Tris(2-carboxyethyl)phosphine hydrochloride
- Product Name
- Tris(2-carboxyethyl)phosphine hydrochloride
- CAS No.
- 51805-45-9
- Chemical Name
- Tris(2-carboxyethyl)phosphine hydrochloride
- Synonyms
- TCEP HCL;TCEP Solution;Tris(2-carboxyethyl)phosphine hydrochloride, 98% 1GR;TCEP*HCL;TCEP-HCI;0.5M TCEP;500 MM TCEP;TCEP, HYDROCHLORIDE;)phosphine hydrochL;TCEP-HCl, OMniPur(R)
- CBNumber
- CB8263816
- Molecular Formula
- C9H15O6P.ClH
- Formula Weight
- 286.65
- MOL File
- 51805-45-9.mol
Tris(2-carboxyethyl)phosphine hydrochloride Property
- Melting point:
- 177 °C
- Density
- 1.041 g/mL at 25 °C
- refractive index
- n20/D 1.367
- storage temp.
- 2-8°C
- solubility
- H2O: 50 mg/mL
- form
- powder
- pka
- 7.66(at 25℃)
- color
- white
- PH
- 7.0(aqueous solution; pH was adjusted with ammonium hydroxide)
- Water Solubility
- Soluble in water and methanol.
- Sensitive
- Moisture Sensitive
- λmax
- λ: 260 nm Amax: 0.05
λ: 280 nm Amax: 0.03 - Merck
- 14,9085
- BRN
- 3724376
- Stability:
- Stable for 1 year from date of purchase as supplied. Solutions in distilled water may be stored at -20°C for up to 1 month.
- InChIKey
- PBVAJRFEEOIAGW-UHFFFAOYSA-N
- CAS DataBase Reference
- 51805-45-9(CAS DataBase Reference)
Safety
- Hazard Codes
- C,Xi
- Risk Statements
- 36/38-36/37/38-34
- Safety Statements
- 26-37/39-36/37/39-27-45
- RIDADR
- UN 3261 8/PG 2
- WGK Germany
- 2
- F
- 10
- TSCA
- No
- HazardClass
- 8
- PackingGroup
- III
- HS Code
- 29319090
Hazard and Precautionary Statements (GHS)
- Symbol(GHS)
-
- Signal word
- Danger
- Hazard statements
-
H314Causes severe skin burns and eye damage
- Precautionary statements
-
P260Do not breathe dust/fume/gas/mist/vapours/spray.
P280Wear protective gloves/protective clothing/eye protection/face protection.
P301+P330+P331IF SWALLOWED: Rinse mouth. Do NOT induce vomiting.
P303+P361+P353IF ON SKIN (or hair): Remove/Take off Immediately all contaminated clothing. Rinse SKIN with water/shower.
P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.
N-Bromosuccinimide Price
- Product number
- 68957
- Product name
- Tris(2-carboxyethyl)phosphine hydrochloride
- Purity
- BioUltra, for electrophoresis, SDS-PAGE tested
- Packaging
- 10g
- Price
- $1110
- Updated
- 2024/03/01
- Product number
- 646547
- Product name
- Tris(2-carboxyethyl)phosphine hydrochloride solution
- Purity
- 0.5 M, pH 7.0(aqueous solution; pH was adjusted with ammonium hydroxide)
- Packaging
- 10x1ml
- Price
- $132
- Updated
- 2024/03/01
- Product number
- 580567
- Product name
- TCEP,Hydrochloride,ReagentGrade-CAS51805-45-9-Calbiochem
- Packaging
- 5G
- Price
- $338
- Updated
- 2024/03/01
- Product number
- 580560
- Product name
- TCEP, Hydrochloride - CAS 51805-45-9 - Calbiochem
- Purity
- A water-soluble and odorless disulfide-reducing agent that is more stable and effective than DTT.
- Packaging
- 1G
- Price
- $122
- Updated
- 2022/05/15
- Product number
- T1656
- Product name
- Tris(2-carboxyethyl)phosphine Hydrochloride
- Purity
- >98.0%(T)
- Packaging
- 1g
- Price
- $53
- Updated
- 2024/03/01
Tris(2-carboxyethyl)phosphine hydrochloride Chemical Properties,Usage,Production
Description
TCEP HCl (51805-45-9) is a reagent for selective reduction of disulfides in aqueous solutions1-3?with distinct advantages over DTT which are application specific3. For example, spin labels are more stable with TCEP than DTT, Ni2+?(leached from affinity columns) causes rapid oxidation of DTT with no effect on TCEP and for long term storage of proteins TCEP is more stable than DTT with no metal chelator (EGTA) present but DTT is more stable in the presence of metal chelators.3
Chemical Properties
White Crystalline Solid
Uses
TCEP (Tris(2-carboxyethyl)phosphine hydrochloride) is an alternative which is not pungent, more stable and works even at low pH. It is more selective, i.e. do not reduce metals and buried disulfides.
Uses
Reducing agent, specific for disulfidesTris(2-carboxyethyl)phosphine hydrochloride is widely used as a reducing agent to reduce disulfide bonds in proteins and trans-4,5-dihydroxy-1,2-dithiane. It is involved in the reduction of sulfoxides, sulfonyl chlorides, N-oxides, and azides. It plays an important role in the tissue homogenization process for RNA isolation. It acts as a chelating agent and forms complexes with various heavy metal ions such as Zn(II), Cd(II), Pb(II), and Ni(II). Furthermore, it is useful in the labeling of cysteine residues with maleimides.
Uses
Biochemical tool for selective reduction of disulfide bridges at low pH; reductant for redox assays.
Preparation
The preparation of Tris(2-carboxyethyl)phosphine hydrochloride is as follows:Trimethyl 3,3 ', 3 "-phosphine triphenylpropionate (92.5g, 0.28mol) prepared as described above was added to a 250 ml reaction flask under mechanical stirring conditions and a molar ratio of 12mol/(120ml, 1.44mol), the mixture was heated to 90°C, the solid gradually dissolved, continue to heat the reaction 4h, stirring and cooling with ice water, precipitation of white solid, filter, filter cake with distilled water to get white (2-carboxyethyl) phosphine hydrochloride, dried, weighing 61.3g, yield 82.4%
General Description
Tris (2-carboxyethyl) phosphine (TCEP) is very effective in cleaving disulfide bonds in aqueous solution. It dissolves in water and is odorless, unlike other trialkylphosphines (tributylphosphine). It is also less toxic than 2-mercaptoethanol. These advantages make it better than the other reducing agents.
Reactivity Profile
Nucleophilic Substitution by the Phosphorus Atom of TCEP. 1. The phosphorus atom attacks one sulfur atom along the S-S bond. 2. A thioalkoxyphosphonium cation and a sulfhydryl anion are formed. 3. A rapid hydrolysis releases the second sulfhydryl molecule and the phosphine oxide. There are two main steps in the breaking of disulfide bridges and forming a free sulfhydryl using TCEP: cleavage of the S-S bond; oxidation of the phosphine and release of sulfhydryl.
Biochem/physiol Actions
Tris(2-carboxyethyl)phosphine hydrochloride solution reduces the disulfide bonds and leaves other functional groups intact in proteins.
storage
Following reconstitution, aliquot and freeze (-20°C). TCEP retains stability when stored at an acidic or alkaline pH; avoid storage at pH 7.0-8.0. Also avoid exposure to high concentrations of phosphate ions (>150 mM). Stock solutions are stable for up to 3 months at -20°C.
References
1) Fischer?et al.?(1993),?In situ reduction suitable for matrix-assisted laser desorption/ionization and liquid secondary ionization using tris(2-carboxyethyl)phosphine; Rapid Commun. Mass Spectrom.,?7?225 2) Kirley?et al.?(1989),?Reduction and fluorescent labeling of cyst(e)ine-containing proteins for subsequent structural analysis; Anal. Biochem.,?180?231 3) Getz?et al. (1999),?A comparison between the sulfhydryl reductants tris(2-carboxyethyl)phosphine and dithiothreitol for use in protein biochemistry; Anal. Biochem.,?273?73
Tris(2-carboxyethyl)phosphine hydrochloride Preparation Products And Raw materials
Raw materials
Preparation Products
Tris(2-carboxyethyl)phosphine hydrochloride Suppliers
- Tel
- 0512-68311833 13962195826
- Fax
- 0512-68417696
- wuhao@highfine.com
- Country
- China
- ProdList
- 498
- Advantage
- 75
- Tel
- 0512-65045967 15365387483
- Fax
- 15365387483
- sales@haihengpharm.com
- Country
- China
- ProdList
- 94
- Advantage
- 55
- Tel
- 021-67121386
- Fax
- 021-67121385
- Sales-CN@TCIchemicals.com
- Country
- China
- ProdList
- 24539
- Advantage
- 81
- Tel
- 027-88878869 18162384755
- Fax
- 027-88878869
- xhkhu2014@163.com
- Country
- China
- ProdList
- 238
- Advantage
- 58
- Tel
- 027-88878869 18162384755
- 969094702@qq.com
- Country
- China
- ProdList
- 59
- Advantage
- 58
- Tel
- 15252131412
- 5954981@qq.com
- Country
- China
- ProdList
- 2964
- Advantage
- 58
- Tel
- 0512-87182056 13451648594
- Fax
- 0512-87182056
- sales@yacoo.com.cn
- Country
- China
- ProdList
- 5000
- Advantage
- 58
- Tel
- 15866604075 15866604075
- 15195457346m@sina.cn
- Country
- China
- ProdList
- 513
- Advantage
- 58
- Tel
- 19137644811
- shisx1211@163.com
- Country
- China
- ProdList
- 497
- Advantage
- 58
- Tel
- 025-87138562 18951783565
- sales@popebiotech.com
- Country
- China
- ProdList
- 1019
- Advantage
- 58
- Tel
- 010-82848833 400-666-7788
- Fax
- 86-10-82849933
- jkinfo@jkchemical.com
- Country
- China
- ProdList
- 96815
- Advantage
- 76
- Tel
- 400-0255188 4000255188
- Fax
- 86-025-86918232
- sales@pharmablock.com
- Country
- China
- ProdList
- 4986
- Advantage
- 55
- Tel
- 021-61259108 18621169109
- Fax
- 86-21-61259102
- market03@meryer.com
- Country
- China
- ProdList
- 40240
- Advantage
- 62
- Tel
- 010-88425576
- Fax
- (+86)-10-88425546
- sales@dhltchem.com
- Country
- China
- ProdList
- 793
- Advantage
- 59
- Tel
- 400-6106006
- Fax
- 021-67582001/03/05
- saleschina@alfa-asia.com
- Country
- China
- ProdList
- 30132
- Advantage
- 84
- Tel
- 0757-86329057 18501085097
- Fax
- 010-89508210
- sales3.gd@hwrkchemical.com
- Country
- China
- ProdList
- 7639
- Advantage
- 55
- Tel
- 021-021-58432009 400-005-6266
- Fax
- 021-58436166
- sales8178@energy-chemical.com
- Country
- China
- ProdList
- 44751
- Advantage
- 61
- Tel
- 86-027-67849912
- Fax
- 86-027-87531808
- sales@chemwish.com
- Country
- China
- ProdList
- 35906
- Advantage
- 56
- Tel
- 21-61263452 13641803416
- Fax
- 86-21-61263399
- ymbetter@glbiochem.com
- Country
- China
- ProdList
- 9981
- Advantage
- 64
- Tel
- +86 (0) 571 85 58 67 18
- Fax
- 0086-571-85864795
- sales@capotchem.com
- Country
- China
- ProdList
- 18217
- Advantage
- 66
- Tel
- +86-21-54098501
- Fax
- +86-21-54096319
- sales@sinch.com.cn
- Country
- China
- ProdList
- 11605
- Advantage
- 64
- Tel
- 0573-85285100 18627885956
- Fax
- 0573-85285100
- isenchem@163.com
- Country
- China
- ProdList
- 9551
- Advantage
- 66
- Tel
- 400-6009262 16621234537
- Fax
- 021-64823266
- zhangsn@titansci.com
- Country
- China
- ProdList
- 14113
- Advantage
- 59
- Tel
- 021-50795510 4000665055
- Fax
- 021-50795055
- sales@accelachem.com
- Country
- China
- ProdList
- 11660
- Advantage
- 64
- Tel
- Country
- China
- ProdList
- 42982
- Advantage
- 64
- Tel
- 021-50135380
- shchemsky@sina.com
- Country
- China
- ProdList
- 32344
- Advantage
- 50
- Tel
- 13355009207 13355009207
- Fax
- 0539-6365991
- 3007715519@qq.com
- Country
- China
- ProdList
- 18739
- Advantage
- 57
- Tel
- Fax
- E-Mail Inquiry
- info@syntechem.com
- Country
- China
- ProdList
- 12990
- Advantage
- 57
- Tel
- 400-1166-196 18981987031
- Fax
- 028-84555506 800101999
- cdhxsj@163.com
- Country
- China
- ProdList
- 11726
- Advantage
- 57
- Tel
- 400 638 7771
- sales@heowns.com
- Country
- China
- ProdList
- 14443
- Advantage
- 57
- Tel
- +8613162137806 18575662672
- Fax
- 021 51613951
- mzeng@3wpharm.com
- Country
- China
- ProdList
- 9746
- Advantage
- 57
- Tel
- 0731-85526065 13308475853
- ivy@hnhbsj.com
- Country
- China
- ProdList
- 4550
- Advantage
- 62
- Tel
- Please Send Email 18652950785
- Fax
- 025-87193546
- chemweiao@163.com
- Country
- China
- ProdList
- 4388
- Advantage
- 65
- Tel
- 021-021-021-67601398-809-809-809 15221380277
- Fax
- 021-57711696
- marketing_china@spectrumchemical.com
- Country
- China
- ProdList
- 9664
- Advantage
- 60
- Tel
- 027-027-59207852 13308628970
- Fax
- QQ3130921841
- buy@fortunachem.com
- Country
- China
- ProdList
- 2893
- Advantage
- 58
- Tel
- 0411-62910999 13889544652
- sales@meilune.com
- Country
- China
- ProdList
- 4654
- Advantage
- 58
- Tel
- 021-61312847 13636370518
- Fax
- 021-55068248
- shyysw007@163.com
- Country
- China
- ProdList
- 4941
- Advantage
- 60
- Tel
- 4008-755-333 18080918076
- Fax
- 028-86757656
- 800078821@qq.com
- Country
- China
- ProdList
- 9725
- Advantage
- 55
- Tel
- 86-21-34053660
- Fax
- 86-21-34053661
- sale@labgogo.com
- Country
- China
- ProdList
- 9872
- Advantage
- 52
- Tel
- 800-810-5118
- Fax
- +86-10-84193589
- cnchemical@thermofisher.com
- Country
- China
- ProdList
- 17779
- Advantage
- 75
- Tel
- 400-810-7969 010-59572699
- Fax
- 010-59572688
- ningzi.li@inno-chem.com.cn
- Country
- China
- ProdList
- 6139
- Advantage
- 55
- Tel
- 4009209199
- Fax
- 86-021-52271987
- sales@9dingchem.com
- Country
- China
- ProdList
- 22519
- Advantage
- 55
- Tel
- 18521735133 18521732826;
- Fax
- 021-50323701
- market@aladdin-e.com
- Country
- China
- ProdList
- 25014
- Advantage
- 65
- Tel
- 021-61552785
- Fax
- 021-55660885
- sales@shshiji.com
- Country
- China
- ProdList
- 9552
- Advantage
- 55
- Tel
- +86-21-65211385-8001 36031160
- Fax
- +86-21-65211385-8004
- sales@shruji.com
- Country
- China
- ProdList
- 3224
- Advantage
- 55
- Tel
- 025-84699383-8003 18013301590
- Fax
- 025-84699383-8003
- njduly@126.com
- Country
- China
- ProdList
- 973
- Advantage
- 55
- Tel
- +86-28-85122536 85324413
- Fax
- +86-28-85326443
- market@astatech.cn
- Country
- China
- ProdList
- 8033
- Advantage
- 55
- Tel
- 021-021-33632979 15002134094
- Fax
- 021-33632979
- marketing@targetmol.com
- Country
- China
- ProdList
- 7854
- Advantage
- 58
- Tel
- 021-60136491 15901897992
- Fax
- 021-60136492
- Country
- China
- ProdList
- 455
- Advantage
- 55
- Tel
- 021-61119791,13386096464
- Fax
- 021-50190009
- bzswkf@foxmail.com
- Country
- China
- ProdList
- 3924
- Advantage
- 50
View Lastest Price from Tris(2-carboxyethyl)phosphine hydrochloride manufacturers
- Product
- Tris(2-Carboxyethyl)Phosphine Hydrochloride 51805-45-9
- Price
- US $1.00-0.00/KG
- Min. Order
- 1KG
- Purity
- 99%
- Supply Ability
- 20 tons
- Release date
- 2020-01-15
- Product
- TRIS(2-CARBOXYETHYL)PHOSPHINE HYDROCHLORIDE 51805-45-9
- Price
- US $0.00/kg
- Min. Order
- 1kg
- Purity
- 99%
- Supply Ability
- 2000ton
- Release date
- 2023-08-29
- Product
- Tris(2-carboxyethyl)phosphine hydrochloride 51805-45-9
- Price
- US $8.00-1.00/KG
- Min. Order
- 1KG
- Purity
- 99%
- Supply Ability
- g-kg-tons, free sample is available
- Release date
- 2024-03-29