Hoechst 33258
- Product Name
- Hoechst 33258
- CAS No.
- 23491-45-4
- Chemical Name
- Hoechst 33258
- Synonyms
- BBIH;BXI-72;H33258;abs9129;Ht-33258;hoe33058;NSC334072;HOE 33258;nsc-322921;33258hoechst
- CBNumber
- CB8329486
- Molecular Formula
- C25H25ClN6O
- Formula Weight
- 460.97
- MOL File
- 23491-45-4.mol
Hoechst 33258 Property
- Melting point:
- 314 °C
- Density
- 1.5099 (rough estimate)
- refractive index
- 1.6300 (estimate)
- storage temp.
- -20°C
- solubility
- H2O: 10 mg/mL
- form
- powder
- color
- Yellow to Very Dark Yellow
- Water Solubility
- SOLUBLE
- BRN
- 4088183
- Stability:
- Hygroscopic
- CAS DataBase Reference
- 23491-45-4(CAS DataBase Reference)
- EPA Substance Registry System
- Phenol, 4-[5-(4-methyl-1-piperazinyl)[2,5'-bi-1H-benzimidazol]-2'-yl]-, trihydrochloride (23491-45-4)
Safety
- Hazard Codes
- Xn
- Risk Statements
- 22-36/38-36/37/38-20/21/22
- Safety Statements
- 26-36-24/25-23
- WGK Germany
- 3
- RTECS
- SM1140500
- F
- 3-8-10
- HS Code
- 32129000
- Toxicity
- dog,LD,intravenous,> 22400ug/kg (22.4mg/kg),LUNGS, THORAX, OR RESPIRATION: DYSPNEASENSE ORGANS AND SPECIAL SENSES: LACRIMATION: EYEGASTROINTESTINAL: CHANGES IN STRUCTURE OR FUNCTION OF SALIVARY GLANDS,National Technical Information Service. Vol. PB84-171206,
Hazard and Precautionary Statements (GHS)
- Symbol(GHS)
-
- Signal word
- Warning
- Hazard statements
-
H302Harmful if swallowed
H315Causes skin irritation
H319Causes serious eye irritation
- Precautionary statements
-
P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.
N-Bromosuccinimide Price
- Product number
- 382061
- Product name
- Bisbenzimide H 33258 Fluorochrome, Trihydrochloride - CAS 23491-45-4 - Calbiochem
- Purity
- Membrane-permeable, adenine-thymine-specific fluorescent stain.
- Packaging
- 1g
- Price
- $962
- Updated
- 2024/03/01
- Product number
- 14530
- Product name
- bisBenzimide H 33258
- Purity
- for fluorescence, ≥98.0% (HPLC)
- Packaging
- 100mg
- Price
- $105
- Updated
- 2024/03/01
- Product number
- 14530
- Product name
- bisBenzimide H 33258
- Purity
- for fluorescence, ≥98.0% (HPLC)
- Packaging
- 500mg
- Price
- $372
- Updated
- 2024/03/01
- Product number
- H1343
- Product name
- 2'-(4-Hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bibenzimidazole Trihydrochloride Hydrate[for Biochemical Research]
- Purity
- >98.0%(HPLC)
- Packaging
- 25mg
- Price
- $65
- Updated
- 2024/03/01
- Product number
- 16756
- Product name
- Hoechst 33258
- Purity
- ≥95%
- Packaging
- 50mg
- Price
- $47
- Updated
- 2024/03/01
Hoechst 33258 Chemical Properties,Usage,Production
Chemical Properties
yellow to yellow-green powder
Uses
Hoechst 33258 is a Hoechst stain used to stain DNA. It is part of a family of fluorescent dyes. Dyes and metabolites.
Uses
bisBenzimide H 33258 is useful for staining DNA, chromosomes and nuclei. bisBenzimide H 33258 may be used for fluorescence microscopy or flow cytometry.
Excitation max. =?346 nm
Emission max. =?460 nm
Uses
Hoechst 33258 is useful for staining DNA, chromosomes and nuclei. The nucleic acid binds to the minor groove of DNA at AT-rich sequences. This dye is commonly used for determining the DNA content of viable cells without detergent treatment or fixation and for fluorescence
General Description
Membrane-permeable, adenine-thymine-specific fluorescent stain. Useful for staining DNA, chromosomes, and nuclei. Increases the rate of channel-mediated Ca2+ efflux from junctional cytoplasmic reticulum vesicles. Can also be used to detect mycoplasma contamination in tissue culture. H 33258 and the related compound H 33342 (Cat. No. 382065) have been used in conjunction with RNA aptamers in the 5′ untranslated region (UTR) to control expression of transfected gene constructs.
Biochem/physiol Actions
bisBenzimide H 33258 is a potent and selective Bcl-XL inhibitor. bisBenzimide H 33258 is a membrane-permeable, fluorescent DNA stain with low cytotoxicity that intercalate in A-T regions of DNA.
storage
+4°C (desiccate)
Hoechst 33258 Preparation Products And Raw materials
Raw materials
Preparation Products
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View Lastest Price from Hoechst 33258 manufacturers
- Product
- Hoechst 33258 23491-45-4
- Price
- US $0.00/KG
- Min. Order
- 1KG
- Purity
- 99%
- Supply Ability
- 50000KG/month
- Release date
- 2023-09-28
- Product
- Hoechst 33258 23491-45-4
- Price
- US $1.00/KG
- Min. Order
- 1KG
- Purity
- 99%
- Supply Ability
- 25kg
- Release date
- 2019-07-12