N-(5-CHLORO-2,4-DIMETHOXYPHENYL)-N'-(5-METHYL-3-ISOXAZOLYL)-UREA Property

Boiling point:

385.0±42.0 °C(Predicted)

Density 

1.403

storage temp. 

room temp

solubility 

DMSO: >10mg/mL

form 

White solid

pka

11.72±0.70(Predicted)

color 

white to off-white

Safety

WGK Germany 

3

Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Warning

Hazard statements

H302Harmful if swallowed

H315Causes skin irritation

H320Causes eye irritation

H335May cause respiratory irritation

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P301+P312IF SWALLOWED: call a POISON CENTER or doctor/physician IF you feel unwell.

P302+P352IF ON SKIN: wash with plenty of soap and water.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

N-(5-CHLORO-2,4-DIMETHOXYPHENYL)-N'-(5-METHYL-3-ISOXAZOLYL)-UREA Chemical Properties,Usage,Production

Uses

PNU 120596 is a type II positive allosteric modulator.

Definition

ChEBI: 1-(5-chloro-2,4-dimethoxyphenyl)-3-(5-methyl-3-isoxazolyl)urea is a member of ureas.

Biological Activity

Positive allosteric modulator of α 7 neuronal nicotinic acetylcholine receptors (EC 50 = 216 nM), with no detectable effect on α 4 β 2, α 3 β 4 and α 9 α 10 receptors. Active in vivo following systemic administration.

Biochem/physiol Actions

An allosteric modulator of α7 nicotinic receptors, N-(5-chloro-2,4-dimethoxyphenyl)-N′-(5-methyl-3-isoxazolyl)-urea (PNU-120596), causes conformational changes in the extracellular ligand binding domain similar to those caused by acetylcholinePNU-120596 is a positive allosteric modulator selective for the α7 nicotinic acetylcholine receptor. PNU-120596 produces no detectable change in currents mediated by α4β2, α3β4, α9α10 nAChRs. It increases channel mean open time, but does not affect ion selectivity. It does not bind at the agonist binding site, but induces conformational changes similar to the natural effector.

storage

Desiccate at +4°C

References

[1] hurst r s, hajós m, raggenbass m, et al. a novel positive allosteric modulator of the α7 neuronal nicotinic acetylcholine receptor: in vitro and in vivo characterization. the journal of neuroscience, 2005, 25(17): 4396-4405.
[2] bertrand d, gopalakrishnan m. allosteric modulation of nicotinic acetylcholine receptors. biochemical pharmacology, 2007, 74(8): 1155-1163.