.com
Country
United States
ProdList
169
Advantage
50
HBCChem, Inc.
Tel
--
Fax
--
Email
Sales@hbcchem-inc.com
Country
United States
ProdList
887
Advantage
50
Advanced Asymmetrics, Inc.
Tel
--
Fax
--
Email
sales@advancedasymmetrics.com
Country
United States
ProdList
521
Advantage
42
AAPPTEC
Tel
--
Fax
--
Email
info@aapptec.com
Country
United States
ProdList
533
Advantage
0
Anvia Chemicals, LLC
Tel
--
Fax
--
Email
sales@anviachem.com
Country
United States
ProdList
3933
Advantage
0
ProteoChem Inc.
Tel
--
Fax
--
Email
info@proteochem.com
Country
United States
ProdList
74
Advantage
38
HBCChem Inc.
Tel
--
Fax
--
Email
sales@hbcchem-inc.com
Country
United States
ProdList
4569
Advantage
30
Waterstone Technology, LLC
Tel
--
Fax
--
Email
sales@waterstonetech.com
Country
United States
ProdList
6786
Advantage
30
AlliChem, LLC
Tel
--
Fax
--
Email
sales@allichemllc.com
Country
United States
ProdList
6516
Advantage
60
Oakwood Products, Inc.
Tel
--
Fax
--
Email
sales@oakwoodchemical.com
Country
United States
ProdList
6193
Advantage
74
More
Less

View Lastest Price from 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride manufacturers

Wuhan Circle Star Chem-medical Technology Co.,Ltd
Product
1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride 25952-53-8
Price
US $0.00-0.00/kg
Min. Order
1kg
Purity
98
Supply Ability
1000
Release date
2024-12-26
Jinan Finer Chemical Co., Ltd
Product
1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride 25952-53-8
Price
US $0.00/kg
Min. Order
100g
Purity
99%
Supply Ability
100mt
Release date
2019-03-15
Hebei Zhuanglai Chemical Trading Co Ltd
Product
1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride 25952-53-8
Price
US $10.00/kg
Min. Order
1kg
Purity
99%
Supply Ability
20 ton
Release date
2024-11-18

25952-53-8, 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochlorideRelated Search:

O-Phthalimide Ethyl acrylate Dimethyl sulfoxide Ethylparaben N,N-Diisopropylethylamine Dimethylamine hydrochloride Cyanamide N,N-Dimethylacetamide Triethylamine hydrochloride Methoxyammonium chloride N,N-Dimethylformamide N,N-Dimethylallylamine Ethanol Poly(dimethylsiloxane) Topotecan hydrochloride 2-Ethylphenylhydrazine hydrochloride Ethyl cellulose Amines, N-tallow alkyltrimethylenedi-

  • (3-dimethylaminopropyl)ethyl-carbodiimidmonohydrochloride
  • 3-propanediamine,n’-(ethylcarbonimidoyl)-n,n-dimethyl-monohydrochloride
  • 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydroc
  • AT test ,only COA
  • EDC.HCI
  • N'-(Ethylkohlenstoffimidoyl)-N,N-dimethylpropan-1,3-diaminmonohydrochlorid
  • Avanafil Impurity 40
  • Diquafosol Impurity m
  • carbodiimide HydrochL
  • oride (EDCI)
  • 1-ethyl-(3-dimethylaminopropyl)carbonyldiimide hydrochloride
  • edap
  • edc(reagent)
  • WSCD
  • WSCI
  • WATER-SOLUBLE CARBODIIMIDE
  • 1-Ethyl-3-(3-dimethyllaminopropyl)carbodiimide hydrochloride
  • 1-ETHYL-3-(3-DIMETHYLAMINOPROPYL)- &
  • N-(DIMETHYLAMINOPROPYL)-N'-ETHYLCARBO- DI IMIDE HCL
  • 1-(3-(DIMETHYLAMINO)PROPYL)-3-ETHYL-CARBODIIMIDE HYDROCHLORIDE, 98+%
  • 1-(3-Dimethylaminopropyl)-3-ethyl-carbodiimide hydrochloride (EDC)
  • 1-ETHYL-3-(3-DIMETHYLAMINOPROPYL) CARBODIIMIDE
  • EDAC
  • EDCI
  • EDC (WSC-HCl)
  • WS.HCl
  • 1-ethyl-3-(3-Dimethylaminopropyl) carbodiimide HCl(EDC HCl)
  • eDC [1-(3-Dimethylaminopropyl)-3-ethyl-carbodiimide]
  • eDC HCl [1-ethyl-3-(3-Dimethylaminopropyl) carbodiimide HCl]
  • 1-ETHYL-3-(3-DIMETHYLAMINOPROPYL)-*CARBO DIIMIDE HYD
  • 1-ETHYL-3-(3-DIMETHYLAMINOPROPYL)*CARBOD IIMIDE HYDR
  • EDAC hydrochloride, EDC hydrochloride
  • 1-Ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride, anhydrous
  • 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide Hydrochloride [Coupling Agent for Peptides Synthesis]
  • N-(3-Dimethylaminopropyl)-Nμ-ethylcarbodiimide hydrochloride (EDC) 98%
  • 3-(Ethyliminomethylideneamino)-N,N-dimethylpropan-1-amine hydrochloride
  • Carbodiimide, (3-dimethylaminopropyl)ethyl-, monohydrochloride
  • N-(3-Dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride ,99%
  • Edccl
  • 1-[3-(Dimethylamino)propyl]-3-ethylcarbodiimide·hydrochloric acid
  • 1-Ethyl-3-[3-(dimethylamino)propyl]carbodiimide·hydrochloric acid
  • N-[3-(Dimethylamino)propyl]-N'-ethylcarbodiimide·hydrochloric acid
  • N-Ethyl-N'-[3-(dimethylamino)propyl]carbodiimide·hydrochloric acid
  • 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochlorid
  • EDAC hydrochloride (N-(3-Dimethylaminopropyl)-N'-ethylcarbodiimi
  • 1-(3-DiMethylaMinopropyl)-3-ethylcarbodiiMide hydrochloride CAS: 25952-53-8
  • (3-DiMethylaMino-propyl)-ethyl-carbodiiMide Hydrochloride, EDC HCl, EDAC, EDCI, Water Soluble CarbodiiMide.
  • 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide Hydrochloride EDC EDAC EDCI
  • EDAC · HCl, N-(3-DiMethylaMinopropyl)-N'-ethylcarbodiiMide · HCl, Water-soluble CarbodiiMide, WSC, EDC · HCl
  • EDCHC1/EDACHC1
  • N-(3-DIMETHYLAMINOPROPYL)-N'-ETHYLCARBOD
  • EDC.HCL 1-Ethyl-3-(3-diMethyllaMinopropyl)carbodiiMide hydrochloride
  • -(3-DiMethylaMinopropyl)-3-ethylcarbodiiMide hydrochloride
  • 1-(3-DiMethylaMinopropyl)-3-ethylcabodiiMide HCl
  • 3-(ethyliMinoMethyleneaMino)-N,N-diMethylpropan-1-aMine
  • EDC hydrochloride, N,N-Dimethyl-3-{[(ethylimino)methylidene]amino}propylamine hydrochloride
  • 1-[3-(DIMETHYLAMINO)PROPYL]-3-ETHYLCARBODIIMIDE(EDC.HCL)
  • EDC.HCL 1-ETHYL-3-(3-DIMETHYLLAMINOPROPYL)CARBODIIMIDE HYDROCHLORIDE / EDAC / EDCI