ChemicalBook > CAS DataBase List > Tris(2-carboxyethyl)phosphine hydrochloride

Tris(2-carboxyethyl)phosphine hydrochloride

Product Name
Tris(2-carboxyethyl)phosphine hydrochloride
CAS No.
51805-45-9
Chemical Name
Tris(2-carboxyethyl)phosphine hydrochloride
Synonyms
TCEP HCL;TCEP Solution;Tris(2-carboxyethyl)phosphine hydrochloride, 98% 1GR;TCEP*HCL;TCEP-HCI;0.5M TCEP;500 MM TCEP;TCEP, HYDROCHLORIDE;)phosphine hydrochL;TCEP-HCl, OMniPur(R)
CBNumber
CB8263816
Molecular Formula
C9H15O6P.ClH
Formula Weight
286.65
MOL File
51805-45-9.mol
More
Less

Tris(2-carboxyethyl)phosphine hydrochloride Property

Melting point:
177 °C
Density 
1.041 g/mL at 25 °C
refractive index 
n20/D 1.367
storage temp. 
2-8°C
solubility 
H2O: 50 mg/mL
form 
powder
pka
7.66(at 25℃)
color 
white
PH
7.0(aqueous solution; pH was adjusted with ammonium hydroxide)
Water Solubility 
Soluble in water and methanol.
Sensitive 
Moisture Sensitive
λmax
λ: 260 nm Amax: 0.05
λ: 280 nm Amax: 0.03
Merck 
14,9085
BRN 
3724376
Stability:
Stable for 1 year from date of purchase as supplied. Solutions in distilled water may be stored at -20°C for up to 1 month.
InChIKey
PBVAJRFEEOIAGW-UHFFFAOYSA-N
CAS DataBase Reference
51805-45-9(CAS DataBase Reference)
More
Less

Safety

Hazard Codes 
C,Xi
Risk Statements 
36/38-36/37/38-34
Safety Statements 
26-37/39-36/37/39-27-45
RIDADR 
UN 3261 8/PG 2
WGK Germany 
2
F 
10
TSCA 
No
HazardClass 
8
PackingGroup 
III
HS Code 
29319090
More
Less

Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Danger
Hazard statements

H314Causes severe skin burns and eye damage

Precautionary statements

P260Do not breathe dust/fume/gas/mist/vapours/spray.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P301+P330+P331IF SWALLOWED: Rinse mouth. Do NOT induce vomiting.

P303+P361+P353IF ON SKIN (or hair): Remove/Take off Immediately all contaminated clothing. Rinse SKIN with water/shower.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

More
Less

N-Bromosuccinimide Price

Sigma-Aldrich
Product number
68957
Product name
Tris(2-carboxyethyl)phosphine hydrochloride
Purity
BioUltra, for electrophoresis, SDS-PAGE tested
Packaging
10g
Price
$1110
Updated
2024/03/01
Sigma-Aldrich
Product number
646547
Product name
Tris(2-carboxyethyl)phosphine hydrochloride solution
Purity
0.5 M, pH 7.0(aqueous solution; pH was adjusted with ammonium hydroxide)
Packaging
10x1ml
Price
$132
Updated
2024/03/01
Sigma-Aldrich
Product number
580567
Product name
TCEP,Hydrochloride,ReagentGrade-CAS51805-45-9-Calbiochem
Packaging
5G
Price
$338
Updated
2024/03/01
Sigma-Aldrich
Product number
580560
Product name
TCEP, Hydrochloride - CAS 51805-45-9 - Calbiochem
Purity
A water-soluble and odorless disulfide-reducing agent that is more stable and effective than DTT.
Packaging
1G
Price
$122
Updated
2022/05/15
TCI Chemical
Product number
T1656
Product name
Tris(2-carboxyethyl)phosphine Hydrochloride
Purity
>98.0%(T)
Packaging
1g
Price
$53
Updated
2024/03/01
More
Less

Tris(2-carboxyethyl)phosphine hydrochloride Chemical Properties,Usage,Production

Description

TCEP HCl (51805-45-9) is a reagent for selective reduction of disulfides in aqueous solutions1-3?with distinct advantages over DTT which are application specific3. For example, spin labels are more stable with TCEP than DTT, Ni2+?(leached from affinity columns) causes rapid oxidation of DTT with no effect on TCEP and for long term storage of proteins TCEP is more stable than DTT with no metal chelator (EGTA) present but DTT is more stable in the presence of metal chelators.3

Chemical Properties

White Crystalline Solid

Uses

TCEP (Tris(2-carboxyethyl)phosphine hydrochloride) is an alternative which is not pungent, more stable and works even at low pH. It is more selective, i.e. do not reduce metals and buried disulfides.

Uses

Reducing agent, specific for disulfidesTris(2-carboxyethyl)phosphine hydrochloride is widely used as a reducing agent to reduce disulfide bonds in proteins and trans-4,5-dihydroxy-1,2-dithiane. It is involved in the reduction of sulfoxides, sulfonyl chlorides, N-oxides, and azides. It plays an important role in the tissue homogenization process for RNA isolation. It acts as a chelating agent and forms complexes with various heavy metal ions such as Zn(II), Cd(II), Pb(II), and Ni(II). Furthermore, it is useful in the labeling of cysteine residues with maleimides.

Uses

Biochemical tool for selective reduction of disulfide bridges at low pH; reductant for redox assays.

Preparation

The preparation of Tris(2-carboxyethyl)phosphine hydrochloride is as follows:Trimethyl 3,3 ', 3 "-phosphine triphenylpropionate (92.5g, 0.28mol) prepared as described above was added to a 250 ml reaction flask under mechanical stirring conditions and a molar ratio of 12mol/(120ml, 1.44mol), the mixture was heated to 90°C, the solid gradually dissolved, continue to heat the reaction 4h, stirring and cooling with ice water, precipitation of white solid, filter, filter cake with distilled water to get white (2-carboxyethyl) phosphine hydrochloride, dried, weighing 61.3g, yield 82.4%

General Description

Tris (2-carboxyethyl) phosphine (TCEP) is very effective in cleaving disulfide bonds in aqueous solution. It dissolves in water and is odorless, unlike other trialkylphosphines (tributylphosphine). It is also less toxic than 2-mercaptoethanol. These advantages make it better than the other reducing agents.

Reactivity Profile

Nucleophilic Substitution by the Phosphorus Atom of TCEP. 1. The phosphorus atom attacks one sulfur atom along the S-S bond. 2. A thioalkoxyphosphonium cation and a sulfhydryl anion are formed. 3. A rapid hydrolysis releases the second sulfhydryl molecule and the phosphine oxide. There are two main steps in the breaking of disulfide bridges and forming a free sulfhydryl using TCEP: cleavage of the S-S bond; oxidation of the phosphine and release of sulfhydryl.

Biochem/physiol Actions

Tris(2-carboxyethyl)phosphine hydrochloride solution reduces the disulfide bonds and leaves other functional groups intact in proteins.

storage

Following reconstitution, aliquot and freeze (-20°C). TCEP retains stability when stored at an acidic or alkaline pH; avoid storage at pH 7.0-8.0. Also avoid exposure to high concentrations of phosphate ions (>150 mM). Stock solutions are stable for up to 3 months at -20°C.

References

1) Fischer?et al.?(1993),?In situ reduction suitable for matrix-assisted laser desorption/ionization and liquid secondary ionization using tris(2-carboxyethyl)phosphine; Rapid Commun. Mass Spectrom.,?7?225 2) Kirley?et al.?(1989),?Reduction and fluorescent labeling of cyst(e)ine-containing proteins for subsequent structural analysis; Anal. Biochem.,?180?231 3) Getz?et al. (1999),?A comparison between the sulfhydryl reductants tris(2-carboxyethyl)phosphine and dithiothreitol for use in protein biochemistry; Anal. Biochem.,?273?73

Tris(2-carboxyethyl)phosphine hydrochloride Preparation Products And Raw materials

Raw materials

Preparation Products

More
Less

Tris(2-carboxyethyl)phosphine hydrochloride Suppliers

Belgium 1 Canada 3 China 395 France 1 Germany 7 India 14 Japan 3 Poland 2 South Korea 1 Switzerland 4 United Kingdom 10 United States 56 Global 497
Suzhou Highfine Biotech Co., Ltd
Tel
0512-68311833 13962195826
Fax
0512-68417696
Email
wuhao@highfine.com
Country
China
ProdList
499
Advantage
75
Suzhou Haiheng Biological Pharmaceutical Co., Ltd.
Tel
0512-65045967 15365387483
Fax
15365387483
Email
sales@haihengpharm.com
Country
China
ProdList
94
Advantage
55
TCI (Shanghai) Development Co., Ltd.
Tel
021-67121386
Fax
021-67121385
Email
Sales-CN@TCIchemicals.com
Country
China
ProdList
24529
Advantage
81
Hubei XinghengKang Chemical Technology Co., Ltd.
Tel
027-88878869 18162384755
Fax
027-88878869
Email
xhkhu2014@163.com
Country
China
ProdList
239
Advantage
58
Hubei xinghengkang Chemical Technology Co., Ltd
Tel
027-88878869 18162384755
Email
969094702@qq.com
Country
China
ProdList
59
Advantage
58
Jiangsu Boquan Biotechnology Co., Ltd.
Tel
15252131412; 15252131412
Email
5954981@qq.com
Country
China
ProdList
2925
Advantage
58
Zhengzhou Runkai Pharmaceutical Technology Co., Ltd
Tel
19137044811; 19137644811
Email
shisx1211@163.com
Country
China
ProdList
503
Advantage
58
Nanjing Pope Bio-tech R&D Co., Ltd
Tel
18951783565 18951783565
Email
sales@popebiotech.com
Country
China
ProdList
1116
Advantage
58
JiangSu Star Science Co.,Ltd
Tel
400-400-605-7200-7200 17312445705
Email
sales022@b2star.com
Country
China
ProdList
3761
Advantage
58
Guangzhou Brand-new Changshi Pharmaceutical Biotechnology Co., Ltd
Tel
19066311943
Email
815989315@qq.com
Country
China
ProdList
648
Advantage
58
J & K SCIENTIFIC LTD.
Tel
010-82848833 400-666-7788
Fax
86-10-82849933
Email
jkinfo@jkchemical.com
Country
China
ProdList
96815
Advantage
76
PharmaBlock Sciences (Nanjing),Inc.
Tel
025-86918202 4000255188
Fax
86-025-86918232
Email
sales@pharmablock.com
Country
China
ProdList
4986
Advantage
55
Meryer (Shanghai) Chemical Technology Co., Ltd.
Tel
021-61259108 18621169109
Fax
86-21-61259102
Email
market03@meryer.com
Country
China
ProdList
40228
Advantage
62
Beijing Donghualituo Techonlogy Development Co.,Ltd.
Tel
010-88425576
Fax
(+86)-10-88425546
Email
sales@dhltchem.com
Country
China
ProdList
793
Advantage
59
Alfa Aesar
Tel
400-6106006
Fax
021-67582001/03/05
Email
saleschina@alfa-asia.com
Country
China
ProdList
30123
Advantage
84
Beijing HwrkChemical Technology Co., Ltd
Tel
010-89508211 18501085097
Fax
010-89508210
Email
sales.bj@hwrkchemical.com
Country
China
ProdList
8418
Advantage
55
Energy Chemical
Tel
021-021-58432009 400-005-6266
Fax
021-58436166
Email
sales8178@energy-chemical.com
Country
China
ProdList
44689
Advantage
61
Wuhan Chemwish Technology Co., Ltd
Tel
86-027-67849912
Fax
86-027-87531808
Email
sales@chemwish.com
Country
China
ProdList
35896
Advantage
56
GL Biochem (Shanghai) Ltd
Tel
21-61263452 13641803416
Fax
86-21-61263399
Email
ymbetter@glbiochem.com
Country
China
ProdList
9981
Advantage
64
Capot Chemical Co., Ltd
Tel
+86 (0) 571 85 58 67 18
Fax
0086-571-85864795
Email
sales@capotchem.com
Country
China
ProdList
18207
Advantage
66
Shanghai Sinch Parmaceuticals Tech. Co. Ltd.
Tel
+86-21-54098501
Fax
+86-21-54096319
Email
sales@sinch.com.cn
Country
China
ProdList
11598
Advantage
64
Jia Xing Isenchem Co.,Ltd
Tel
0573-85285100 18627885956
Fax
0573-85285100
Email
isenchem@163.com
Country
China
ProdList
9413
Advantage
66
Adamas Reagent, Ltd.
Tel
400-6009262 16621234537
Fax
021-64823266
Email
chenyj@titansci.com
Country
China
ProdList
14103
Advantage
59
Accela ChemBio Co.,Ltd.
Tel
021-50795510 4000665055
Fax
021-50795055
Email
sales@accelachem.com
Country
China
ProdList
11655
Advantage
64
Shanghai Hanhong Scientific Co.,Ltd.
Tel
021-54306202 13764082696
Email
info@hanhongsci.com
Country
China
ProdList
42958
Advantage
64
Chemsky（shanghai）International Co.,Ltd.
Tel
021-50135380
Email
shchemsky@sina.com
Country
China
ProdList
32321
Advantage
50
Shandong Xiya Chemical Co., Ltd
Tel
4009903999 13355009207
Fax
0539-6365991
Email
3007715519@qq.com
Country
China
ProdList
18738
Advantage
57
Syntechem Co.,Ltd
Tel
Fax
E-Mail Inquiry
Email
info@syntechem.com
Country
China
ProdList
12984
Advantage
57
Sichuan Kulinan Technology Co., Ltd
Tel
400-1166-196 18981987031
Fax
028-84555506 800101999
Email
cdhxsj@163.com
Country
China
ProdList
11707
Advantage
57
Tianjin heowns Biochemical Technology Co., Ltd.
Tel
400 638 7771
Email
sales@heowns.com
Country
China
ProdList
14435
Advantage
57
Shanghai Sunway Pharmaceutical Technology Co., Ltd
Tel
021-51613915-820 13611835272
Fax
021 51613951
Email
mmwang@sunwaypharm.cn
Country
China
ProdList
9734
Advantage
57
Hunan Hui Bai Shi Biotechnology Co., Ltd.
Tel
0731-85526065 13308475853
Email
ivy@hnhbsj.com
Country
China
ProdList
4552
Advantage
62
Nanjing Vital Chemical Co., Ltd.
Tel
Please Send Email 18652950785
Fax
025-87193546
Email
chemweiao@163.com
Country
China
ProdList
4378
Advantage
65
Spectrum Chemical Manufacturing Corp.
Tel
021-021-021-67601398-809-809-809 15221380277
Fax
021-57711696
Email
marketing_china@spectrumchemical.com
Country
China
ProdList
9658
Advantage
60
Wuhan Fortuna Chemical Co., Ltd
Tel
027-59207852 13308628970
Fax
QQ3130921841
Email
buy@fortunachem.com
Country
China
ProdList
2871
Advantage
58
Dalian Meilun Biotech Co., Ltd.
Tel
0411-62910999 13889544652
Email
meilunui@163.com
Country
China
ProdList
4727
Advantage
58
ShangHai YuanYe Biotechnology Co., Ltd.
Tel
021-61312847 13636370518
Fax
021-55068248
Email
shyysw007@163.com
Country
China
ProdList
4940
Advantage
60
Chengdu Ai Keda Chemical Technology Co., Ltd.
Tel
4008-755-333 18080918076
Fax
028-86757656
Email
800078821@qq.com
Country
China
ProdList
9718
Advantage
55
Shanghai civi chemical technology co.,Ltd
Tel
86-21-34053660
Fax
86-21-34053661
Email
sale@labgogo.com
Country
China
ProdList
9865
Advantage
52
Thermo Fisher Scientific
Tel
800-810-5118
Fax
+86-10-84193589
Email
cnchemical@thermofisher.com
Country
China
ProdList
17770
Advantage
75
Beijing innoChem Science & Technology Co.,Ltd.
Tel
400-810-7969 010-59572699
Fax
010-59572688
Email
ningzi.li@inno-chem.com.cn
Country
China
ProdList
6134
Advantage
55
9ding chemical ( Shanghai) Limited
Tel
4009209199
Fax
86-021-52271987
Email
sales@9dingchem.com
Country
China
ProdList
22514
Advantage
55
Shanghai Aladdin Bio-Chem Technology Co.,LTD
Tel
400-400-6206333 18521732826
Fax
021-50323701
Email
market@aladdin-e.com
Country
China
ProdList
25003
Advantage
65
The future of Shanghai Industrial Co., Ltd.
Tel
021-61552785
Fax
021-55660885
Email
sales@shshiji.com
Country
China
ProdList
9545
Advantage
55
Shanghai Ruji Biology Technology Co., Ltd.
Tel
+86-21-65211385-8001 36031160
Fax
+86-21-65211385-8004
Email
sales@shruji.com
Country
China
ProdList
3224
Advantage
55
Nanjing Dulai Biotechnology Co., Ltd.
Tel
025-84699383-8003 18013301590
Fax
025-84699383-8003
Email
njduly@126.com
Country
China
ProdList
971
Advantage
55
Chengdu AstaTech Trading Co., Ltd./AstaTech (Chengdu) Pharma. Co., Ltd.
Tel
+86-28-85122536 85324413
Fax
+86-28-85326443
Email
market@astatech.cn
Country
China
ProdList
8026
Advantage
55
TargetMol Chemicals Inc.
Tel
021-021-33632979 15002134094
Fax
021-33632979
Email
marketing@targetmol.com
Country
China
ProdList
7783
Advantage
58
ShangHai Zexin Chemical Technology Co., Ltd.
Tel
021-60136491 15901897992
Fax
021-60136492
Country
China
ProdList
455
Advantage
55
Shanghai BeiZhuo Biotech Co., Ltd.
Tel
021-61119791,13386096464
Fax
021-50190009
Email
bzswkf@foxmail.com
Country
China
ProdList
3921
Advantage
50
More
Less

View Lastest Price from Tris(2-carboxyethyl)phosphine hydrochloride manufacturers

Hebei Zhuanglai Chemical Trading Co.,Ltd
Product
Tris(2-carboxyethyl)phosphine hydrochloride 51805-45-9
Price
US $10.00/kg
Min. Order
1kg
Purity
99%
Supply Ability
20ton
Release date
2024-05-21
WUHAN FORTUNA CHEMICAL CO., LTD
Product
Tris(2-carboxyethyl)phosphine Hydrochloride 51805-45-9
Price
US $0.00-0.00/kg
Min. Order
100kg
Purity
98%min
Supply Ability
50kg
Release date
2024-06-03
Apeloa production Co.,Limited
Product
Tris(2-carboxyethyl)phosphine Hydrochloride 51805-45-9
Price
US $1.00/g
Min. Order
1g
Purity
99%
Supply Ability
3000
Release date
2024-06-05

51805-45-9, Tris(2-carboxyethyl)phosphine hydrochlorideRelated Search:

Tris(2-butoxyethyl) phosphate Tris(4-methoxyphenyl)phosphine TRIS(2-METHOXYPHENYL)PHOSPHINE Tris(3-methoxyphenyl)phosphine Tris(4-fluorophenyl)phosphine SPINETORAM 4'-Hydroxyacetophenone Decamethylcyclopentasiloxane Hydroxyethyl Cellulose Tris(2-chloroethyl) phosphate 2-Bromoethanol 4-Ethylaniline Phosphoric acid Maleimide Thiourea Fudosteine TRIISOBUTYLSILANE TRIS(2-HYDROXYETHYL)METHYLAMMONIUM HYDROXIDE

  • Tris(2-carboxyethyl)phosphine hydrochloride solution,TCEP
  • TCEP-HCl, OMniPur(R)
  • TRIS(2-CARBOXYETHYL)PHOSPHINE:DCL (TCEP)
  • Tris(carboxyethyl)phosphine.HC
  • Tris(2-carboxyethyl)phosphine hydrochloride, 0.5M soln in water
  • Tris(2-carboxyethyl)phosphine hydrochloride, 98% 1GR
  • Tris(2-carboxyethyl)phosphine hydrochloride, 95%, 0.5M soln. in water
  • 500 MM TCEP
  • TCEP Solution
  • 3,3',3''-phosphinetriyltripropanoic acid hydrochloride
  • TRIS(2-CYANOETHYL)PHOSPHINE,TCEP.HCL)
  • Three(2- Suo Yiji)phosphine hydrochloride
  • TCEP.HCLTris(2-Carboxyethyl)Phosphine Hydrochloride
  • Propanoic acid,3,3',3''-phosphinylidynetris-, hydrochloride (1:1)
  • Tris(2-carboxyethyl)phosphine hydrochloride powder, >=98%
  • Tris(2-carboxyethyl)phosphine hydrochloride solution
  • 3,3',3''-PHOSPHINETRIYL-TRIPROPIONIC ACID HYDROCHLORIDE
  • 3,3',3''-PHOSPHINIDYNE-TRIPROPIONIC ACID HYDROCHLORIDE
  • TCEP HCL
  • TCEP*HCL
  • TCEP, HYDROCHLORIDE
  • TRI(2-CARBOXYETHYL)PHOSPHINE HYDROCHLORIDE
  • TRIS(2-CARBOXYETHYL)PHOSPHINE, HCL
  • TRIS(2-CARBOXYETHYL)PHOSPHINE HYDROCHLORIDE
  • TRIS(CARBOXYETHYL)PHOSPHINE HYDROCHLORIDE
  • TRIS(CARBOXYETHYL)PHOSPHINE &
  • 0.5M TCEP
  • TRIS(2-CARBOXYETHYL)PHOSPHINE HYDROCHLORIDE 99%
  • TRIS-(2-CARBOXYETHYL)PHOSPHINE HYDROCHLORIDE (TCEP HCL)
  • Tris(2-carboxyethyl)phosphine-D16DCl
  • Tris-(2-carboxyethy)phosphine hydrochloride
  • Tris(2-carboxyethyl)phosphine,hydrochloride,99%
  • Tris(2-carboxyethyl)phosphonium Hydrochloride
  • TRIS-(2-CARBOXYETHYL)PHOSPHINE HYDROCHLORIDE (TCEP)
  • 3,3',3''-Phosphinidynetrispropanoic Acid Hydrochloride
  • Tris(2-carboxyethyl)phosphine solution hydrochloride
  • Tris(2-carboxyethyl)phosphine hydrochloride,98%
  • Ris(2-Carboxyethyl)Phosphinehydrochloride
  • TRIS(2-CARBOXYETHYL)PHOSPHINE HYDROC
  • TCEP, Hydrochloride, Reagent Grade
  • Tris(2-carboxyethyl)phosphine, hydrochloride, 99% TCEP
  • Protein And Peptide Recombination
  • Tris(carboxyethyl)phosphine HCl
  • Protein And Peptide Recombination (Tcep·Hcl)
  • Tris(2-carboxyethyl)phosphine hydrochloride (TCEP HCl salt)
  • TCEP.HCL Tris(2-Carboxyethyl)Phosphine Hydrochloride,98%
  • )phosphine hydrochL
  • 3-bis(2-Carboxyethyl)phosphanylpropanoic acid hydrochloride (0.5M solution in water)
  • TCEP Hydrochloride (0.5M in water)
  • TRIS(2-CARBOXYETHYL)PHOSPHINE HYDROCHLORIDE CAS 51805-45-9
  • Tris 2-carboxyethyl phospene DCL (TCEP) D16
  • Tris(2-carboxyethyl) Phosphine Hydrochloride (TCEP) extrapure AR, 98%
  • TCEP, Hydrochloride salt
  • Tris(2-carboxyethyl)phosphine hydrochlo
  • Tris (2-carbonylethyl) phosphate hydrochloride
  • TCEP-HCI
  • Tri (2-carbonylethyl) phosphate hydrochloride
  • Tri (2-carbonylethyl) phosphine hydrochloride