Fluorescamine Property

Melting point:

153-157 °C (lit.)

Boiling point:

381.08°C (rough estimate)

Density 

1.2661 (rough estimate)

refractive index 

1.5447 (estimate)

Flash point:

-17 °C

storage temp. 

room temp

solubility 

acetonitrile: soluble

form 

powder

color 

off-white to yellow

Water Solubility 

Soluble in acetone, ethanol, chloroform, dimethylsulfoxide and acetonitrile. Slightly soluble in water.

Merck 

14,4158

BRN 

921143

Stability:

Stable. Incompatible with strong oxidizing agents.

CAS DataBase Reference

38183-12-9(CAS DataBase Reference)

NIST Chemistry Reference

Spiro[furan-2(3h),1'(3'h)-isobenzofuran]-3,3'-dione, 4-phenyl-(38183-12-9)

Safety

Hazard Codes 

F,Xi

Risk Statements 

11-36/38-66-67-36-36/37/38

Safety Statements 

22-24/25-36/37-33-29-26-16-9-37/39

RIDADR 

UN 1090 3/PG 2

WGK Germany 

3

F 

10-21

HS Code 

29322090

Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Warning

Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

Precautionary statements

P280Wear protective gloves/protective clothing/eye protection/face protection.

P302+P352IF ON SKIN: wash with plenty of soap and water.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

Fluorescamine Chemical Properties,Usage,Production

Chemical Properties

white powder

Uses

For fluorescent detection of amino acids, peptides and proteins.Fluorescamine plays an important role as a reagent for the detection of amines, peptides and proteins. It is also used as a substrate for measuring protease activity. It is utilized in the detection and quantitation of residual aminopenicillins and sulfonamides in honey by HPLC. It finds application in the determination of lisinopril in human plasma and urine.

Uses

Fluorescamine is a non-fluorescent reagent that reacts readily under mild conditions with primary amines in amino acids and peptides to form stable, highly fluorescent compounds. Reaction takes place under mild conditions. Low background due to hydrolysis.

Uses

Analytical reagent.

Synthesis Reference(s)

Journal of the American Chemical Society, 94, p. 5927, 1972 DOI: 10.1021/ja00771a084

Purification Methods

Fluram is a non-fluorescent reagent that reacts with primary amines to form highly fluorescent compounds. Purify it by dissolving (~1g) in Et2O/*C6H6 (1:1, 180 mL), washing with 1% aqueous NaHCO3 (50mL), drying (Na2SO4), and evaporating in a vacuum. Dissolve the residue in warm CH2Cl2 (5mL), dilute with Et2O (12mL) and refrigerate. Collect the solid and dry it in a vacuum. IR (CHCl3): 1810, 1745, 1722, 1625 and 1600 cm-1, and 1HNMR (CDCl3): 8.71 (s, -OHC=). [Weigele et max al. J Am Chem Soc 94 5927 1972, Weigele et al. J Org Chem 41 388 1976, Lai Methods Enzymol 47 236 1977.] Forskolin (Colforsin, Coleonol, 5-[acetyloxy]-3-ethenyldodecahydro-6,10,10b-trihydroxy-3,4a,7,7, 10a-penta-methyl-[3R -{3